Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10375 and BWI76_RS10380 are separated by 13 nucleotides BWI76_RS10380 and BWI76_RS10385 are separated by 142 nucleotides BWI76_RS10385 and BWI76_RS10390 are separated by 4 nucleotides
BWI76_RS10375: BWI76_RS10375 - polar amino acid ABC transporter inner membrane subunit, at 2,196,857 to 2,197,522
_RS10375
BWI76_RS10380: BWI76_RS10380 - polar amino acid ABC transporter inner membrane subunit, at 2,197,536 to 2,198,207
_RS10380
BWI76_RS10385: BWI76_RS10385 - MmgE/PrpD family protein, at 2,198,350 to 2,199,759
_RS10385
BWI76_RS10390: BWI76_RS10390 - amino acid ABC transporter, at 2,199,764 to 2,200,588
_RS10390
Position (kb)
2198
2199
2200 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2197.551 kb on - strand at 2197.617 kb on - strand, within BWI76_RS10380 at 2197.683 kb on - strand, within BWI76_RS10380 at 2198.137 kb on + strand, within BWI76_RS10380 at 2198.140 kb on + strand at 2198.209 kb on + strand at 2198.262 kb on + strand at 2198.433 kb on + strand at 2198.606 kb on - strand, within BWI76_RS10385 at 2198.832 kb on - strand, within BWI76_RS10385 at 2199.436 kb on - strand, within BWI76_RS10385 at 2199.577 kb on + strand, within BWI76_RS10385 at 2199.805 kb on + strand at 2199.813 kb on - strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.820 kb on + strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2199.828 kb on - strand at 2200.149 kb on - strand, within BWI76_RS10390 at 2200.233 kb on + strand, within BWI76_RS10390 at 2200.235 kb on - strand, within BWI76_RS10390 at 2200.299 kb on + strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.307 kb on - strand, within BWI76_RS10390 at 2200.337 kb on + strand, within BWI76_RS10390 at 2200.341 kb on - strand, within BWI76_RS10390 at 2200.371 kb on - strand, within BWI76_RS10390 at 2200.387 kb on + strand, within BWI76_RS10390 at 2200.398 kb on + strand, within BWI76_RS10390 at 2200.399 kb on + strand at 2200.407 kb on - strand, within BWI76_RS10390 at 2200.408 kb on - strand, within BWI76_RS10390 at 2200.408 kb on - strand, within BWI76_RS10390 at 2200.413 kb on - strand, within BWI76_RS10390 at 2200.413 kb on - strand, within BWI76_RS10390 at 2200.438 kb on - strand, within BWI76_RS10390 at 2200.452 kb on + strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.460 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand, within BWI76_RS10390 at 2200.465 kb on - strand at 2200.467 kb on + strand, within BWI76_RS10390 at 2200.470 kb on - strand, within BWI76_RS10390 at 2200.470 kb on - strand, within BWI76_RS10390 at 2200.475 kb on - strand, within BWI76_RS10390 at 2200.475 kb on - strand, within BWI76_RS10390 at 2200.514 kb on + strand at 2200.522 kb on - strand at 2200.548 kb on + strand at 2200.598 kb on - strand at 2200.650 kb on - strand at 2200.650 kb on - strand at 2200.694 kb on - strand at 2200.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 2,197,551 - -1.0 2,197,617 - BWI76_RS10380 0.12 +0.5 2,197,683 - BWI76_RS10380 0.22 +0.1 2,198,137 + BWI76_RS10380 0.89 +0.3 2,198,140 + -0.3 2,198,209 + -0.3 2,198,262 + -0.0 2,198,433 + -0.5 2,198,606 - BWI76_RS10385 0.18 -0.9 2,198,832 - BWI76_RS10385 0.34 -1.4 2,199,436 - BWI76_RS10385 0.77 -1.3 2,199,577 + BWI76_RS10385 0.87 -1.2 2,199,805 + +0.1 2,199,813 - -0.3 2,199,820 + +1.1 2,199,820 + +0.4 2,199,820 + -0.3 2,199,820 + -0.1 2,199,820 + +0.4 2,199,820 + -0.4 2,199,828 - -1.2 2,199,828 - +0.4 2,199,828 - -0.5 2,199,828 - +1.2 2,200,149 - BWI76_RS10390 0.47 -0.1 2,200,233 + BWI76_RS10390 0.57 -0.2 2,200,235 - BWI76_RS10390 0.57 +1.1 2,200,299 + BWI76_RS10390 0.65 -1.1 2,200,307 - BWI76_RS10390 0.66 -2.1 2,200,307 - BWI76_RS10390 0.66 -1.8 2,200,307 - BWI76_RS10390 0.66 -0.3 2,200,337 + BWI76_RS10390 0.69 -0.4 2,200,341 - BWI76_RS10390 0.70 +0.1 2,200,371 - BWI76_RS10390 0.74 -0.5 2,200,387 + BWI76_RS10390 0.76 -2.5 2,200,398 + BWI76_RS10390 0.77 -1.8 2,200,399 + +2.1 2,200,407 - BWI76_RS10390 0.78 +0.7 2,200,408 - BWI76_RS10390 0.78 +0.3 2,200,408 - BWI76_RS10390 0.78 -0.1 2,200,413 - BWI76_RS10390 0.79 -1.3 2,200,413 - BWI76_RS10390 0.79 -0.6 2,200,438 - BWI76_RS10390 0.82 -0.1 2,200,452 + BWI76_RS10390 0.83 +0.1 2,200,460 - BWI76_RS10390 0.84 -1.3 2,200,460 - BWI76_RS10390 0.84 +0.0 2,200,460 - BWI76_RS10390 0.84 -0.1 2,200,460 - BWI76_RS10390 0.84 -1.0 2,200,460 - BWI76_RS10390 0.84 -1.2 2,200,460 - BWI76_RS10390 0.84 -0.0 2,200,460 - BWI76_RS10390 0.84 +0.3 2,200,465 - BWI76_RS10390 0.85 -0.2 2,200,465 - BWI76_RS10390 0.85 -0.7 2,200,465 - +2.1 2,200,467 + BWI76_RS10390 0.85 -0.6 2,200,470 - BWI76_RS10390 0.86 -0.2 2,200,470 - BWI76_RS10390 0.86 +0.1 2,200,475 - BWI76_RS10390 0.86 +0.3 2,200,475 - BWI76_RS10390 0.86 +1.2 2,200,514 + +0.1 2,200,522 - +1.1 2,200,548 + +0.5 2,200,598 - -0.1 2,200,650 - -0.3 2,200,650 - +0.3 2,200,694 - -0.5 2,200,694 - -1.6
Or see this region's nucleotide sequence