Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5318 and Pf6N2E2_5319 are separated by 3 nucleotides Pf6N2E2_5319 and Pf6N2E2_5320 are separated by 0 nucleotides Pf6N2E2_5320 and Pf6N2E2_5321 are separated by 11 nucleotides
Pf6N2E2_5318 - Cytochrome O ubiquinol oxidase subunit I (EC 1.10.3.-), at 6,113,325 to 6,115,355
_5318
Pf6N2E2_5319 - Cytochrome O ubiquinol oxidase subunit III (EC 1.10.3.-), at 6,115,359 to 6,115,985
_5319
Pf6N2E2_5320 - Cytochrome O ubiquinol oxidase subunit IV (EC 1.10.3.-), at 6,115,986 to 6,116,321
_5320
Pf6N2E2_5321 - Heme O synthase, protoheme IX farnesyltransferase (EC 2.5.1.-) COX10-CtaB, at 6,116,333 to 6,117,220
_5321
Position (kb)
6115
6116
6117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.068 kb on + strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.069 kb on - strand, within Pf6N2E2_5318 at 6115.200 kb on + strand at 6115.201 kb on - strand at 6115.236 kb on + strand at 6115.236 kb on + strand at 6115.237 kb on - strand at 6115.237 kb on - strand at 6115.353 kb on + strand at 6115.353 kb on + strand at 6115.353 kb on + strand at 6115.354 kb on - strand at 6115.369 kb on + strand at 6115.369 kb on + strand at 6115.370 kb on - strand at 6115.370 kb on - strand at 6115.464 kb on + strand, within Pf6N2E2_5319 at 6115.465 kb on - strand, within Pf6N2E2_5319 at 6115.509 kb on + strand, within Pf6N2E2_5319 at 6115.524 kb on - strand, within Pf6N2E2_5319 at 6115.572 kb on + strand, within Pf6N2E2_5319 at 6115.573 kb on - strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.576 kb on + strand, within Pf6N2E2_5319 at 6115.577 kb on - strand, within Pf6N2E2_5319 at 6115.577 kb on - strand, within Pf6N2E2_5319 at 6115.617 kb on + strand, within Pf6N2E2_5319 at 6115.617 kb on + strand, within Pf6N2E2_5319 at 6115.618 kb on - strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.854 kb on + strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.855 kb on - strand, within Pf6N2E2_5319 at 6115.965 kb on + strand at 6115.965 kb on + strand at 6115.965 kb on + strand at 6115.966 kb on - strand at 6115.966 kb on - strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.983 kb on + strand at 6115.984 kb on - strand at 6115.984 kb on - strand at 6115.984 kb on - strand at 6116.044 kb on - strand, within Pf6N2E2_5320 at 6116.103 kb on + strand, within Pf6N2E2_5320 at 6116.103 kb on + strand, within Pf6N2E2_5320 at 6116.104 kb on - strand, within Pf6N2E2_5320 at 6116.104 kb on - strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.164 kb on + strand, within Pf6N2E2_5320 at 6116.165 kb on - strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.181 kb on + strand, within Pf6N2E2_5320 at 6116.182 kb on - strand, within Pf6N2E2_5320 at 6116.182 kb on - strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.216 kb on + strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.217 kb on - strand, within Pf6N2E2_5320 at 6116.636 kb on + strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.691 kb on - strand, within Pf6N2E2_5321 at 6116.703 kb on - strand, within Pf6N2E2_5321 at 6116.703 kb on - strand, within Pf6N2E2_5321 at 6116.711 kb on + strand, within Pf6N2E2_5321 at 6116.784 kb on - strand, within Pf6N2E2_5321 at 6116.864 kb on + strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.865 kb on - strand, within Pf6N2E2_5321 at 6116.966 kb on + strand, within Pf6N2E2_5321 at 6116.966 kb on + strand, within Pf6N2E2_5321 at 6116.967 kb on - strand, within Pf6N2E2_5321 at 6116.967 kb on - strand, within Pf6N2E2_5321 at 6117.029 kb on + strand, within Pf6N2E2_5321 at 6117.029 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.059 kb on + strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.060 kb on - strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.068 kb on + strand, within Pf6N2E2_5321 at 6117.086 kb on + strand, within Pf6N2E2_5321 at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.209 kb on + strand at 6117.210 kb on - strand at 6117.210 kb on - strand at 6117.218 kb on + strand at 6117.218 kb on + strand at 6117.218 kb on + strand at 6117.219 kb on - strand at 6117.219 kb on - strand at 6117.219 kb on - strand at 6117.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 6,115,068 + Pf6N2E2_5318 0.86 +0.1 6,115,068 + Pf6N2E2_5318 0.86 +0.1 6,115,068 + Pf6N2E2_5318 0.86 -1.2 6,115,068 + Pf6N2E2_5318 0.86 -0.4 6,115,068 + Pf6N2E2_5318 0.86 -1.0 6,115,069 - Pf6N2E2_5318 0.86 -0.4 6,115,069 - Pf6N2E2_5318 0.86 -0.2 6,115,069 - Pf6N2E2_5318 0.86 -1.0 6,115,069 - Pf6N2E2_5318 0.86 +1.2 6,115,069 - Pf6N2E2_5318 0.86 +0.1 6,115,069 - Pf6N2E2_5318 0.86 -0.3 6,115,200 + +0.7 6,115,201 - +0.6 6,115,236 + -0.6 6,115,236 + -1.4 6,115,237 - -0.0 6,115,237 - +0.3 6,115,353 + -0.3 6,115,353 + -0.8 6,115,353 + -0.9 6,115,354 - -1.6 6,115,369 + -0.3 6,115,369 + -0.3 6,115,370 - -0.7 6,115,370 - +0.2 6,115,464 + Pf6N2E2_5319 0.17 -0.7 6,115,465 - Pf6N2E2_5319 0.17 +0.2 6,115,509 + Pf6N2E2_5319 0.24 -0.3 6,115,524 - Pf6N2E2_5319 0.26 -0.9 6,115,572 + Pf6N2E2_5319 0.34 +0.3 6,115,573 - Pf6N2E2_5319 0.34 -0.2 6,115,576 + Pf6N2E2_5319 0.35 -0.6 6,115,576 + Pf6N2E2_5319 0.35 +0.1 6,115,576 + Pf6N2E2_5319 0.35 -0.7 6,115,577 - Pf6N2E2_5319 0.35 -0.5 6,115,577 - Pf6N2E2_5319 0.35 -0.8 6,115,617 + Pf6N2E2_5319 0.41 -1.4 6,115,617 + Pf6N2E2_5319 0.41 -1.5 6,115,618 - Pf6N2E2_5319 0.41 +0.0 6,115,854 + Pf6N2E2_5319 0.79 -0.5 6,115,854 + Pf6N2E2_5319 0.79 -0.8 6,115,854 + Pf6N2E2_5319 0.79 -0.1 6,115,854 + Pf6N2E2_5319 0.79 -0.3 6,115,854 + Pf6N2E2_5319 0.79 -0.3 6,115,854 + Pf6N2E2_5319 0.79 -0.5 6,115,854 + Pf6N2E2_5319 0.79 -0.1 6,115,854 + Pf6N2E2_5319 0.79 +0.1 