Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_159 and Pf6N2E2_160 are separated by 248 nucleotides Pf6N2E2_160 and Pf6N2E2_161 are separated by 133 nucleotides Pf6N2E2_161 and Pf6N2E2_162 are separated by 58 nucleotides
Pf6N2E2_159 - DNA damage resistance protein (from data) , at 213,204 to 213,452
_159
Pf6N2E2_160 - hypothetical protein, at 213,701 to 214,147
_160
Pf6N2E2_161 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 214,281 to 215,237
_161
Pf6N2E2_162 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 215,296 to 216,849
_162
Position (kb)
214
215
216 Strain fitness (log2 ratio)
-2
-1
0
1 at 213.411 kb on - strand, within Pf6N2E2_159 at 213.411 kb on - strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.412 kb on + strand, within Pf6N2E2_159 at 213.413 kb on - strand, within Pf6N2E2_159 at 213.413 kb on - strand, within Pf6N2E2_159 at 213.420 kb on + strand, within Pf6N2E2_159 at 213.421 kb on - strand, within Pf6N2E2_159 at 213.428 kb on + strand at 213.580 kb on + strand at 213.592 kb on + strand at 213.592 kb on + strand at 213.593 kb on - strand at 213.995 kb on + strand, within Pf6N2E2_160 at 213.996 kb on - strand, within Pf6N2E2_160 at 214.069 kb on - strand, within Pf6N2E2_160 at 214.135 kb on + strand at 214.135 kb on + strand at 214.135 kb on + strand at 214.135 kb on + strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.136 kb on - strand at 214.197 kb on + strand at 214.198 kb on - strand at 214.202 kb on + strand at 214.202 kb on + strand at 214.202 kb on + strand at 214.203 kb on - strand at 214.203 kb on - strand at 214.257 kb on + strand at 214.257 kb on + strand at 214.257 kb on + strand at 214.258 kb on - strand at 214.258 kb on - strand at 214.264 kb on - strand at 214.264 kb on - strand at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.456 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161 at 215.235 kb on + strand at 215.235 kb on + strand at 215.235 kb on + strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.240 kb on - strand at 215.240 kb on - strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.296 kb on - strand at 215.350 kb on + strand at 215.350 kb on + strand at 215.351 kb on - strand at 215.351 kb on - strand at 215.351 kb on - strand at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 216.087 kb on + strand, within Pf6N2E2_162 at 216.156 kb on + strand, within Pf6N2E2_162
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 213,411 - Pf6N2E2_159 0.83 +1.2 213,411 - Pf6N2E2_159 0.83 +0.4 213,412 + Pf6N2E2_159 0.84 +0.3 213,412 + Pf6N2E2_159 0.84 -2.2 213,412 + Pf6N2E2_159 0.84 -1.1 213,413 - Pf6N2E2_159 0.84 -0.3 213,413 - Pf6N2E2_159 0.84 -0.4 213,420 + Pf6N2E2_159 0.87 +0.4 213,421 - Pf6N2E2_159 0.87 -0.4 213,428 + +0.0 213,580 + +0.4 213,592 + +0.2 213,592 + -1.3 213,593 - -0.1 213,995 + Pf6N2E2_160 0.66 +1.2 213,996 - Pf6N2E2_160 0.66 +0.0 214,069 - Pf6N2E2_160 0.82 -0.1 214,135 + +0.5 214,135 + -0.7 214,135 + -0.1 214,135 + -2.5 214,136 - -0.4 214,136 - +0.3 214,136 - +0.1 214,136 - -0.6 214,136 - +0.1 214,136 - +1.3 214,136 - -1.3 214,197 + -0.7 214,198 - +0.5 214,202 + -1.7 214,202 + -1.4 214,202 + +0.4 214,203 - +0.1 214,203 - +0.4 214,257 + -0.0 214,257 + -0.2 214,257 + -0.4 214,258 - -0.0 214,258 - +0.3 214,264 - -0.5 214,264 - +0.6 214,455 + Pf6N2E2_161 0.18 -1.1 214,455 + Pf6N2E2_161 0.18 +0.8 214,455 + Pf6N2E2_161 0.18 +0.2 214,456 - Pf6N2E2_161 0.18 -1.6 214,753 - Pf6N2E2_161 0.49 -0.8 214,753 - Pf6N2E2_161 0.49 -0.4 215,235 + +0.1 215,235 + +0.8 215,235 + +0.0 215,236 - +0.0 215,236 - +0.5 215,236 - -0.3 215,236 - +0.2 215,240 - -0.1 215,240 - +0.5 215,295 + -1.0 215,295 + +0.6 215,295 + +0.9 215,295 + -0.7 215,295 + -0.1 215,295 + +0.7 215,295 + -0.9 215,295 + -0.6 215,295 + +0.2 215,296 - +0.4 215,350 + -0.8 215,350 + +0.6 215,351 - -1.1 215,351 - +0.2 215,351 - +0.5 215,893 + Pf6N2E2_162 0.38 -1.9 215,893 + Pf6N2E2_162 0.38 +0.7 215,893 + Pf6N2E2_162 0.38 +0.1 215,893 + Pf6N2E2_162 0.38 -0.6 215,893 + Pf6N2E2_162 0.38 +0.1 215,893 + Pf6N2E2_162 0.38 -0.2 215,894 - Pf6N2E2_162 0.38 -0.7 215,894 - Pf6N2E2_162 0.38 +0.2 215,894 - Pf6N2E2_162 0.38 -0.6 215,894 - Pf6N2E2_162 0.38 -0.2 215,894 - Pf6N2E2_162 0.38 -0.3 215,989 + Pf6N2E2_162 0.45 +0.4 215,989 + Pf6N2E2_162 0.45 -0.5 215,989 + Pf6N2E2_162 0.45 -0.8 215,989 + Pf6N2E2_162 0.45 -0.1 215,990 - Pf6N2E2_162 0.45 +0.4 215,990 - Pf6N2E2_162 0.45 -0.5 215,990 - Pf6N2E2_162 0.45 -0.4 216,087 + Pf6N2E2_162 0.51 -0.1 216,156 + Pf6N2E2_162 0.55 +0.0
Or see this region's nucleotide sequence