Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1919 and Pf6N2E2_1920 are separated by 87 nucleotides Pf6N2E2_1920 and Pf6N2E2_1921 are separated by 22 nucleotides Pf6N2E2_1921 and Pf6N2E2_1922 overlap by 4 nucleotides
Pf6N2E2_1919 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,273,106 to 2,274,578
_1919
Pf6N2E2_1920 - Rubredoxin, at 2,274,666 to 2,274,872
_1920
Pf6N2E2_1921 - hypothetical protein, at 2,274,895 to 2,275,032
_1921
Pf6N2E2_1922 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,275,029 to 2,277,140
_1922
Position (kb)
2274
2275 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2273.995 kb on - strand, within Pf6N2E2_1919 at 2274.259 kb on - strand, within Pf6N2E2_1919 at 2274.259 kb on - strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.342 kb on - strand, within Pf6N2E2_1919 at 2274.344 kb on + strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.557 kb on + strand at 2274.558 kb on - strand at 2274.558 kb on - strand at 2274.558 kb on - strand at 2274.664 kb on - strand at 2274.664 kb on - strand at 2274.760 kb on - strand, within Pf6N2E2_1920 at 2274.760 kb on - strand, within Pf6N2E2_1920 at 2274.777 kb on - strand, within Pf6N2E2_1920 at 2274.777 kb on - strand, within Pf6N2E2_1920 at 2274.896 kb on + strand at 2274.935 kb on + strand, within Pf6N2E2_1921 at 2275.033 kb on - strand at 2275.033 kb on - strand at 2275.033 kb on - strand at 2275.094 kb on + strand at 2275.115 kb on - strand at 2275.178 kb on - strand at 2275.216 kb on + strand at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.299 kb on + strand, within Pf6N2E2_1922 at 2275.299 kb on + strand, within Pf6N2E2_1922 at 2275.335 kb on - strand, within Pf6N2E2_1922 at 2275.430 kb on - strand, within Pf6N2E2_1922 at 2275.501 kb on + strand, within Pf6N2E2_1922 at 2275.502 kb on - strand, within Pf6N2E2_1922 at 2275.541 kb on - strand, within Pf6N2E2_1922 at 2275.650 kb on + strand, within Pf6N2E2_1922 at 2275.651 kb on - strand, within Pf6N2E2_1922
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 2,273,995 - Pf6N2E2_1919 0.60 +0.6 2,274,259 - Pf6N2E2_1919 0.78 -1.1 2,274,259 - Pf6N2E2_1919 0.78 -0.2 2,274,341 + Pf6N2E2_1919 0.84 +1.1 2,274,341 + Pf6N2E2_1919 0.84 -1.7 2,274,341 + Pf6N2E2_1919 0.84 -0.8 2,274,342 - Pf6N2E2_1919 0.84 +0.4 2,274,344 + Pf6N2E2_1919 0.84 -1.2 2,274,345 - Pf6N2E2_1919 0.84 +1.5 2,274,345 - Pf6N2E2_1919 0.84 +0.4 2,274,345 - Pf6N2E2_1919 0.84 -0.2 2,274,557 + -0.1 2,274,558 - -0.1 2,274,558 - +0.3 2,274,558 - -0.1 2,274,664 - -0.6 2,274,664 - -0.6 2,274,760 - Pf6N2E2_1920 0.45 -0.3 2,274,760 - Pf6N2E2_1920 0.45 -0.5 2,274,777 - Pf6N2E2_1920 0.54 -1.0 2,274,777 - Pf6N2E2_1920 0.54 -1.3 2,274,896 + +0.4 2,274,935 + Pf6N2E2_1921 0.29 +2.5 2,275,033 - -0.6 2,275,033 - +1.0 2,275,033 - -0.8 2,275,094 + -0.3 2,275,115 - -1.4 2,275,178 - -0.9 2,275,216 + +0.8 2,275,257 - Pf6N2E2_1922 0.11 -0.1 2,275,257 - Pf6N2E2_1922 0.11 +0.8 2,275,257 - Pf6N2E2_1922 0.11 -0.8 2,275,299 + Pf6N2E2_1922 0.13 +1.0 2,275,299 + Pf6N2E2_1922 0.13 -2.5 2,275,335 - Pf6N2E2_1922 0.14 -0.0 2,275,430 - Pf6N2E2_1922 0.19 -0.3 2,275,501 + Pf6N2E2_1922 0.22 +0.2 2,275,502 - Pf6N2E2_1922 0.22 +0.6 2,275,541 - Pf6N2E2_1922 0.24 -0.0 2,275,650 + Pf6N2E2_1922 0.29 -0.1 2,275,651 - Pf6N2E2_1922 0.29 +1.0
Or see this region's nucleotide sequence