Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05455 and BWI76_RS05460 are separated by 132 nucleotides BWI76_RS05460 and BWI76_RS05465 are separated by 245 nucleotides BWI76_RS05465 and BWI76_RS05470 are separated by 182 nucleotides
BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226
_RS05455
BWI76_RS05460: BWI76_RS05460 - tRNA-Asp, at 1,145,359 to 1,145,435
_RS05460
BWI76_RS05465: BWI76_RS05465 - amidohydrolase, at 1,145,681 to 1,146,451
_RS05465
BWI76_RS05470: BWI76_RS05470 - acyl-CoA dehydrogenase, at 1,146,634 to 1,149,078
_RS05470
Position (kb)
1145
1146
1147 Strain fitness (log2 ratio)
-1
0
1 at 1145.308 kb on + strand at 1145.520 kb on + strand at 1145.628 kb on - strand at 1145.997 kb on - strand, within BWI76_RS05465 at 1146.396 kb on - strand at 1146.490 kb on + strand at 1146.982 kb on - strand, within BWI76_RS05470 at 1147.030 kb on + strand, within BWI76_RS05470 at 1147.132 kb on - strand, within BWI76_RS05470 at 1147.231 kb on + strand, within BWI76_RS05470 at 1147.317 kb on + strand, within BWI76_RS05470 at 1147.347 kb on - strand, within BWI76_RS05470 at 1147.395 kb on - strand, within BWI76_RS05470 at 1147.421 kb on - strand, within BWI76_RS05470 at 1147.428 kb on + strand, within BWI76_RS05470
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 1,145,308 + -0.5 1,145,520 + -1.2 1,145,628 - +1.0 1,145,997 - BWI76_RS05465 0.41 +1.0 1,146,396 - +1.1 1,146,490 + -0.1 1,146,982 - BWI76_RS05470 0.14 -0.8 1,147,030 + BWI76_RS05470 0.16 -0.7 1,147,132 - BWI76_RS05470 0.20 -0.7 1,147,231 + BWI76_RS05470 0.24 +0.2 1,147,317 + BWI76_RS05470 0.28 -0.1 1,147,347 - BWI76_RS05470 0.29 +0.3 1,147,395 - BWI76_RS05470 0.31 -0.5 1,147,421 - BWI76_RS05470 0.32 -0.6 1,147,428 + BWI76_RS05470 0.32 -0.3
Or see this region's nucleotide sequence