Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1521 and Pf6N2E2_1522 are separated by 170 nucleotides Pf6N2E2_1522 and Pf6N2E2_1523 overlap by 11 nucleotides
Pf6N2E2_1521 - Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) @ Fructose-6-phosphate phosphoketolase (EC 4.1.2.22), at 1,777,559 to 1,779,931
_1521
Pf6N2E2_1522 - Alcohol dehydrogenase (EC 1.1.1.1), at 1,780,102 to 1,781,085
_1522
Pf6N2E2_1523 - Universal stress protein family 5, at 1,781,075 to 1,781,968
_1523
Position (kb)
1780
1781
1782 Strain fitness (log2 ratio)
-1
0
1
2 at 1779.115 kb on + strand, within Pf6N2E2_1521 at 1779.116 kb on - strand, within Pf6N2E2_1521 at 1779.234 kb on + strand, within Pf6N2E2_1521 at 1779.234 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.255 kb on + strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.256 kb on - strand, within Pf6N2E2_1521 at 1779.376 kb on + strand, within Pf6N2E2_1521 at 1779.474 kb on + strand, within Pf6N2E2_1521 at 1779.551 kb on + strand, within Pf6N2E2_1521 at 1779.621 kb on + strand, within Pf6N2E2_1521 at 1779.637 kb on + strand, within Pf6N2E2_1521 at 1779.648 kb on + strand, within Pf6N2E2_1521 at 1779.649 kb on - strand, within Pf6N2E2_1521 at 1779.649 kb on - strand, within Pf6N2E2_1521 at 1779.649 kb on - strand, within Pf6N2E2_1521 at 1779.846 kb on + strand at 1779.846 kb on + strand at 1779.846 kb on + strand at 1779.847 kb on - strand at 1779.895 kb on + strand at 1779.896 kb on - strand at 1780.001 kb on + strand at 1780.002 kb on - strand at 1780.022 kb on - strand at 1780.029 kb on + strand at 1780.029 kb on + strand at 1780.030 kb on - strand at 1780.030 kb on - strand at 1780.465 kb on + strand, within Pf6N2E2_1522 at 1780.604 kb on - strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.666 kb on + strand, within Pf6N2E2_1522 at 1780.667 kb on - strand, within Pf6N2E2_1522 at 1781.006 kb on - strand at 1781.072 kb on + strand at 1781.073 kb on - strand at 1781.073 kb on - strand at 1781.112 kb on + strand at 1781.112 kb on + strand at 1781.113 kb on - strand at 1781.290 kb on + strand, within Pf6N2E2_1523 at 1781.291 kb on - strand, within Pf6N2E2_1523 at 1781.397 kb on + strand, within Pf6N2E2_1523 at 1781.398 kb on - strand, within Pf6N2E2_1523 at 1781.626 kb on + strand, within Pf6N2E2_1523 at 1781.703 kb on - strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.822 kb on + strand, within Pf6N2E2_1523 at 1781.823 kb on - strand, within Pf6N2E2_1523 at 1781.823 kb on - strand, within Pf6N2E2_1523 at 1781.946 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 1,779,115 + Pf6N2E2_1521 0.66 +1.0 1,779,116 - Pf6N2E2_1521 0.66 +0.0 1,779,234 + Pf6N2E2_1521 0.71 +0.9 1,779,234 + Pf6N2E2_1521 0.71 +0.3 1,779,255 + Pf6N2E2_1521 0.71 +1.8 1,779,255 + Pf6N2E2_1521 0.71 +0.8 1,779,255 + Pf6N2E2_1521 0.71 -0.2 1,779,255 + Pf6N2E2_1521 0.71 -0.3 1,779,255 + Pf6N2E2_1521 0.71 +0.1 1,779,256 - Pf6N2E2_1521 0.72 +0.3 1,779,256 - Pf6N2E2_1521 0.72 -0.3 1,779,256 - Pf6N2E2_1521 0.72 -0.9 1,779,256 - Pf6N2E2_1521 0.72 -0.1 1,779,256 - Pf6N2E2_1521 0.72 +0.7 1,779,376 + Pf6N2E2_1521 0.77 -0.7 1,779,474 + Pf6N2E2_1521 0.81 -0.1 1,779,551 + Pf6N2E2_1521 0.84 -0.7 1,779,621 + Pf6N2E2_1521 0.87 +0.9 1,779,637 + Pf6N2E2_1521 0.88 -0.0 1,779,648 + Pf6N2E2_1521 0.88 -0.4 1,779,649 - Pf6N2E2_1521 0.88 +1.3 1,779,649 - Pf6N2E2_1521 0.88 +0.4 1,779,649 - Pf6N2E2_1521 0.88 -0.8 1,779,846 + +1.6 1,779,846 + -0.1 1,779,846 + +1.7 1,779,847 - -0.2 1,779,895 + +0.3 1,779,896 - -0.2 1,780,001 + -0.5 1,780,002 - -0.8 1,780,022 - -1.4 1,780,029 + +0.3 1,780,029 + -1.2 1,780,030 - +0.3 1,780,030 - -1.7 1,780,465 + Pf6N2E2_1522 0.37 -0.3 1,780,604 - Pf6N2E2_1522 0.51 -0.9 1,780,666 + Pf6N2E2_1522 0.57 +0.1 1,780,666 + Pf6N2E2_1522 0.57 -0.6 1,780,666 + Pf6N2E2_1522 0.57 +0.4 1,780,666 + Pf6N2E2_1522 0.57 -1.2 1,780,667 - Pf6N2E2_1522 0.57 -1.2 1,781,006 - -0.1 1,781,072 + +0.3 1,781,073 - +0.2 1,781,073 - -1.5 1,781,112 + +0.0 1,781,112 + -0.1 1,781,113 - -0.9 1,781,290 + Pf6N2E2_1523 0.24 -1.6 1,781,291 - Pf6N2E2_1523 0.24 -0.3 1,781,397 + Pf6N2E2_1523 0.36 +0.5 1,781,398 - Pf6N2E2_1523 0.36 -0.2 1,781,626 + Pf6N2E2_1523 0.62 -0.0 1,781,703 - Pf6N2E2_1523 0.70 +0.2 1,781,822 + Pf6N2E2_1523 0.84 -1.4 1,781,822 + Pf6N2E2_1523 0.84 -1.6 1,781,822 + Pf6N2E2_1523 0.84 -0.4 1,781,823 - Pf6N2E2_1523 0.84 +0.8 1,781,823 - Pf6N2E2_1523 0.84 -0.6 1,781,946 - -0.8
Or see this region's nucleotide sequence