Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05435 and BWI76_RS05440 are separated by 71 nucleotides BWI76_RS05440 and BWI76_RS05445 are separated by 32 nucleotides BWI76_RS05445 and BWI76_RS05450 overlap by 4 nucleotides BWI76_RS05450 and BWI76_RS05455 are separated by 65 nucleotides
BWI76_RS05435: BWI76_RS05435 - murein transglycosylase D, at 1,141,016 to 1,142,383
_RS05435
BWI76_RS05440: BWI76_RS05440 - hydroxyacylglutathione hydrolase, at 1,142,455 to 1,143,210
_RS05440
BWI76_RS05445: BWI76_RS05445 - type 11 methyltransferase, at 1,143,243 to 1,143,965
_RS05445
BWI76_RS05450: BWI76_RS05450 - ribonuclease HI, at 1,143,962 to 1,144,429
_RS05450
BWI76_RS05455: BWI76_RS05455 - DNA polymerase III subunit epsilon, at 1,144,495 to 1,145,226
_RS05455
Position (kb)
1143
1144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1142.278 kb on - strand at 1142.305 kb on + strand at 1142.384 kb on - strand at 1142.384 kb on - strand at 1142.453 kb on - strand at 1142.469 kb on + strand at 1142.491 kb on + strand at 1142.491 kb on + strand at 1142.499 kb on - strand at 1142.499 kb on - strand at 1142.637 kb on - strand, within BWI76_RS05440 at 1142.692 kb on + strand, within BWI76_RS05440 at 1142.715 kb on + strand, within BWI76_RS05440 at 1142.718 kb on - strand, within BWI76_RS05440 at 1142.736 kb on - strand, within BWI76_RS05440 at 1142.750 kb on + strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.800 kb on - strand, within BWI76_RS05440 at 1142.841 kb on + strand, within BWI76_RS05440 at 1142.879 kb on - strand, within BWI76_RS05440 at 1142.879 kb on - strand, within BWI76_RS05440 at 1143.029 kb on + strand, within BWI76_RS05440 at 1143.076 kb on - strand, within BWI76_RS05440 at 1143.264 kb on - strand at 1143.294 kb on + strand at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.352 kb on - strand, within BWI76_RS05445 at 1143.470 kb on + strand, within BWI76_RS05445 at 1143.470 kb on + strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.478 kb on - strand, within BWI76_RS05445 at 1143.622 kb on + strand, within BWI76_RS05445 at 1143.641 kb on + strand, within BWI76_RS05445 at 1144.210 kb on + strand, within BWI76_RS05450 at 1144.317 kb on - strand, within BWI76_RS05450 at 1144.492 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 1,142,278 - -0.6 1,142,305 + -0.1 1,142,384 - -0.8 1,142,384 - -0.7 1,142,453 - -0.4 1,142,469 + -0.8 1,142,491 + -1.7 1,142,491 + -2.4 1,142,499 - -0.3 1,142,499 - -0.8 1,142,637 - BWI76_RS05440 0.24 -0.6 1,142,692 + BWI76_RS05440 0.31 -0.4 1,142,715 + BWI76_RS05440 0.34 +0.0 1,142,718 - BWI76_RS05440 0.35 -0.4 1,142,736 - BWI76_RS05440 0.37 -0.9 1,142,750 + BWI76_RS05440 0.39 -0.7 1,142,800 - BWI76_RS05440 0.46 +0.1 1,142,800 - BWI76_RS05440 0.46 -0.4 1,142,800 - BWI76_RS05440 0.46 -1.8 1,142,841 + BWI76_RS05440 0.51 +2.3 1,142,879 - BWI76_RS05440 0.56 -0.6 1,142,879 - BWI76_RS05440 0.56 +0.5 1,143,029 + BWI76_RS05440 0.76 -2.4 1,143,076 - BWI76_RS05440 0.82 +0.3 1,143,264 - -0.6 1,143,294 + +2.1 1,143,352 - BWI76_RS05445 0.15 -1.1 1,143,352 - BWI76_RS05445 0.15 -2.8 1,143,470 + BWI76_RS05445 0.31 -0.8 1,143,470 + BWI76_RS05445 0.31 -0.6 1,143,478 - BWI76_RS05445 0.33 +0.7 1,143,478 - BWI76_RS05445 0.33 -1.7 1,143,478 - BWI76_RS05445 0.33 -1.9 1,143,478 - BWI76_RS05445 0.33 +0.2 1,143,622 + BWI76_RS05445 0.52 -2.1 1,143,641 + BWI76_RS05445 0.55 +1.0 1,144,210 + BWI76_RS05450 0.53 -1.5 1,144,317 - BWI76_RS05450 0.76 +0.2 1,144,492 + -0.8
Or see this region's nucleotide sequence