Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1175 and Pf6N2E2_1176 are separated by 73 nucleotides Pf6N2E2_1176 and Pf6N2E2_1177 are separated by 12 nucleotides
Pf6N2E2_1175 - VgrG protein, at 1,375,292 to 1,377,517
_1175
Pf6N2E2_1176 - Mll2356 protein, at 1,377,591 to 1,377,986
_1176
Pf6N2E2_1177 - putative exported protein, at 1,377,999 to 1,379,141
_1177
Position (kb)
1377
1378 Strain fitness (log2 ratio)
-1
0
1
2 at 1377.197 kb on - strand, within Pf6N2E2_1175 at 1377.395 kb on - strand at 1377.395 kb on - strand at 1377.515 kb on + strand at 1377.515 kb on + strand at 1377.534 kb on - strand at 1377.546 kb on + strand at 1377.683 kb on + strand, within Pf6N2E2_1176 at 1377.907 kb on + strand, within Pf6N2E2_1176 at 1377.907 kb on + strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.973 kb on + strand at 1378.037 kb on + strand at 1378.037 kb on + strand at 1378.038 kb on - strand at 1378.096 kb on - strand at 1378.169 kb on - strand, within Pf6N2E2_1177 at 1378.204 kb on - strand, within Pf6N2E2_1177 at 1378.356 kb on + strand, within Pf6N2E2_1177 at 1378.356 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.649 kb on + strand, within Pf6N2E2_1177 at 1378.650 kb on - strand, within Pf6N2E2_1177 at 1378.668 kb on + strand, within Pf6N2E2_1177 at 1378.668 kb on + strand, within Pf6N2E2_1177 at 1378.669 kb on - strand, within Pf6N2E2_1177
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 1,377,197 - Pf6N2E2_1175 0.86 +1.0 1,377,395 - -0.2 1,377,395 - +0.0 1,377,515 + +0.9 1,377,515 + -0.1 1,377,534 - +2.0 1,377,546 + +0.2 1,377,683 + Pf6N2E2_1176 0.23 -0.2 1,377,907 + Pf6N2E2_1176 0.80 -1.1 1,377,907 + Pf6N2E2_1176 0.80 +1.3 1,377,908 - Pf6N2E2_1176 0.80 -1.6 1,377,908 - Pf6N2E2_1176 0.80 +0.3 1,377,908 - Pf6N2E2_1176 0.80 -1.0 1,377,908 - Pf6N2E2_1176 0.80 -0.2 1,377,973 + +0.3 1,378,037 + -0.2 1,378,037 + -0.1 1,378,038 - +0.3 1,378,096 - +0.6 1,378,169 - Pf6N2E2_1177 0.15 -0.9 1,378,204 - Pf6N2E2_1177 0.18 +1.5 1,378,356 + Pf6N2E2_1177 0.31 -0.7 1,378,356 + Pf6N2E2_1177 0.31 -1.0 1,378,602 + Pf6N2E2_1177 0.53 +0.0 1,378,602 + Pf6N2E2_1177 0.53 +0.2 1,378,602 + Pf6N2E2_1177 0.53 +0.6 1,378,602 + Pf6N2E2_1177 0.53 +0.3 1,378,603 - Pf6N2E2_1177 0.53 -1.4 1,378,603 - Pf6N2E2_1177 0.53 -0.2 1,378,603 - Pf6N2E2_1177 0.53 -1.7 1,378,649 + Pf6N2E2_1177 0.57 +0.8 1,378,650 - Pf6N2E2_1177 0.57 +0.7 1,378,668 + Pf6N2E2_1177 0.59 +0.3 1,378,668 + Pf6N2E2_1177 0.59 -0.4 1,378,669 - Pf6N2E2_1177 0.59 -0.9
Or see this region's nucleotide sequence