Experiment: Paraquat dichloride 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01980 and BWI76_RS01985 are separated by 145 nucleotides BWI76_RS01985 and BWI76_RS01990 are separated by 544 nucleotides BWI76_RS01990 and BWI76_RS01995 overlap by 16 nucleotides BWI76_RS01995 and BWI76_RS02000 are separated by 84 nucleotides
BWI76_RS01980: BWI76_RS01980 - anti-adapter protein IraM, at 407,774 to 408,163
_RS01980
BWI76_RS01985: BWI76_RS01985 - membrane protein, at 408,309 to 408,590
_RS01985
BWI76_RS01990: BWI76_RS01990 - cyclic diguanylate phosphodiesterase, at 409,135 to 410,739
_RS01990
BWI76_RS01995: BWI76_RS01995 - AraC family transcriptional regulator, at 410,724 to 411,053
_RS01995
BWI76_RS02000: BWI76_RS02000 - transcriptional regulator, at 411,138 to 411,596
_RS02000
Position (kb)
409
410
411 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 408.166 kb on - strand at 408.200 kb on - strand at 408.208 kb on + strand at 408.208 kb on + strand at 408.247 kb on + strand at 408.255 kb on - strand at 408.255 kb on - strand at 408.265 kb on - strand at 408.283 kb on - strand at 408.394 kb on + strand, within BWI76_RS01985 at 408.449 kb on + strand, within BWI76_RS01985 at 408.449 kb on + strand, within BWI76_RS01985 at 408.457 kb on - strand, within BWI76_RS01985 at 408.457 kb on - strand, within BWI76_RS01985 at 408.470 kb on + strand, within BWI76_RS01985 at 408.498 kb on - strand at 408.528 kb on + strand, within BWI76_RS01985 at 408.528 kb on + strand, within BWI76_RS01985 at 408.528 kb on + strand, within BWI76_RS01985 at 408.541 kb on - strand, within BWI76_RS01985 at 408.546 kb on - strand, within BWI76_RS01985 at 408.553 kb on - strand, within BWI76_RS01985 at 408.563 kb on - strand at 408.592 kb on + strand at 408.654 kb on + strand at 408.772 kb on - strand at 408.880 kb on + strand at 408.939 kb on + strand at 409.009 kb on - strand at 409.030 kb on + strand at 409.035 kb on + strand at 409.038 kb on - strand at 409.043 kb on - strand at 409.049 kb on + strand at 409.049 kb on + strand at 409.062 kb on - strand at 409.062 kb on - strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.088 kb on + strand at 409.096 kb on - strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.102 kb on + strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.110 kb on - strand at 409.137 kb on + strand at 409.180 kb on + strand at 409.180 kb on + strand at 409.184 kb on + strand at 409.194 kb on - strand at 409.204 kb on + strand at 409.217 kb on + strand at 409.222 kb on + strand at 409.235 kb on - strand at 409.329 kb on - strand, within BWI76_RS01990 at 409.374 kb on + strand, within BWI76_RS01990 at 409.381 kb on - strand, within BWI76_RS01990 at 409.404 kb on + strand, within BWI76_RS01990 at 409.412 kb on - strand, within BWI76_RS01990 at 409.412 kb on - strand, within BWI76_RS01990 at 409.417 kb on + strand, within BWI76_RS01990 at 409.571 kb on + strand, within BWI76_RS01990 at 409.571 kb on + strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.579 kb on - strand, within BWI76_RS01990 at 409.612 kb on + strand, within BWI76_RS01990 at 409.614 kb on + strand, within BWI76_RS01990 at 409.616 kb on + strand, within BWI76_RS01990 at 409.620 kb on - strand, within BWI76_RS01990 at 409.622 kb on - strand, within BWI76_RS01990 at 409.717 kb on + strand, within BWI76_RS01990 at 409.720 kb on - strand, within BWI76_RS01990 at 409.725 kb on - strand, within BWI76_RS01990 at 409.726 kb on + strand, within BWI76_RS01990 at 409.734 kb on - strand, within BWI76_RS01990 at 409.824 kb on - strand, within BWI76_RS01990 at 409.864 kb on + strand, within BWI76_RS01990 at 409.864 kb on + strand, within BWI76_RS01990 at 409.864 kb on + strand, within BWI76_RS01990 at 409.865 kb on + strand, within BWI76_RS01990 at 409.872 kb on - strand, within BWI76_RS01990 at 409.887 kb on + strand, within BWI76_RS01990 at 409.887 kb on + strand, within BWI76_RS01990 at 409.933 kb on + strand, within BWI76_RS01990 at 409.945 kb on + strand, within BWI76_RS01990 at 409.974 kb on - strand, within BWI76_RS01990 at 409.997 kb on + strand, within BWI76_RS01990 at 410.141 kb on - strand, within BWI76_RS01990 at 410.142 kb on - strand, within BWI76_RS01990 at 