Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS20850

Experiment: Plant=Bonny_Best_Tomato; Treatment=2dpi_Stem_Attached; Sample=Stem; Collection=Centrifuged_to_remove_stem_sap_homogenized_filtered_pelleted

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS20845 and RS_RS20850 overlap by 14 nucleotidesRS_RS20850 and RS_RS20855 overlap by 4 nucleotidesRS_RS20855 and RS_RS20860 overlap by 17 nucleotides RS_RS20845: RS_RS20845 - PLP-dependent transferase, at 982,886 to 984,427 _RS20845 RS_RS20850: RS_RS20850 - aminoacyl-tRNA synthet, at 984,414 to 986,366 _RS20850 RS_RS20855: RS_RS20855 - acyl carrier protein, at 986,363 to 986,632 _RS20855 RS_RS20860: RS_RS20860 - omega-3 fatty acid desaturase, at 986,616 to 987,593 _RS20860 Position (kb) 984 985 986 987Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 983.504 kb on + strand, within RS_RS20845at 983.504 kb on + strand, within RS_RS20845at 983.504 kb on + strand, within RS_RS20845at 983.504 kb on + strand, within RS_RS20845at 983.505 kb on - strand, within RS_RS20845at 983.505 kb on - strand, within RS_RS20845at 983.511 kb on - strand, within RS_RS20845at 983.511 kb on - strand, within RS_RS20845at 983.573 kb on + strand, within RS_RS20845at 983.573 kb on + strand, within RS_RS20845at 983.574 kb on - strand, within RS_RS20845at 983.590 kb on - strand, within RS_RS20845at 983.801 kb on + strand, within RS_RS20845at 983.802 kb on - strand, within RS_RS20845at 983.802 kb on - strand, within RS_RS20845at 983.802 kb on - strand, within RS_RS20845at 983.802 kb on - strand, within RS_RS20845at 983.804 kb on + strand, within RS_RS20845at 983.804 kb on + strand, within RS_RS20845at 983.805 kb on - strand, within RS_RS20845at 983.805 kb on - strand, within RS_RS20845at 983.822 kb on + strand, within RS_RS20845at 983.859 kb on - strand, within RS_RS20845at 984.023 kb on + strand, within RS_RS20845at 984.023 kb on + strand, within RS_RS20845at 984.023 kb on + strand, within RS_RS20845at 984.024 kb on - strand, within RS_RS20845at 984.024 kb on - strand, within RS_RS20845at 984.069 kb on - strand, within RS_RS20845at 984.164 kb on + strand, within RS_RS20845at 984.305 kb on + strandat 984.306 kb on - strandat 984.306 kb on - strandat 984.392 kb on + strandat 984.393 kb on - strandat 984.393 kb on - strandat 984.393 kb on - strandat 984.568 kb on - strandat 984.568 kb on - strandat 984.577 kb on - strandat 984.651 kb on + strand, within RS_RS20850at 984.651 kb on + strand, within RS_RS20850at 984.652 kb on - strand, within RS_RS20850at 984.843 kb on + strand, within RS_RS20850at 984.843 kb on + strand, within RS_RS20850at 984.844 kb on - strand, within RS_RS20850at 984.844 kb on - strand, within RS_RS20850at 984.844 kb on - strand, within RS_RS20850at 984.849 kb on + strand, within RS_RS20850at 984.849 kb on + strand, within RS_RS20850at 984.849 kb on + strand, within RS_RS20850at 984.850 kb on - strand, within RS_RS20850at 984.850 kb on - strand, within RS_RS20850at 984.991 kb on - strand, within RS_RS20850at 985.215 kb on + strand, within RS_RS20850at 985.215 kb on + strand, within RS_RS20850at 985.215 kb on + strand, within RS_RS20850at 985.215 kb on + strand, within RS_RS20850at 985.216 kb on - strand, within RS_RS20850at 985.216 kb on - strand, within RS_RS20850at 985.216 kb on - strand, within RS_RS20850at 985.216 kb on - strand, within RS_RS20850at 985.216 kb on - strand, within RS_RS20850at 985.407 kb on + strand, within RS_RS20850at 985.407 kb on + strand, within RS_RS20850at 985.407 kb on + strand, within RS_RS20850at 985.823 kb on - strand, within RS_RS20850at 985.824 kb on + strand, within RS_RS20850at 985.824 kb on + strand, within RS_RS20850at 985.824 kb on + strand, within RS_RS20850at 985.824 kb on + strand, within RS_RS20850at 985.825 kb on - strand, within RS_RS20850at 985.825 kb on - strand, within RS_RS20850at 985.825 kb on - strand, within RS_RS20850at 985.825 kb on - strand, within RS_RS20850at 985.825 kb on - strand, within RS_RS20850at 985.830 kb on + strand, within RS_RS20850at 985.831 kb on - strand, within RS_RS20850at 985.831 kb on - strand, within RS_RS20850at 986.016 kb on + strand, within RS_RS20850at 986.016 kb on + strand, within RS_RS20850at 986.017 kb on - strand, within RS_RS20850at 986.754 kb on + strand, within RS_RS20860at 986.755 kb on - strand, within RS_RS20860at 987.051 kb on + strand, within RS_RS20860at 987.052 kb on - strand, within RS_RS20860at 987.099 kb on + strand, within RS_RS20860at 987.309 kb on + strand, within RS_RS20860at 987.333 kb on + strand, within RS_RS20860at 987.333 kb on + strand, within RS_RS20860at 987.333 kb on + strand, within RS_RS20860at 987.334 kb on - strand, within RS_RS20860