6,115,855 - Pf6N2E2_5319 0.79 -0.3 6,115,855 - Pf6N2E2_5319 0.79 -0.2 6,115,855 - Pf6N2E2_5319 0.79 -0.9 6,115,855 - Pf6N2E2_5319 0.79 -0.2 6,115,855 - Pf6N2E2_5319 0.79 -0.6 6,115,965 + -0.2 6,115,965 + -0.7 6,115,965 + +1.9 6,115,966 - -0.2 6,115,966 - -0.6 6,115,983 + -0.6 6,115,983 + -1.2 6,115,983 + +0.1 6,115,983 + -0.7 6,115,984 - -2.1 6,115,984 - -0.3 6,115,984 - +0.4 6,116,044 - Pf6N2E2_5320 0.17 -0.3 6,116,103 + Pf6N2E2_5320 0.35 +0.3 6,116,103 + Pf6N2E2_5320 0.35 -2.9 6,116,104 - Pf6N2E2_5320 0.35 -0.7 6,116,104 - Pf6N2E2_5320 0.35 -0.9 6,116,164 + Pf6N2E2_5320 0.53 -0.3 6,116,164 + Pf6N2E2_5320 0.53 +0.2 6,116,164 + Pf6N2E2_5320 0.53 -1.8 6,116,165 - Pf6N2E2_5320 0.53 -0.5 6,116,181 + Pf6N2E2_5320 0.58 -0.5 6,116,181 + Pf6N2E2_5320 0.58 +0.9 6,116,181 + Pf6N2E2_5320 0.58 -2.4 6,116,182 - Pf6N2E2_5320 0.58 -0.2 6,116,182 - Pf6N2E2_5320 0.58 -0.4 6,116,216 + Pf6N2E2_5320 0.68 -0.4 6,116,216 + Pf6N2E2_5320 0.68 -0.2 6,116,216 + Pf6N2E2_5320 0.68 -0.8 6,116,216 + Pf6N2E2_5320 0.68 -0.6 6,116,216 + Pf6N2E2_5320 0.68 -0.3 6,116,216 + Pf6N2E2_5320 0.68 -2.6 6,116,216 + Pf6N2E2_5320 0.68 -0.7 6,116,217 - Pf6N2E2_5320 0.69 -0.1 6,116,217 - Pf6N2E2_5320 0.69 -0.1 6,116,217 - Pf6N2E2_5320 0.69 -0.7 6,116,217 - Pf6N2E2_5320 0.69 -0.0 6,116,217 - Pf6N2E2_5320 0.69 -0.7 6,116,217 - Pf6N2E2_5320 0.69 -0.5 6,116,636 + Pf6N2E2_5321 0.34 +0.4 6,116,691 - Pf6N2E2_5321 0.40 -1.2 6,116,691 - Pf6N2E2_5321 0.40 -0.9 6,116,691 - Pf6N2E2_5321 0.40 -0.2 6,116,703 - Pf6N2E2_5321 0.42 -0.0 6,116,703 - Pf6N2E2_5321 0.42 +0.0 6,116,711 + Pf6N2E2_5321 0.43 -1.3 6,116,784 - Pf6N2E2_5321 0.51 +0.4 6,116,864 + Pf6N2E2_5321 0.60 -0.9 6,116,865 - Pf6N2E2_5321 0.60 -0.1 6,116,865 - Pf6N2E2_5321 0.60 +0.1 6,116,865 - Pf6N2E2_5321 0.60 -0.3 6,116,966 + Pf6N2E2_5321 0.71 +0.4 6,116,966 + Pf6N2E2_5321 0.71 +0.3 6,116,967 - Pf6N2E2_5321 0.71 +0.1 6,116,967 - Pf6N2E2_5321 0.71 -0.7 6,117,029 + Pf6N2E2_5321 0.78 -0.5 6,117,029 + Pf6N2E2_5321 0.78 -0.2 6,117,059 + Pf6N2E2_5321 0.82 +0.3 6,117,059 + Pf6N2E2_5321 0.82 -0.8 6,117,059 + Pf6N2E2_5321 0.82 -2.1 6,117,059 + Pf6N2E2_5321 0.82 -0.0 6,117,059 + Pf6N2E2_5321 0.82 +0.5 6,117,059 + Pf6N2E2_5321 0.82 -0.5 6,117,059 + Pf6N2E2_5321 0.82 -1.1 6,117,060 - Pf6N2E2_5321 0.82 -1.2 6,117,060 - Pf6N2E2_5321 0.82 -1.0 6,117,060 - Pf6N2E2_5321 0.82 -0.1 6,117,060 - Pf6N2E2_5321 0.82 +0.4 6,117,060 - Pf6N2E2_5321 0.82 +1.6 6,117,068 + Pf6N2E2_5321 0.83 -0.1 6,117,068 + Pf6N2E2_5321 0.83 -1.0 6,117,068 + Pf6N2E2_5321 0.83 -0.4 6,117,068 + Pf6N2E2_5321 0.83 -1.4 6,117,086 + Pf6N2E2_5321 0.85 -1.0 6,117,209 + +0.3 6,117,209 + +0.5 6,117,209 + +0.0 6,117,209 + -0.7 6,117,210 - -0.6 6,117,210 - -0.6 6,117,218 + +0.0 6,117,218 + +0.4 6,117,218 + +0.3 6,117,219 - +0.2 6,117,219 - -0.2 6,117,219 - +0.5 6,117,219 - +0.1
Or see this region's nucleotide sequence