410.279 kb on - strand, within BWI76_RS01990 at 410.291 kb on + strand, within BWI76_RS01990 at 410.413 kb on + strand, within BWI76_RS01990 at 410.422 kb on + strand, within BWI76_RS01990 at 410.430 kb on - strand, within BWI76_RS01990 at 410.625 kb on - strand at 410.915 kb on - strand, within BWI76_RS01995 at 411.091 kb on - strand at 411.314 kb on + strand, within BWI76_RS02000 at 411.385 kb on + strand, within BWI76_RS02000 at 411.393 kb on - strand, within BWI76_RS02000 at 411.393 kb on - strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.454 kb on + strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.462 kb on - strand, within BWI76_RS02000 at 411.699 kb on + strand at 411.699 kb on + strand at 411.699 kb on + strand at 411.699 kb on + strand at 411.702 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.707 kb on - strand at 411.727 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.1 mg/ml remove 408,166 - +0.7 408,200 - -0.2 408,208 + -0.3 408,208 + +0.5 408,247 + +1.3 408,255 - +0.1 408,255 - -0.3 408,265 - -1.2 408,283 - -0.4 408,394 + BWI76_RS01985 0.30 +0.9 408,449 + BWI76_RS01985 0.50 +0.5 408,449 + BWI76_RS01985 0.50 +0.1 408,457 - BWI76_RS01985 0.52 -0.5 408,457 - BWI76_RS01985 0.52 -0.2 408,470 + BWI76_RS01985 0.57 -0.1 408,498 - +0.6 408,528 + BWI76_RS01985 0.78 +0.6 408,528 + BWI76_RS01985 0.78 +0.3 408,528 + BWI76_RS01985 0.78 -0.3 408,541 - BWI76_RS01985 0.82 +0.0 408,546 - BWI76_RS01985 0.84 -0.7 408,553 - BWI76_RS01985 0.87 -0.1 408,563 - -3.2 408,592 + -0.5 408,654 + +0.2 408,772 - +0.6 408,880 + +1.2 408,939 + -0.3 409,009 - -1.4 409,030 + -0.3 409,035 + +0.0 409,038 - +2.2 409,043 - +0.1 409,049 + -0.1 409,049 + +0.3 409,062 - -0.5 409,062 - +0.3 409,088 + -0.2 409,088 + -1.0 409,088 + +0.3 409,088 + -0.6 409,096 - -0.3 409,102 + -1.8 409,102 + +0.8 409,102 + +0.4 409,102 + -1.1 409,110 - +1.9 409,110 - +0.4 409,110 - +0.8 409,110 - -0.1 409,110 - -0.7 409,137 + -0.8 409,180 + -0.6 409,180 + +0.2 409,184 + +1.0 409,194 - +0.2 409,204 + -1.3 409,217 + -0.7 409,222 + +0.1 409,235 - -0.1 409,329 - BWI76_RS01990 0.12 -0.1 409,374 + BWI76_RS01990 0.15 +1.8 409,381 - BWI76_RS01990 0.15 +0.6 409,404 + BWI76_RS01990 0.17 -0.7 409,412 - BWI76_RS01990 0.17 +0.9 409,412 - BWI76_RS01990 0.17 -0.9 409,417 + BWI76_RS01990 0.18 -0.5 409,571 + BWI76_RS01990 0.27 -0.9 409,571 + BWI76_RS01990 0.27 +0.9 409,579 - BWI76_RS01990 0.28 -1.0 409,579 - BWI76_RS01990 0.28 +0.5 409,579 - BWI76_RS01990 0.28 +0.0 409,579 - BWI76_RS01990 0.28 +0.6 409,579 - BWI76_RS01990 0.28 +0.3 409,612 + BWI76_RS01990 0.30 -0.3 409,614 + BWI76_RS01990 0.30 -1.2 409,616 + BWI76_RS01990 0.30 +0.7 409,620 - BWI76_RS01990 0.30 -0.2 409,622 - BWI76_RS01990 0.30 +0.2 409,717 + BWI76_RS01990 0.36 -1.7 409,720 - BWI76_RS01990 0.36 +0.0 409,725 - BWI76_RS01990 0.37 -1.0 409,726 + BWI76_RS01990 0.37 +1.4 409,734 - BWI76_RS01990 0.37 -0.5 409,824 - BWI76_RS01990 0.43 -2.6 409,864 + BWI76_RS01990 0.45 -0.1 409,864 + BWI76_RS01990 0.45 -0.4 409,864 + BWI76_RS01990 0.45 -0.5 409,865 + BWI76_RS01990 0.45 -0.5 409,872 - BWI76_RS01990 0.46 +0.3 409,887 + BWI76_RS01990 0.47 +0.4 409,887 + BWI76_RS01990 0.47 +1.9 409,933 + BWI76_RS01990 0.50 +0.9 409,945 + BWI76_RS01990 0.50 -0.3 409,974 - BWI76_RS01990 0.52 +0.7 409,997 + BWI76_RS01990 0.54 +1.6 410,141 - BWI76_RS01990 0.63 -0.0 410,142 - BWI76_RS01990 0.63 -0.4 410,279 - BWI76_RS01990 0.71 -0.9 410,291 + BWI76_RS01990 0.72 -0.4 410,413 + BWI76_RS01990 0.80 -0.0 410,422 + BWI76_RS01990 0.80 +0.7 410,430 - BWI76_RS01990 0.81 -0.3 410,625 - +0.4 410,915 - BWI76_RS01995 0.58 -1.9 411,091 - -1.6 411,314 + BWI76_RS02000 0.38 -1.8 411,385 + BWI76_RS02000 0.54 -1.4 411,393 - BWI76_RS02000 0.56 -3.7 411,393 - BWI76_RS02000 0.56 -1.5 411,454 + BWI76_RS02000 0.69 -1.3 411,454 + BWI76_RS02000 0.69 -1.2 411,454 + BWI76_RS02000 0.69 -0.2 411,454 + BWI76_RS02000 0.69 -2.0 411,454 + BWI76_RS02000 0.69 -1.8 411,462 - BWI76_RS02000 0.71 -2.0 411,462 - BWI76_RS02000 0.71 +0.9 411,462 - BWI76_RS02000 0.71 +1.1 411,462 - BWI76_RS02000 0.71 -1.2 411,699 + -0.3 411,699 + +0.9 411,699 + +0.2 411,699 + +1.9 411,702 - +0.9 411,707 - -0.9 411,707 - +1.3 411,707 - -1.0 411,707 - +0.4 411,727 + +0.3
Or see this region's nucleotide sequence