Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2854 and Pf6N2E2_2855 are separated by 161 nucleotides Pf6N2E2_2855 and Pf6N2E2_2856 are separated by 67 nucleotides Pf6N2E2_2856 and Pf6N2E2_2857 are separated by 140 nucleotides
Pf6N2E2_2854 - RNA polymerase sigma-70 factor, ECF subfamily, at 3,346,367 to 3,347,602
_2854
Pf6N2E2_2855 - DGPFAETKE, at 3,347,764 to 3,348,108
_2855
Pf6N2E2_2856 - Ketosteroid isomerase homolog, at 3,348,176 to 3,348,610
_2856
Pf6N2E2_2857 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,348,751 to 3,349,236
_2857
Position (kb)
3347
3348
3349 Strain fitness (log2 ratio)
-2
-1
0
1 at 3347.031 kb on + strand, within Pf6N2E2_2854 at 3347.031 kb on + strand, within Pf6N2E2_2854 at 3347.031 kb on + strand, within Pf6N2E2_2854 at 3347.031 kb on + strand, within Pf6N2E2_2854 at 3347.031 kb on + strand, within Pf6N2E2_2854 at 3347.032 kb on - strand, within Pf6N2E2_2854 at 3347.032 kb on - strand, within Pf6N2E2_2854 at 3347.032 kb on - strand, within Pf6N2E2_2854 at 3347.032 kb on - strand, within Pf6N2E2_2854 at 3347.032 kb on - strand, within Pf6N2E2_2854 at 3347.091 kb on + strand, within Pf6N2E2_2854 at 3347.134 kb on + strand, within Pf6N2E2_2854 at 3347.563 kb on - strand at 3347.708 kb on - strand at 3347.754 kb on + strand at 3347.754 kb on + strand at 3347.754 kb on + strand at 3347.754 kb on + strand at 3347.755 kb on - strand at 3347.755 kb on - strand at 3347.755 kb on - strand at 3347.755 kb on - strand at 3347.758 kb on + strand at 3347.758 kb on + strand at 3347.874 kb on - strand, within Pf6N2E2_2855 at 3347.874 kb on - strand, within Pf6N2E2_2855 at 3347.874 kb on - strand, within Pf6N2E2_2855 at 3347.874 kb on - strand, within Pf6N2E2_2855 at 3348.023 kb on + strand, within Pf6N2E2_2855 at 3348.084 kb on + strand at 3348.084 kb on + strand at 3348.084 kb on + strand at 3348.085 kb on - strand at 3348.085 kb on - strand at 3348.085 kb on - strand at 3348.085 kb on - strand at 3348.086 kb on + strand at 3348.086 kb on + strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.087 kb on - strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.097 kb on + strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.098 kb on - strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.101 kb on + strand at 3348.102 kb on - strand at 3348.102 kb on - strand at 3348.102 kb on - strand at 3348.102 kb on - strand at 3348.137 kb on + strand at 3348.137 kb on + strand at 3348.137 kb on + strand at 3348.138 kb on - strand at 3348.138 kb on - strand at 3348.138 kb on - strand at 3348.138 kb on - strand at 3348.146 kb on + strand at 3348.146 kb on + strand at 3348.146 kb on + strand at 3348.147 kb on - strand at 3348.185 kb on + strand at 3348.185 kb on + strand at 3348.185 kb on + strand at 3348.186 kb on - strand at 3348.186 kb on - strand at 3348.337 kb on - strand, within Pf6N2E2_2856 at 3348.337 kb on - strand, within Pf6N2E2_2856 at 3348.513 kb on + strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.561 kb on + strand, within Pf6N2E2_2856 at 3348.562 kb on - strand, within Pf6N2E2_2856 at 3348.604 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.723 kb on - strand at 3349.042 kb on + strand, within Pf6N2E2_2857
