Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05360

Experiment: Paraquat dichloride 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05350 and BWI76_RS05355 are separated by 108 nucleotidesBWI76_RS05355 and BWI76_RS05360 are separated by 39 nucleotidesBWI76_RS05360 and BWI76_RS05365 overlap by 8 nucleotides BWI76_RS05350: BWI76_RS05350 - Rcs stress response system protein RcsF, at 1,126,633 to 1,127,040 _RS05350 BWI76_RS05355: BWI76_RS05355 - methionine ABC transporter substrate-binding protein MetQ, at 1,127,149 to 1,127,964 _RS05355 BWI76_RS05360: BWI76_RS05360 - methionine ABC transporter permease, at 1,128,004 to 1,128,657 _RS05360 BWI76_RS05365: BWI76_RS05365 - D-methionine ABC transporter, ATP-binding protein, at 1,128,650 to 1,129,681 _RS05365 Position (kb) 1128 1129Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1127.008 kb on - strandat 1127.138 kb on - strandat 1127.155 kb on + strandat 1127.163 kb on - strandat 1127.214 kb on + strandat 1127.221 kb on - strandat 1127.301 kb on - strand, within BWI76_RS05355at 1127.301 kb on - strand, within BWI76_RS05355at 1127.302 kb on - strand, within BWI76_RS05355at 1127.542 kb on + strand, within BWI76_RS05355at 1127.690 kb on + strand, within BWI76_RS05355at 1127.693 kb on - strand, within BWI76_RS05355at 1127.698 kb on - strand, within BWI76_RS05355at 1127.698 kb on - strand, within BWI76_RS05355at 1127.892 kb on + strandat 1127.945 kb on - strandat 1128.075 kb on + strand, within BWI76_RS05360at 1128.081 kb on + strand, within BWI76_RS05360at 1128.089 kb on + strand, within BWI76_RS05360at 1128.111 kb on + strand, within BWI76_RS05360at 1128.206 kb on - strand, within BWI76_RS05360at 1128.442 kb on - strand, within BWI76_RS05360at 1128.442 kb on - strand, within BWI76_RS05360at 1128.691 kb on - strandat 1128.753 kb on - strandat 1128.753 kb on - strandat 1128.898 kb on - strand, within BWI76_RS05365at 1129.073 kb on - strand, within BWI76_RS05365at 1129.128 kb on + strand, within BWI76_RS05365at 1129.283 kb on - strand, within BWI76_RS05365at 1129.296 kb on - strand, within BWI76_RS05365at 1129.413 kb on - strand, within BWI76_RS05365at 1129.492 kb on + strand, within BWI76_RS05365at 1129.492 kb on + strand, within BWI76_RS05365at 1129.492 kb on + strand, within BWI76_RS05365at 1129.497 kb on + strand, within BWI76_RS05365at 1129.500 kb on - strand, within BWI76_RS05365at 1129.502 kb on + strand, within BWI76_RS05365at 1129.502 kb on + strand, within BWI76_RS05365at 1129.510 kb on - strand, within BWI76_RS05365at 1129.525 kb on + strand, within BWI76_RS05365at 1129.525 kb on + strand, within BWI76_RS05365at 1129.525 kb on + strand, within BWI76_RS05365at 1129.533 kb on - strand, within BWI76_RS05365at 1129.555 kb on - strand, within BWI76_RS05365

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Per-strain Table

Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml
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1,127,008 - +0.8
1,127,138 - -0.4
1,127,155 + -0.1
1,127,163 - +0.3
1,127,214 + +0.2
1,127,221 - -0.2
1,127,301 - BWI76_RS05355 0.19 -1.0
1,127,301 - BWI76_RS05355 0.19 +0.3
1,127,302 - BWI76_RS05355 0.19 -1.6
1,127,542 + BWI76_RS05355 0.48 -1.0
1,127,690 + BWI76_RS05355 0.66 -1.3
1,127,693 - BWI76_RS05355 0.67 -0.0
1,127,698 - BWI76_RS05355 0.67 -1.1
1,127,698 - BWI76_RS05355 0.67 -0.3
1,127,892 + -1.1
1,127,945 - -1.5
1,128,075 + BWI76_RS05360 0.11 -0.4
1,128,081 + BWI76_RS05360 0.12 -1.7
1,128,089 + BWI76_RS05360 0.13 -1.8
1,128,111 + BWI76_RS05360 0.16 -1.2
1,128,206 - BWI76_RS05360 0.31 -0.4
1,128,442 - BWI76_RS05360 0.67 +0.3
1,128,442 - BWI76_RS05360 0.67 -1.0
1,128,691 - -0.8
1,128,753 - -0.1
1,128,753 - -0.8
1,128,898 - BWI76_RS05365 0.24 +0.0
1,129,073 - BWI76_RS05365 0.41 -0.5
1,129,128 + BWI76_RS05365 0.46 -2.6
1,129,283 - BWI76_RS05365 0.61 -0.3
1,129,296 - BWI76_RS05365 0.63 +0.3
1,129,413 - BWI76_RS05365 0.74 -0.6
1,129,492 + BWI76_RS05365 0.82 -0.3
1,129,492 + BWI76_RS05365 0.82 +0.4
1,129,492 + BWI76_RS05365 0.82 +1.1
1,129,497 + BWI76_RS05365 0.82 -0.8
1,129,500 - BWI76_RS05365 0.82 -0.3
1,129,502 + BWI76_RS05365 0.83 -1.2
1,129,502 + BWI76_RS05365 0.83 +0.3
1,129,510 - BWI76_RS05365 0.83 -0.5
1,129,525 + BWI76_RS05365 0.85 +2.2
1,129,525 + BWI76_RS05365 0.85 -3.2
1,129,525 + BWI76_RS05365 0.85 -0.3
1,129,533 - BWI76_RS05365 0.86 -1.3
1,129,555 - BWI76_RS05365 0.88 -0.3

Or see this region's nucleotide sequence