Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09280

Experiment: Paraquat dichloride 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09275 and BWI76_RS09280 are separated by 3 nucleotidesBWI76_RS09280 and BWI76_RS09285 are separated by 95 nucleotidesBWI76_RS09285 and BWI76_RS09290 overlap by 1 nucleotidesBWI76_RS09290 and BWI76_RS09295 are separated by 3 nucleotidesBWI76_RS09295 and BWI76_RS09300 overlap by 20 nucleotides BWI76_RS09275: BWI76_RS09275 - major capsid protein, at 1,953,906 to 1,954,958 _RS09275 BWI76_RS09280: BWI76_RS09280 - terminase endonuclease subunit, at 1,954,962 to 1,955,615 _RS09280 BWI76_RS09285: BWI76_RS09285 - hypothetical protein, at 1,955,711 to 1,956,175 _RS09285 BWI76_RS09290: BWI76_RS09290 - phage Tail Protein X, at 1,956,175 to 1,956,378 _RS09290 BWI76_RS09295: BWI76_RS09295 - hypothetical protein, at 1,956,382 to 1,956,597 _RS09295 BWI76_RS09300: BWI76_RS09300 - phage lysozyme, at 1,956,578 to 1,957,087 _RS09300 Position (kb) 1954 1955 1956Strain fitness (log2 ratio) -3 -2 -1 0 1at 1954.046 kb on - strand, within BWI76_RS09275at 1954.208 kb on - strand, within BWI76_RS09275at 1954.310 kb on + strand, within BWI76_RS09275at 1954.325 kb on - strand, within BWI76_RS09275at 1954.356 kb on - strand, within BWI76_RS09275at 1954.620 kb on - strand, within BWI76_RS09275at 1954.908 kb on - strandat 1955.050 kb on + strand, within BWI76_RS09280at 1955.129 kb on + strand, within BWI76_RS09280at 1955.441 kb on + strand, within BWI76_RS09280at 1955.517 kb on + strand, within BWI76_RS09280at 1955.517 kb on + strand, within BWI76_RS09280at 1955.985 kb on - strand, within BWI76_RS09285at 1955.989 kb on + strand, within BWI76_RS09285at 1956.256 kb on - strand, within BWI76_RS09290at 1956.335 kb on - strand, within BWI76_RS09290at 1956.443 kb on - strand, within BWI76_RS09295at 1956.467 kb on + strand, within BWI76_RS09295at 1956.467 kb on + strand, within BWI76_RS09295at 1956.486 kb on + strand, within BWI76_RS09295

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Per-strain Table

Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml
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1,954,046 - BWI76_RS09275 0.13 +0.3
1,954,208 - BWI76_RS09275 0.29 -1.1
1,954,310 + BWI76_RS09275 0.38 +1.1
1,954,325 - BWI76_RS09275 0.40 +0.5
1,954,356 - BWI76_RS09275 0.43 -0.3
1,954,620 - BWI76_RS09275 0.68 +0.0
1,954,908 - +0.5
1,955,050 + BWI76_RS09280 0.13 -1.4
1,955,129 + BWI76_RS09280 0.26 -0.9
1,955,441 + BWI76_RS09280 0.73 +0.1
1,955,517 + BWI76_RS09280 0.85 -3.4
1,955,517 + BWI76_RS09280 0.85 -1.4
1,955,985 - BWI76_RS09285 0.59 -0.8
1,955,989 + BWI76_RS09285 0.60 -1.6
1,956,256 - BWI76_RS09290 0.40 +0.1
1,956,335 - BWI76_RS09290 0.78 -0.3
1,956,443 - BWI76_RS09295 0.28 +0.0
1,956,467 + BWI76_RS09295 0.39 -1.5
1,956,467 + BWI76_RS09295 0.39 +0.4
1,956,486 + BWI76_RS09295 0.48 -0.0

Or see this region's nucleotide sequence