Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_236 and Pf6N2E2_237 are separated by 291 nucleotides Pf6N2E2_237 and Pf6N2E2_238 are separated by 219 nucleotides Pf6N2E2_238 and Pf6N2E2_239 are separated by 33 nucleotides Pf6N2E2_239 and Pf6N2E2_240 are separated by 171 nucleotides
Pf6N2E2_236 - putative transcriptional regulator protein, at 298,600 to 299,253
_236
Pf6N2E2_237 - hypothetical protein, at 299,545 to 299,757
_237
Pf6N2E2_238 - hypothetical protein, at 299,977 to 300,096
_238
Pf6N2E2_239 - Fatty acid desaturase, at 300,130 to 300,831
_239
Pf6N2E2_240 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 301,003 to 301,893
_240
Position (kb) 299
300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 299.089 kb on + strand, within Pf6N2E2_236 at 299.090 kb on - strand, within Pf6N2E2_236 at 299.116 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.129 kb on + strand, within Pf6N2E2_236 at 299.130 kb on - strand, within Pf6N2E2_236 at 299.130 kb on - strand, within Pf6N2E2_236 at 299.165 kb on + strand, within Pf6N2E2_236 at 299.272 kb on + strand at 299.272 kb on + strand at 299.301 kb on - strand at 299.307 kb on + strand at 299.392 kb on + strand at 299.393 kb on - strand at 299.413 kb on + strand at 299.414 kb on - strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.491 kb on + strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.492 kb on - strand at 299.538 kb on + strand at 299.539 kb on - strand at 299.539 kb on - strand at 299.539 kb on - strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.544 kb on + strand at 299.545 kb on - strand at 299.545 kb on - strand at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.591 kb on - strand, within Pf6N2E2_237 at 299.747 kb on - strand at 299.786 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.810 kb on - strand at 299.813 kb on - strand at 299.813 kb on - strand at 299.871 kb on - strand at 299.880 kb on + strand at 299.880 kb on + strand at 299.880 kb on + strand at 299.881 kb on - strand at 299.881 kb on - strand at 299.881 kb on - strand at 299.896 kb on + strand at 299.978 kb on + strand at 299.979 kb on - strand at 299.992 kb on + strand, within Pf6N2E2_238 at 299.992 kb on + strand, within Pf6N2E2_238 at 300.010 kb on - strand, within Pf6N2E2_238 at 300.172 kb on + strand at 300.183 kb on + strand at 300.184 kb on - strand at 300.184 kb on - strand at 300.295 kb on + strand, within Pf6N2E2_239 at 300.295 kb on + strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.296 kb on - strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.297 kb on + strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.298 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.482 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.506 kb on - strand, within Pf6N2E2_239 at 300.559 kb on + strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.595 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.707 kb on - strand, within Pf6N2E2_239 at 300.709 kb on - strand, within Pf6N2E2_239 at 300.749 kb on - strand, within Pf6N2E2_239 at 300.764 kb on - strand at 300.764 kb on - strand at 300.773 kb on + strand at 300.830 kb on - strand at 300.835 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.844 kb on + strand at 300.845 kb on - strand at 300.845 kb on - strand at 300.894 kb on + strand at 300.894 kb on + strand at 300.960 kb on - strand at 300.960 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 299,089 + Pf6N2E2_236 0.75 +0.6 299,090 - Pf6N2E2_236 0.75 -0.4 299,116 + Pf6N2E2_236 0.79 +0.2 299,129 + Pf6N2E2_236 0.81 -0.2 299,129 + Pf6N2E2_236 0.81 -0.3 299,129 + Pf6N2E2_236 0.81 -1.0 299,130 - Pf6N2E2_236 0.81 -0.1 299,130 - Pf6N2E2_236 0.81 +0.1 299,165 + Pf6N2E2_236 0.86 +0.8 299,272 + -0.5 299,272 + +0.2 299,301 - +0.7 299,307 + +0.2 299,392 + -0.9 299,393 - +0.2 299,413 + +0.3 299,414 - +0.2 299,491 + +0.1 299,491 + +0.6 299,491 + -1.8 299,492 - -1.3 299,492 - -0.7 299,492 - -1.5 299,492 - -0.1 299,492 - -0.1 299,492 - +0.2 299,492 - -0.6 299,492 - +0.5 299,492 - +0.6 299,538 + +0.4 299,539 - -0.2 299,539 - -0.3 299,539 - +0.3 299,544 + +1.7 299,544 + +0.5 299,544 + +0.3 299,544 + -1.1 299,545 - -0.4 299,545 - -0.3 299,591 - Pf6N2E2_237 0.22 -0.5 299,591 - Pf6N2E2_237 0.22 -0.2 299,591 - Pf6N2E2_237 0.22 +0.3 299,591 - Pf6N2E2_237 0.22 -0.8 299,747 - -0.4 299,786 - +0.1 299,810 - +0.3 299,810 - +0.0 299,810 - -1.2 299,810 - +0.5 299,810 - -0.4 299,813 - -0.2 299,813 - +0.3 299,871 - -0.0 299,880 + +0.2 299,880 + +0.7 299,880 + -0.2 299,881 - -1.7 299,881 - -0.6 299,881 - -0.8 299,896 + +0.6 299,978 + +0.4 299,979 - -1.4 299,992 + Pf6N2E2_238 0.12 -0.3 299,992 + Pf6N2E2_238 0.12 -0.8 300,010 - Pf6N2E2_238 0.28 -1.7 300,172 + -0.0 300,183 + -0.0 300,184 - +0.5 300,184 - +0.3 300,295 + Pf6N2E2_239 0.24 +0.5 300,295 + Pf6N2E2_239 0.24 -1.3 300,296 - Pf6N2E2_239 0.24 +0.3 300,296 - Pf6N2E2_239 0.24 +1.4 300,297 + Pf6N2E2_239 0.24 +0.6 300,297 + Pf6N2E2_239 0.24 +0.6 300,297 + Pf6N2E2_239 0.24 +0.1 300,298 - Pf6N2E2_239 0.24 -0.2 300,298 - Pf6N2E2_239 0.24 -0.5 300,298 - Pf6N2E2_239 0.24 +0.3 300,298 - Pf6N2E2_239 0.24 +0.1 300,298 - Pf6N2E2_239 0.24 -0.1 300,298 - Pf6N2E2_239 0.24 -0.1 300,298 - Pf6N2E2_239 0.24 -1.1 300,482 - Pf6N2E2_239 0.50 +0.1 300,482 - Pf6N2E2_239 0.50 +0.4 300,506 - Pf6N2E2_239 0.54 -1.2 300,506 - Pf6N2E2_239 0.54 -0.5 300,559 + Pf6N2E2_239 0.61 +0.1 300,595 - Pf6N2E2_239 0.66 +0.7 300,595 - Pf6N2E2_239 0.66 +0.7 300,707 - Pf6N2E2_239 0.82 -3.1 300,707 - Pf6N2E2_239 0.82 -1.1 300,709 - Pf6N2E2_239 0.82 -0.2 300,749 - Pf6N2E2_239 0.88 +0.5 300,764 - -0.4 300,764 - +0.2 300,773 + +0.0 300,830 - +0.0 300,835 + +0.1 300,844 + -1.1 300,844 + +0.4 300,844 + -0.5 300,844 + -0.6 300,845 - +0.3 300,845 - +0.6 300,894 + -0.2 300,894 + +0.2 300,960 - +0.4 300,960 - +0.5
Or see this region's nucleotide sequence