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 3,347,031 + Pf6N2E2_2854 0.54 -1.3 3,347,031 + Pf6N2E2_2854 0.54 -0.6 3,347,031 + Pf6N2E2_2854 0.54 -0.2 3,347,031 + Pf6N2E2_2854 0.54 -0.4 3,347,031 + Pf6N2E2_2854 0.54 +0.3 3,347,032 - Pf6N2E2_2854 0.54 +0.7 3,347,032 - Pf6N2E2_2854 0.54 +0.2 3,347,032 - Pf6N2E2_2854 0.54 +0.0 3,347,032 - Pf6N2E2_2854 0.54 +1.0 3,347,032 - Pf6N2E2_2854 0.54 -0.2 3,347,091 + Pf6N2E2_2854 0.59 +0.8 3,347,134 + Pf6N2E2_2854 0.62 +0.9 3,347,563 - -0.2 3,347,708 - +0.0 3,347,754 + -1.3 3,347,754 + -0.3 3,347,754 + +0.0 3,347,754 + -0.4 3,347,755 - +0.9 3,347,755 - -0.3 3,347,755 - +0.3 3,347,755 - +0.1 3,347,758 + +0.7 3,347,758 + -0.4 3,347,874 - Pf6N2E2_2855 0.32 -1.0 3,347,874 - Pf6N2E2_2855 0.32 +0.1 3,347,874 - Pf6N2E2_2855 0.32 -1.0 3,347,874 - Pf6N2E2_2855 0.32 +0.1 3,348,023 + Pf6N2E2_2855 0.75 -1.5 3,348,084 + +0.9 3,348,084 + -0.6 3,348,084 + -0.8 3,348,085 - +0.3 3,348,085 - +0.3 3,348,085 - +0.2 3,348,085 - +1.0 3,348,086 + -0.1 3,348,086 + -0.4 3,348,087 - +0.2 3,348,087 - +0.4 3,348,087 - -0.2 3,348,087 - -0.7 3,348,087 - -0.1 3,348,087 - +0.3 3,348,087 - -0.6 3,348,087 - +0.1 3,348,087 - -0.1 3,348,097 + +0.1 3,348,097 + -1.5 3,348,097 + -0.2 3,348,097 + -0.5 3,348,097 + +1.4 3,348,097 + +0.6 3,348,097 + +0.1 3,348,098 - -0.3 3,348,098 - -1.0 3,348,098 - +0.3 3,348,098 - +0.1 3,348,098 - -1.0 3,348,098 - -1.4 3,348,098 - -0.7 3,348,098 - +0.0 3,348,098 - +0.9 3,348,098 - -0.0 3,348,098 - +0.9 3,348,098 - +0.3 3,348,098 - +0.5 3,348,098 - -0.2 3,348,098 - +0.4 3,348,098 - -1.4 3,348,101 + +0.5 3,348,101 + -0.7 3,348,101 + +0.3 3,348,101 + +0.6 3,348,101 + -0.9 3,348,101 + +0.1 3,348,102 - +1.3 3,348,102 - -0.0 3,348,102 - +0.8 3,348,102 - +0.6 3,348,137 + -0.5 3,348,137 + -0.3 3,348,137 + +1.2 3,348,138 - +0.1 3,348,138 - -0.1 3,348,138 - -0.6 3,348,138 - +0.6 3,348,146 + +0.2 3,348,146 + -0.5 3,348,146 + -0.4 3,348,147 - +0.8 3,348,185 + +0.0 3,348,185 + +1.0 3,348,185 + +1.2 3,348,186 - -0.5 3,348,186 - +0.2 3,348,337 - Pf6N2E2_2856 0.37 -0.1 3,348,337 - Pf6N2E2_2856 0.37 +0.2 3,348,513 + Pf6N2E2_2856 0.77 -0.1 3,348,514 - Pf6N2E2_2856 0.78 -0.7 3,348,514 - Pf6N2E2_2856 0.78 +0.2 3,348,514 - Pf6N2E2_2856 0.78 +0.4 3,348,514 - Pf6N2E2_2856 0.78 +1.4 3,348,514 - Pf6N2E2_2856 0.78 +0.7 3,348,561 + Pf6N2E2_2856 0.89 +0.6 3,348,562 - Pf6N2E2_2856 0.89 +0.7 3,348,604 + -0.2 3,348,722 + +0.7 3,348,722 + +0.0 3,348,722 + -0.3 3,348,722 + +0.8 3,348,722 + +0.0 3,348,723 - +0.8 3,349,042 + Pf6N2E2_2857 0.60 -1.8
Or see this region's nucleotide sequence