Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_288 and Pf6N2E2_289 are separated by 482 nucleotides
Pf6N2E2_288 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 355,657 to 357,882
_288
Pf6N2E2_289 - Isocitrate dehydrogenase [NADP] (EC 1.1.1.42), at 358,365 to 359,621
_289
Position (kb) 355
356
357
358 Strain fitness (log2 ratio) -2
-1
0
1
2 at 354.692 kb on - strand at 354.712 kb on - strand at 354.715 kb on - strand at 354.715 kb on - strand at 354.784 kb on - strand at 354.814 kb on + strand at 354.903 kb on - strand at 354.907 kb on - strand at 354.941 kb on + strand at 354.941 kb on + strand at 354.989 kb on + strand at 354.995 kb on + strand at 354.995 kb on + strand at 355.179 kb on + strand at 355.208 kb on + strand at 355.243 kb on + strand at 355.297 kb on - strand at 355.300 kb on + strand at 355.300 kb on + strand at 355.434 kb on + strand at 355.435 kb on - strand at 355.497 kb on + strand at 355.497 kb on + strand at 355.501 kb on + strand at 355.501 kb on + strand at 355.501 kb on + strand at 355.501 kb on + strand at 355.515 kb on + strand at 355.515 kb on + strand at 355.516 kb on - strand at 355.521 kb on + strand at 355.536 kb on + strand at 355.536 kb on + strand at 355.536 kb on + strand at 355.547 kb on + strand at 355.547 kb on + strand at 355.547 kb on + strand at 355.548 kb on - strand at 355.579 kb on + strand at 355.579 kb on + strand at 355.579 kb on + strand at 355.579 kb on + strand at 355.579 kb on + strand at 355.598 kb on + strand at 355.650 kb on + strand at 355.650 kb on + strand at 355.650 kb on + strand at 355.651 kb on - strand at 355.651 kb on - strand at 355.651 kb on - strand at 355.658 kb on + strand at 355.659 kb on - strand at 355.659 kb on - strand at 355.659 kb on - strand at 355.659 kb on - strand at 355.659 kb on - strand at 355.659 kb on - strand at 355.659 kb on - strand at 355.659 kb on - strand at 355.737 kb on - strand at 355.737 kb on - strand at 356.249 kb on - strand, within Pf6N2E2_288 at 356.369 kb on + strand, within Pf6N2E2_288 at 356.370 kb on - strand, within Pf6N2E2_288 at 356.624 kb on + strand, within Pf6N2E2_288 at 357.059 kb on + strand, within Pf6N2E2_288 at 357.235 kb on + strand, within Pf6N2E2_288 at 357.908 kb on - strand at 357.908 kb on - strand at 358.052 kb on + strand at 358.077 kb on - strand at 358.077 kb on - strand at 358.119 kb on - strand at 358.120 kb on + strand at 358.120 kb on + strand at 358.120 kb on + strand at 358.120 kb on + strand at 358.120 kb on + strand at 358.120 kb on + strand at 358.120 kb on + strand at 358.120 kb on + strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.121 kb on - strand at 358.124 kb on + strand at 358.124 kb on + strand at 358.124 kb on + strand at 358.124 kb on + strand at 358.125 kb on - strand at 358.125 kb on - strand at 358.125 kb on - strand at 358.125 kb on - strand at 358.125 kb on - strand at 358.125 kb on - strand at 358.125 kb on - strand at 358.125 kb on - strand at 358.125 kb on - strand at 358.128 kb on + strand at 358.128 kb on + strand at 358.128 kb on + strand at 358.128 kb on + strand at 358.128 kb on + strand at 358.129 kb on - strand at 358.129 kb on - strand at 358.129 kb on - strand at 358.129 kb on - strand at 358.129 kb on - strand at 358.129 kb on - strand at 358.153 kb on + strand at 358.207 kb on + strand at 358.207 kb on + strand at 358.248 kb on + strand at 358.249 kb on - strand at 358.291 kb on - strand at 358.291 kb on - strand at 358.433 kb on + strand at 358.433 kb on + strand at 358.434 kb on - strand at 358.434 kb on - strand at 358.434 kb on - strand at 358.434 kb on - strand at 358.434 kb on - strand at 358.540 kb on - strand, within Pf6N2E2_289 at 358.540 kb on - strand, within Pf6N2E2_289 at 358.575 kb on + strand, within Pf6N2E2_289 at 358.576 kb on - strand, within Pf6N2E2_289 at 358.602 kb on + strand, within Pf6N2E2_289 at 358.602 kb on + strand, within