Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09565 and BWI76_RS09570 are separated by 11 nucleotides BWI76_RS09570 and BWI76_RS09575 are separated by 34 nucleotides BWI76_RS09575 and BWI76_RS09580 overlap by 93 nucleotides
BWI76_RS09565: BWI76_RS09565 - hybrid-cluster NAD(P)-dependent oxidoreductase, at 2,000,768 to 2,001,736
_RS09565
BWI76_RS09570: BWI76_RS09570 - hydroxylamine reductase, at 2,001,748 to 2,003,400
_RS09570
BWI76_RS09575: BWI76_RS09575 - hypothetical protein, at 2,003,435 to 2,003,638
_RS09575
BWI76_RS09580: BWI76_RS09580 - surface protein, at 2,003,546 to 2,004,445
_RS09580
Position (kb)
2001
2002
2003
2004 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2000.916 kb on - strand, within BWI76_RS09565 at 2000.965 kb on + strand, within BWI76_RS09565 at 2001.280 kb on + strand, within BWI76_RS09565 at 2001.288 kb on - strand, within BWI76_RS09565 at 2001.446 kb on - strand, within BWI76_RS09565 at 2001.562 kb on + strand, within BWI76_RS09565 at 2001.966 kb on + strand, within BWI76_RS09570 at 2002.022 kb on + strand, within BWI76_RS09570 at 2002.096 kb on - strand, within BWI76_RS09570 at 2002.134 kb on - strand, within BWI76_RS09570 at 2002.249 kb on - strand, within BWI76_RS09570 at 2002.592 kb on + strand, within BWI76_RS09570 at 2002.643 kb on + strand, within BWI76_RS09570 at 2002.668 kb on + strand, within BWI76_RS09570 at 2002.859 kb on + strand, within BWI76_RS09570 at 2003.335 kb on - strand at 2003.350 kb on + strand at 2003.373 kb on + strand at 2003.799 kb on - strand, within BWI76_RS09580 at 2003.889 kb on - strand, within BWI76_RS09580 at 2003.908 kb on + strand, within BWI76_RS09580 at 2003.908 kb on + strand, within BWI76_RS09580 at 2003.916 kb on - strand, within BWI76_RS09580 at 2003.916 kb on - strand, within BWI76_RS09580 at 2003.926 kb on - strand, within BWI76_RS09580 at 2003.974 kb on + strand, within BWI76_RS09580 at 2003.995 kb on + strand, within BWI76_RS09580 at 2003.995 kb on + strand, within BWI76_RS09580 at 2004.003 kb on - strand, within BWI76_RS09580 at 2004.003 kb on - strand, within BWI76_RS09580 at 2004.108 kb on + strand, within BWI76_RS09580 at 2004.116 kb on - strand, within BWI76_RS09580 at 2004.116 kb on - strand, within BWI76_RS09580 at 2004.123 kb on - strand, within BWI76_RS09580 at 2004.198 kb on + strand, within BWI76_RS09580 at 2004.198 kb on + strand, within BWI76_RS09580 at 2004.203 kb on + strand, within BWI76_RS09580 at 2004.206 kb on - strand, within BWI76_RS09580 at 2004.206 kb on - strand, within BWI76_RS09580 at 2004.226 kb on + strand, within BWI76_RS09580 at 2004.231 kb on + strand, within BWI76_RS09580 at 2004.307 kb on - strand, within BWI76_RS09580 at 2004.372 kb on + strand at 2004.391 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 2,000,916 - BWI76_RS09565 0.15 -0.9 2,000,965 + BWI76_RS09565 0.20 -0.4 2,001,280 + BWI76_RS09565 0.53 -0.1 2,001,288 - BWI76_RS09565 0.54 +0.2 2,001,446 - BWI76_RS09565 0.70 -0.9 2,001,562 + BWI76_RS09565 0.82 +0.2 2,001,966 + BWI76_RS09570 0.13 -0.2 2,002,022 + BWI76_RS09570 0.17 -0.1 2,002,096 - BWI76_RS09570 0.21 -0.7 2,002,134 - BWI76_RS09570 0.23 +0.8 2,002,249 - BWI76_RS09570 0.30 -0.1 2,002,592 + BWI76_RS09570 0.51 +1.1 2,002,643 + BWI76_RS09570 0.54 +2.1 2,002,668 + BWI76_RS09570 0.56 -1.7 2,002,859 + BWI76_RS09570 0.67 +1.7 2,003,335 - -1.9 2,003,350 + -0.2 2,003,373 + +1.2 2,003,799 - BWI76_RS09580 0.28 +0.5 2,003,889 - BWI76_RS09580 0.38 -0.2 2,003,908 + BWI76_RS09580 0.40 +1.0 2,003,908 + BWI76_RS09580 0.40 -1.2 2,003,916 - BWI76_RS09580 0.41 +0.5 2,003,916 - BWI76_RS09580 0.41 -0.3 2,003,926 - BWI76_RS09580 0.42 +0.4 2,003,974 + BWI76_RS09580 0.48 -0.0 2,003,995 + BWI76_RS09580 0.50 -0.1 2,003,995 + BWI76_RS09580 0.50 -0.3 2,004,003 - BWI76_RS09580 0.51 -0.6 2,004,003 - BWI76_RS09580 0.51 -0.9 2,004,108 + BWI76_RS09580 0.62 +0.3 2,004,116 - BWI76_RS09580 0.63 -0.6 2,004,116 - BWI76_RS09580 0.63 +0.3 2,004,123 - BWI76_RS09580 0.64 +1.8 2,004,198 + BWI76_RS09580 0.72 -0.6 2,004,198 + BWI76_RS09580 0.72 -0.5 2,004,203 + BWI76_RS09580 0.73 +0.2 2,004,206 - BWI76_RS09580 0.73 +1.4 2,004,206 - BWI76_RS09580 0.73 -0.6 2,004,226 + BWI76_RS09580 0.76 +0.0 2,004,231 + BWI76_RS09580 0.76 +0.0 2,004,307 - BWI76_RS09580 0.85 -3.8 2,004,372 + +1.1 2,004,391 + -1.1
Or see this region's nucleotide sequence