Experiment: Varel_Bryant medium with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT_3258 and BT3259 are separated by 107 nucleotides BT3259 and BT3260 are separated by 332 nucleotides BT3260 and BT3261 are separated by 88 nucleotides
BT_3258: BT_3258 - two-component system response regulator (NCBI), at 4,160,463 to 4,161,242
_3258
BT3259: BT3259 - hypothetical protein (NCBI ptt file), at 4,161,350 to 4,161,745
BT3259
BT3260: BT3260 - DNA polymerase I (NCBI ptt file), at 4,162,078 to 4,164,927
BT3260
BT3261: BT3261 - octaprenyl-diphosphate synthase (NCBI ptt file), at 4,165,016 to 4,165,990
BT3261
Position (kb)
4162
4163
4164
4165 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4161.086 kb on + strand, within BT_3258 at 4161.176 kb on + strand at 4161.254 kb on + strand at 4161.254 kb on + strand at 4161.259 kb on + strand at 4161.259 kb on + strand at 4161.308 kb on + strand at 4161.309 kb on - strand at 4161.309 kb on - strand at 4161.329 kb on + strand at 4161.329 kb on + strand at 4161.352 kb on - strand at 4161.359 kb on - strand at 4161.361 kb on + strand at 4161.400 kb on - strand, within BT3259 at 4161.425 kb on - strand, within BT3259 at 4161.456 kb on + strand, within BT3259 at 4161.460 kb on + strand, within BT3259 at 4161.461 kb on - strand, within BT3259 at 4161.461 kb on - strand, within BT3259 at 4161.550 kb on - strand, within BT3259 at 4161.553 kb on + strand, within BT3259 at 4161.555 kb on + strand, within BT3259 at 4161.561 kb on + strand, within BT3259 at 4161.568 kb on + strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.584 kb on - strand, within BT3259 at 4161.614 kb on + strand, within BT3259 at 4161.643 kb on + strand, within BT3259 at 4161.643 kb on + strand, within BT3259 at 4161.663 kb on + strand, within BT3259 at 4161.664 kb on - strand, within BT3259 at 4161.704 kb on + strand, within BT3259 at 4161.711 kb on - strand at 4161.759 kb on + strand at 4161.795 kb on + strand at 4161.795 kb on + strand at 4161.796 kb on - strand at 4161.831 kb on - strand at 4161.847 kb on - strand at 4162.044 kb on + strand at 4162.067 kb on + strand at 4162.075 kb on - strand at 4162.080 kb on - strand at 4162.178 kb on - strand at 4162.178 kb on - strand at 4162.226 kb on - strand at 4162.250 kb on - strand at 4162.997 kb on - strand, within BT3260 at 4163.251 kb on - strand, within BT3260 at 4163.254 kb on - strand, within BT3260 at 4163.381 kb on + strand, within BT3260 at 4163.951 kb on - strand, within BT3260 at 4163.951 kb on - strand, within BT3260 at 4164.971 kb on + strand at 4164.983 kb on + strand at 4164.983 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Dimetridazole 0.025 mM remove 4,161,086 + BT_3258 0.80 -2.6 4,161,176 + -0.9 4,161,254 + -0.5 4,161,254 + -2.0 4,161,259 + -0.3 4,161,259 + -1.8 4,161,308 + -0.7 4,161,309 - -1.0 4,161,309 - +1.0 4,161,329 + -1.8 4,161,329 + -2.0 4,161,352 - -2.0 4,161,359 - -0.8 4,161,361 + -1.0 4,161,400 - BT3259 0.13 -0.1 4,161,425 - BT3259 0.19 -0.5 4,161,456 + BT3259 0.27 -1.6 4,161,460 + BT3259 0.28 -0.2 4,161,461 - BT3259 0.28 -1.2 4,161,461 - BT3259 0.28 -0.3 4,161,550 - BT3259 0.51 -1.5 4,161,553 + BT3259 0.51 -0.3 4,161,555 + BT3259 0.52 -0.5 4,161,561 + BT3259 0.53 -0.7 4,161,568 + BT3259 0.55 -0.5 4,161,584 - BT3259 0.59 -1.4 4,161,584 - BT3259 0.59 -2.0 4,161,584 - BT3259 0.59 -0.7 4,161,614 + BT3259 0.67 +0.3 4,161,643 + BT3259 0.74 -0.9 4,161,643 + BT3259 0.74 -1.2 4,161,663 + BT3259 0.79 +3.4 4,161,664 - BT3259 0.79 -1.3 4,161,704 + BT3259 0.89 -1.5 4,161,711 - -0.5 4,161,759 + -1.3 4,161,795 + -1.5 4,161,795 + +0.0 4,161,796 - -0.5 4,161,831 - -1.1 4,161,847 - +1.3 4,162,044 + -1.0 4,162,067 + +0.0 4,162,075 - +0.0 4,162,080 - -2.4 4,162,178 - -2.8 4,162,178 - -4.1 4,162,226 - -3.3 4,162,250 - -1.8 4,162,997 - BT3260 0.32 -1.5 4,163,251 - BT3260 0.41 -2.3 4,163,254 - BT3260 0.41 -3.3 4,163,381 + BT3260 0.46 -3.3 4,163,951 - BT3260 0.66 -2.8 4,163,951 - BT3260 0.66 -3.0 4,164,971 + -1.9 4,164,983 + +1.0 4,164,983 + -1.2
Or see this region's nucleotide sequence