Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02735 and BWI76_RS02740 overlap by 11 nucleotides BWI76_RS02740 and BWI76_RS02745 are separated by 322 nucleotides BWI76_RS02745 and BWI76_RS02750 are separated by 67 nucleotides BWI76_RS02750 and BWI76_RS02755 are separated by 30 nucleotides
BWI76_RS02735: BWI76_RS02735 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 554,590 to 555,333
_RS02735
BWI76_RS02740: BWI76_RS02740 - putative transcriptional regulator, at 555,323 to 555,880
_RS02740
BWI76_RS02745: BWI76_RS02745 - hypothetical protein, at 556,203 to 556,409
_RS02745
BWI76_RS02750: BWI76_RS02750 - DNA-binding transcriptional regulator AraC, at 556,477 to 557,349
_RS02750
BWI76_RS02755: BWI76_RS02755 - beta-galactosidase, at 557,380 to 559,389
_RS02755
Position (kb) 556
557 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 555.207 kb on - strand, within BWI76_RS02735 at 555.478 kb on - strand, within BWI76_RS02740 at 555.568 kb on + strand, within BWI76_RS02740 at 555.634 kb on - strand, within BWI76_RS02740 at 555.683 kb on + strand, within BWI76_RS02740 at 555.683 kb on + strand, within BWI76_RS02740 at 555.763 kb on + strand, within BWI76_RS02740 at 555.775 kb on + strand, within BWI76_RS02740 at 555.798 kb on - strand, within BWI76_RS02740 at 555.874 kb on + strand at 555.882 kb on - strand at 555.883 kb on - strand at 556.228 kb on - strand, within BWI76_RS02745 at 556.434 kb on + strand at 556.574 kb on + strand, within BWI76_RS02750 at 556.790 kb on + strand, within BWI76_RS02750 at 556.844 kb on - strand, within BWI76_RS02750 at 557.024 kb on - strand, within BWI76_RS02750 at 557.068 kb on - strand, within BWI76_RS02750 at 557.142 kb on + strand, within BWI76_RS02750 at 557.145 kb on - strand, within BWI76_RS02750 at 557.236 kb on + strand, within BWI76_RS02750 at 557.244 kb on - strand, within BWI76_RS02750
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 555,207 - BWI76_RS02735 0.83 +0.2 555,478 - BWI76_RS02740 0.28 +2.7 555,568 + BWI76_RS02740 0.44 -0.5 555,634 - BWI76_RS02740 0.56 +0.4 555,683 + BWI76_RS02740 0.65 +0.9 555,683 + BWI76_RS02740 0.65 -0.0 555,763 + BWI76_RS02740 0.79 -0.3 555,775 + BWI76_RS02740 0.81 +0.3 555,798 - BWI76_RS02740 0.85 -2.8 555,874 + +0.0 555,882 - +3.2 555,883 - -0.5 556,228 - BWI76_RS02745 0.12 +0.6 556,434 + -1.3 556,574 + BWI76_RS02750 0.11 +1.1 556,790 + BWI76_RS02750 0.36 +0.2 556,844 - BWI76_RS02750 0.42 +1.0 557,024 - BWI76_RS02750 0.63 +0.1 557,068 - BWI76_RS02750 0.68 +0.3 557,142 + BWI76_RS02750 0.76 +0.3 557,145 - BWI76_RS02750 0.77 -0.9 557,236 + BWI76_RS02750 0.87 -0.7 557,244 - BWI76_RS02750 0.88 +0.1
Or see this region's nucleotide sequence