Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2109 and Pf6N2E2_2110 are separated by 29 nucleotides Pf6N2E2_2110 and Pf6N2E2_2111 are separated by 168 nucleotides Pf6N2E2_2111 and Pf6N2E2_2112 are separated by 35 nucleotides
Pf6N2E2_2109 - NAD-dependent protein deacetylase of SIR2 family, at 2,506,171 to 2,507,010
_2109
Pf6N2E2_2110 - LysR family transcriptional regulator near succinyl-CoA:3-ketoacid-coenzyme A transferase, at 2,507,040 to 2,507,942
_2110
Pf6N2E2_2111 - Dehydrocarnitine CoA-transferase and acetoacetate CoA-transferase, subunit A (from data) , at 2,508,111 to 2,508,809
_2111
Pf6N2E2_2112 - Dehydrocarnitine CoA-transferase and acetoacetate CoA-transferase, subunit B (from data) , at 2,508,845 to 2,509,510
_2112
Position (kb)
2507
2508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.046 kb on + strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.047 kb on - strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.216 kb on + strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.217 kb on - strand at 2506.330 kb on + strand, within Pf6N2E2_2109 at 2506.330 kb on + strand, within Pf6N2E2_2109 at 2506.330 kb on + strand, within Pf6N2E2_2109 at 2506.331 kb on - strand, within Pf6N2E2_2109 at 2506.331 kb on - strand, within Pf6N2E2_2109 at 2506.331 kb on - strand, within Pf6N2E2_2109 at 2506.855 kb on + strand, within Pf6N2E2_2109 at 2506.856 kb on - strand, within Pf6N2E2_2109 at 2506.856 kb on - strand, within Pf6N2E2_2109 at 2507.008 kb on + strand at 2507.008 kb on + strand at 2507.009 kb on - strand at 2507.009 kb on - strand at 2507.461 kb on + strand, within Pf6N2E2_2110 at 2507.461 kb on + strand, within Pf6N2E2_2110 at 2507.462 kb on - strand, within Pf6N2E2_2110 at 2507.462 kb on - strand, within Pf6N2E2_2110 at 2507.462 kb on - strand, within Pf6N2E2_2110 at 2507.792 kb on + strand, within Pf6N2E2_2110 at 2507.792 kb on + strand, within Pf6N2E2_2110 at 2507.793 kb on - strand, within Pf6N2E2_2110 at 2507.972 kb on + strand at 2507.973 kb on - strand at 2508.097 kb on + strand at 2508.097 kb on + strand at 2508.098 kb on - strand at 2508.098 kb on - strand at 2508.142 kb on - strand at 2508.307 kb on + strand, within Pf6N2E2_2111 at 2508.308 kb on - strand, within Pf6N2E2_2111 at 2508.348 kb on + strand, within Pf6N2E2_2111 at 2508.348 kb on + strand at 2508.348 kb on + strand, within Pf6N2E2_2111 at 2508.349 kb on - strand, within Pf6N2E2_2111 at 2508.349 kb on - strand, within Pf6N2E2_2111 at 2508.349 kb on - strand, within Pf6N2E2_2111 at 2508.744 kb on + strand at 2508.745 kb on - strand at 2508.839 kb on - strand at 2508.839 kb on - strand at 2508.902 kb on + strand at 2508.903 kb on - strand at 2508.903 kb on - strand at 2508.905 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 2,506,046 + -0.5 2,506,046 + +1.5 2,506,046 + -0.3 2,506,046 + -1.6 2,506,046 + +0.7 2,506,046 + -0.3 2,506,046 + -0.1 2,506,047 - -0.5 2,506,047 - +0.6 2,506,047 - +0.8 2,506,047 - +0.3 2,506,047 - +0.7 2,506,047 - -1.1 2,506,047 - -0.2 2,506,047 - +1.4 2,506,047 - +0.9 2,506,047 - +0.1 2,506,047 - -0.4 2,506,047 - -0.7 2,506,047 - +0.7 2,506,047 - +1.9 2,506,047 - +0.4 2,506,216 + +2.0 2,506,216 + -0.3 2,506,216 + +1.2 2,506,216 + +0.7 2,506,216 + +0.9 2,506,216 + +0.7 2,506,216 + -0.3 2,506,217 - +0.8 2,506,217 - +0.5 2,506,217 - -0.7 2,506,217 - -2.6 2,506,217 - -0.4 2,506,217 - -0.3 2,506,330 + Pf6N2E2_2109 0.19 -0.1 2,506,330 + Pf6N2E2_2109 0.19 -0.1 2,506,330 + Pf6N2E2_2109 0.19 -0.3 2,506,331 - Pf6N2E2_2109 0.19 +1.0 2,506,331 - Pf6N2E2_2109 0.19 +0.4 2,506,331 - Pf6N2E2_2109 0.19 -0.3 2,506,855 + Pf6N2E2_2109 0.81 -0.3 2,506,856 - Pf6N2E2_2109 0.82 -0.6 2,506,856 - Pf6N2E2_2109 0.82 -0.2 2,507,008 + +0.3 2,507,008 + -0.1 2,507,009 - -1.6 2,507,009 - +0.2 2,507,461 + Pf6N2E2_2110 0.47 +0.2 2,507,461 + Pf6N2E2_2110 0.47 -1.2 2,507,462 - Pf6N2E2_2110 0.47 -0.2 2,507,462 - Pf6N2E2_2110 0.47 -1.5 2,507,462 - Pf6N2E2_2110 0.47 -1.5 2,507,792 + Pf6N2E2_2110 0.83 -0.3 2,507,792 + Pf6N2E2_2110 0.83 -0.5 2,507,793 - Pf6N2E2_2110 0.83 -0.4 2,507,972 + +0.1 2,507,973 - -0.1 2,508,097 + +0.8 2,508,097 + -0.1 2,508,098 - -0.6 2,508,098 - -1.2 2,508,142 - -1.1 2,508,307 + Pf6N2E2_2111 0.28 +0.4 2,508,308 - Pf6N2E2_2111 0.28 -1.7 2,508,348 + Pf6N2E2_2111 0.34 -0.1 2,508,348 + +2.7 2,508,348 + Pf6N2E2_2111 0.34 +0.1 2,508,349 - Pf6N2E2_2111 0.34 -1.3 2,508,349 - Pf6N2E2_2111 0.34 +0.1 2,508,349 - Pf6N2E2_2111 0.34 +0.4 2,508,744 + +0.6 2,508,745 - +0.6 2,508,839 - +0.4 2,508,839 - -0.8 2,508,902 + -1.4 2,508,903 - -1.0 2,508,903 - -0.2 2,508,905 + -0.1
Or see this region's nucleotide sequence