Pf6N2E2_289 at 358.653 kb on + strand, within Pf6N2E2_289 at 358.653 kb on + strand, within Pf6N2E2_289 at 358.743 kb on + strand, within Pf6N2E2_289 at 358.744 kb on - strand, within Pf6N2E2_289 at 358.744 kb on - strand, within Pf6N2E2_289 at 358.744 kb on - strand, within Pf6N2E2_289 at 358.744 kb on - strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.848 kb on + strand, within Pf6N2E2_289 at 358.849 kb on - strand, within Pf6N2E2_289 at 358.849 kb on - strand, within Pf6N2E2_289 at 358.849 kb on - strand, within Pf6N2E2_289 at 358.849 kb on - strand, within Pf6N2E2_289 at 358.849 kb on - strand, within Pf6N2E2_289 at 358.849 kb on - strand, within Pf6N2E2_289 at 358.849 kb on - strand, within Pf6N2E2_289
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 354,692 - +0.4 354,712 - -0.3 354,715 - +0.2 354,715 - +1.0 354,784 - +1.0 354,814 + -0.6 354,903 - -1.4 354,907 - -0.0 354,941 + +0.6 354,941 + +0.6 354,989 + +0.1 354,995 + -0.9 354,995 + +0.2 355,179 + +1.1 355,208 + -1.4 355,243 + +0.7 355,297 - +0.0 355,300 + -0.8 355,300 + -0.7 355,434 + -0.4 355,435 - +0.5 355,497 + +1.3 355,497 + +0.9 355,501 + +1.1 355,501 + -0.3 355,501 + +0.3 355,501 + -0.1 355,515 + -0.8 355,515 + -0.2 355,516 - -0.5 355,521 + -1.0 355,536 + +0.1 355,536 + -0.1 355,536 + +0.4 355,547 + +0.4 355,547 + +0.6 355,547 + +0.2 355,548 - -1.1 355,579 + -0.9 355,579 + -0.2 355,579 + -1.0 355,579 + +0.9 355,579 + +0.5 355,598 + +0.3 355,650 + -0.6 355,650 + -0.1 355,650 + +0.6 355,651 - +1.4 355,651 - -0.5 355,651 - -1.4 355,658 + +0.3 355,659 - -0.9 355,659 - -1.2 355,659 - +0.3 355,659 - +0.4 355,659 - +0.6 355,659 - +0.3 355,659 - +0.1 355,659 - -0.1 355,737 - +1.0 355,737 - +0.7 356,249 - Pf6N2E2_288 0.27 -0.3 356,369 + Pf6N2E2_288 0.32 -1.9 356,370 - Pf6N2E2_288 0.32 -0.1 356,624 + Pf6N2E2_288 0.43 -0.5 357,059 + Pf6N2E2_288 0.63 +0.1 357,235 + Pf6N2E2_288 0.71 +0.1 357,908 - +0.3 357,908 - +0.5 358,052 + +0.2 358,077 - +1.2 358,077 - +0.3 358,119 - +1.0 358,120 + +1.1 358,120 + -0.1 358,120 + -0.2 358,120 + +0.9 358,120 + +0.1 358,120 + +0.9 358,120 + +0.7 358,120 + +0.5 358,121 - -0.3 358,121 - +1.3 358,121 - +0.8 358,121 - -0.7 358,121 - -0.4 358,121 - -1.5 358,121 - -0.8 358,121 - +0.0 358,121 - -0.3 358,121 - +0.4 358,121 - +2.3 358,121 - -0.1 358,124 + +0.4 358,124 + +0.6 358,124 + +0.7 358,124 + -0.6 358,125 - -0.9 358,125 - +0.5 358,125 - +0.8 358,125 - +0.1 358,125 - -0.7 358,125 - +0.1 358,125 - +1.7 358,125 - +1.8 358,125 - +1.0 358,128 + +0.1 358,128 + -0.8 358,128 + -0.5 358,128 + -0.6 358,128 + +0.6 358,129 - +0.4 358,129 - +2.1 358,129 - +0.4 358,129 - -0.5 358,129 - -0.7 358,129 - +0.4 358,153 + -2.1 358,207 + -0.8 358,207 + +0.1 358,248 + +0.2 358,249 - -0.2 358,291 - -0.2 358,291 - +0.8 358,433 + +0.5 358,433 + -0.1 358,434 - -0.1 358,434 - +0.5 358,434 - +0.2 358,434 - +0.1 358,434 - +0.3 358,540 - Pf6N2E2_289 0.14 -0.2 358,540 - Pf6N2E2_289 0.14 +0.2 358,575 + Pf6N2E2_289 0.17 -0.1 358,576 - Pf6N2E2_289 0.17 +0.5 358,602 + Pf6N2E2_289 0.19 +0.3 358,602 + Pf6N2E2_289 0.19 -0.1 358,653 + Pf6N2E2_289 0.23 +0.7 358,653 + Pf6N2E2_289 0.23 -0.2 358,743 + Pf6N2E2_289 0.30 +0.3 358,744 - Pf6N2E2_289 0.30 +0.4 358,744 - Pf6N2E2_289 0.30 +0.0 358,744 - Pf6N2E2_289 0.30 +0.7 358,744 - Pf6N2E2_289 0.30 +0.4 358,848 + Pf6N2E2_289 0.38 +0.2 358,848 + Pf6N2E2_289 0.38 -0.7 358,848 + Pf6N2E2_289 0.38 -0.3 358,848 + Pf6N2E2_289 0.38 +0.9 358,848 + Pf6N2E2_289 0.38 +0.3 358,848 + Pf6N2E2_289 0.38 +1.2 358,848 + Pf6N2E2_289 0.38 +0.2 358,848 + Pf6N2E2_289 0.38 +0.1 358,848 + Pf6N2E2_289 0.38 +0.2 358,848 + Pf6N2E2_289 0.38 +0.1 358,848 + Pf6N2E2_289 0.38 +0.9 358,848 + Pf6N2E2_289 0.38 -0.7 358,849 - Pf6N2E2_289 0.39 +0.6 358,849 - Pf6N2E2_289 0.39 +0.1 358,849 - Pf6N2E2_289 0.39 -0.4 358,849 - Pf6N2E2_289 0.39 -1.4 358,849 - Pf6N2E2_289 0.39 +0.4 358,849 - Pf6N2E2_289 0.39 +0.3 358,849 - Pf6N2E2_289 0.39 -0.6
Or see this region's nucleotide sequence