Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_332 and Pf6N2E2_333 are separated by 17 nucleotides Pf6N2E2_333 and Pf6N2E2_334 are separated by 167 nucleotides Pf6N2E2_334 and Pf6N2E2_335 are separated by 100 nucleotides Pf6N2E2_335 and Pf6N2E2_336 overlap by 4 nucleotides
Pf6N2E2_332 - putative nucleoside-diphosphate-sugar epimerase, at 399,970 to 400,848
_332
Pf6N2E2_333 - hypothetical protein, at 400,866 to 401,549
_333
Pf6N2E2_334 - Transcriptional regulator, at 401,717 to 402,670
_334
Pf6N2E2_335 - Aldo-keto reductase, at 402,771 to 403,634
_335
Pf6N2E2_336 - hypothetical protein, at 403,631 to 404,413
_336
Position (kb) 401
402
403 Strain fitness (log2 ratio) -2
-1
0
1 at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.844 kb on + strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.845 kb on - strand at 400.854 kb on + strand at 400.854 kb on + strand at 401.047 kb on + strand, within Pf6N2E2_333 at 401.047 kb on + strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.048 kb on - strand, within Pf6N2E2_333 at 401.143 kb on + strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.144 kb on - strand, within Pf6N2E2_333 at 401.191 kb on + strand, within Pf6N2E2_333 at 401.191 kb on + strand, within Pf6N2E2_333 at 401.192 kb on - strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.274 kb on + strand, within Pf6N2E2_333 at 401.275 kb on - strand, within Pf6N2E2_333 at 401.438 kb on - strand, within Pf6N2E2_333 at 401.501 kb on - strand at 401.501 kb on - strand at 401.549 kb on + strand at 401.649 kb on + strand at 401.649 kb on + strand at 401.650 kb on - strand at 401.838 kb on + strand, within Pf6N2E2_334 at 401.841 kb on + strand, within Pf6N2E2_334 at 401.910 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.937 kb on + strand, within Pf6N2E2_334 at 401.938 kb on - strand, within Pf6N2E2_334 at 401.957 kb on + strand, within Pf6N2E2_334 at 401.958 kb on - strand, within Pf6N2E2_334 at 402.051 kb on + strand, within Pf6N2E2_334 at 402.051 kb on + strand, within Pf6N2E2_334 at 402.150 kb on + strand, within Pf6N2E2_334 at 402.150 kb on + strand, within Pf6N2E2_334 at 402.151 kb on - strand, within Pf6N2E2_334 at 402.211 kb on + strand, within Pf6N2E2_334 at 402.212 kb on - strand, within Pf6N2E2_334 at 402.337 kb on + strand, within Pf6N2E2_334 at 402.686 kb on - strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.733 kb on + strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.734 kb on - strand at 402.957 kb on + strand, within Pf6N2E2_335 at 403.006 kb on - strand, within Pf6N2E2_335 at 403.332 kb on + strand, within Pf6N2E2_335 at 403.332 kb on + strand, within Pf6N2E2_335 at 403.395 kb on + strand, within Pf6N2E2_335 at 403.395 kb on + strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.396 kb on - strand, within Pf6N2E2_335 at 403.633 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 400,844 + -0.7 400,844 + -0.7 400,844 + +0.5 400,844 + +0.3 400,844 + +0.3 400,845 - -0.2 400,845 - +0.4 400,845 - +0.4 400,845 - +0.0 400,845 - +0.9 400,854 + +0.7 400,854 + -0.5 401,047 + Pf6N2E2_333 0.26 -0.5 401,047 + Pf6N2E2_333 0.26 -0.1 401,048 - Pf6N2E2_333 0.27 +0.5 401,048 - Pf6N2E2_333 0.27 +1.0 401,048 - Pf6N2E2_333 0.27 +1.7 401,048 - Pf6N2E2_333 0.27 -0.1 401,143 + Pf6N2E2_333 0.40 +0.0 401,144 - Pf6N2E2_333 0.41 -0.8 401,144 - Pf6N2E2_333 0.41 -1.0 401,144 - Pf6N2E2_333 0.41 +0.7 401,191 + Pf6N2E2_333 0.48 -0.3 401,191 + Pf6N2E2_333 0.48 +0.6 401,192 - Pf6N2E2_333 0.48 -2.2 401,274 + Pf6N2E2_333 0.60 +0.4 401,274 + Pf6N2E2_333 0.60 +1.0 401,274 + Pf6N2E2_333 0.60 +0.2 401,275 - Pf6N2E2_333 0.60 +1.3 401,438 - Pf6N2E2_333 0.84 +0.6 401,501 - +0.3 401,501 - -0.2 401,549 + +1.0 401,649 + -0.2 401,649 + -0.3 401,650 - -0.1 401,838 + Pf6N2E2_334 0.13 +1.1 401,841 + Pf6N2E2_334 0.13 +0.2 401,910 + Pf6N2E2_334 0.20 -0.3 401,937 + Pf6N2E2_334 0.23 +0.9 401,937 + Pf6N2E2_334 0.23 -0.2 401,937 + Pf6N2E2_334 0.23 -0.5 401,938 - Pf6N2E2_334 0.23 +0.2 401,957 + Pf6N2E2_334 0.25 -0.1 401,958 - Pf6N2E2_334 0.25 -0.3 402,051 + Pf6N2E2_334 0.35 -1.0 402,051 + Pf6N2E2_334 0.35 -0.4 402,150 + Pf6N2E2_334 0.45 -0.8 402,150 + Pf6N2E2_334 0.45 -1.4 402,151 - Pf6N2E2_334 0.45 -0.6 402,211 + Pf6N2E2_334 0.52 +0.2 402,212 - Pf6N2E2_334 0.52 -0.1 402,337 + Pf6N2E2_334 0.65 -0.2 402,686 - +1.3 402,733 + -0.5 402,733 + -0.1 402,733 + -1.8 402,733 + -0.0 402,733 + +0.1 402,734 - +0.5 402,734 - -0.5 402,734 - +0.9 402,734 - +0.4 402,734 - +1.2 402,734 - -0.9 402,957 + Pf6N2E2_335 0.22 -0.2 403,006 - Pf6N2E2_335 0.27 +0.4 403,332 + Pf6N2E2_335 0.65 -0.3 403,332 + Pf6N2E2_335 0.65 -0.4 403,395 + Pf6N2E2_335 0.72 -0.2 403,395 + Pf6N2E2_335 0.72 -0.3 403,396 - Pf6N2E2_335 0.72 -0.8 403,396 - Pf6N2E2_335 0.72 +0.1 403,396 - Pf6N2E2_335 0.72 +0.4 403,633 - -0.5
Or see this region's nucleotide sequence