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=Bonny_Best_Tomato; Treatment=2dpi_Stem_Attached; Sample=Stem; Collection=Centrifuged_to_remove_stem_sap_homogenized_filtered_pelleted
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983,504 + RS_RS20845 0.40 -0.4
983,504 + RS_RS20845 0.40 -0.7
983,504 + RS_RS20845 0.40 +0.5
983,504 + RS_RS20845 0.40 +1.0
983,505 - RS_RS20845 0.40 -3.0
983,505 - RS_RS20845 0.40 +0.8
983,511 - RS_RS20845 0.41 -0.6
983,511 - RS_RS20845 0.41 -0.6
983,573 + RS_RS20845 0.45 +0.5
983,573 + RS_RS20845 0.45 +0.6
983,574 - RS_RS20845 0.45 +0.2
983,590 - RS_RS20845 0.46 -0.2
983,801 + RS_RS20845 0.59 -1.8
983,802 - RS_RS20845 0.59 -0.3
983,802 - RS_RS20845 0.59 +1.0
983,802 - RS_RS20845 0.59 +0.3
983,802 - RS_RS20845 0.59 +0.7
983,804 + RS_RS20845 0.60 -1.1
983,804 + RS_RS20845 0.60 +1.3
983,805 - RS_RS20845 0.60 +0.3
983,805 - RS_RS20845 0.60 -0.5
983,822 + RS_RS20845 0.61 -0.8
983,859 - RS_RS20845 0.63 +0.7
984,023 + RS_RS20845 0.74 -2.0
984,023 + RS_RS20845 0.74 -2.0
984,023 + RS_RS20845 0.74 -0.4
984,024 - RS_RS20845 0.74 -2.0
984,024 - RS_RS20845 0.74 -3.8
984,069 - RS_RS20845 0.77 -1.7
984,164 + RS_RS20845 0.83 -1.2
984,305 + -0.3
984,306 - +0.5
984,306 - -0.1
984,392 + -0.9
984,393 - -1.9
984,393 - +0.2
984,393 - +0.5
984,568 - -1.9
984,568 - -0.7
984,577 - -0.1
984,651 + RS_RS20850 0.12 -0.6
984,651 + RS_RS20850 0.12 -1.2
984,652 - RS_RS20850 0.12 +1.0
984,843 + RS_RS20850 0.22 +0.4
984,843 + RS_RS20850 0.22 -0.3
984,844 - RS_RS20850 0.22 -1.0
984,844 - RS_RS20850 0.22 -2.2
984,844 - RS_RS20850 0.22 -0.4
984,849 + RS_RS20850 0.22 -0.8
984,849 + RS_RS20850 0.22 -0.9
984,849 + RS_RS20850 0.22 -0.7
984,850 - RS_RS20850 0.22 +0.4
984,850 - RS_RS20850 0.22 +0.1
984,991 - RS_RS20850 0.30 +1.5
985,215 + RS_RS20850 0.41 +0.9
985,215 + RS_RS20850 0.41 -0.1
985,215 + RS_RS20850 0.41 +0.5
985,215 + RS_RS20850 0.41 -0.5
985,216 - RS_RS20850 0.41 +0.0
985,216 - RS_RS20850 0.41 -0.9
985,216 - RS_RS20850 0.41 +0.6
985,216 - RS_RS20850 0.41 +0.6
985,216 - RS_RS20850 0.41 +0.8
985,407 + RS_RS20850 0.51 -1.5
985,407 + RS_RS20850 0.51 -1.8
985,407 + RS_RS20850 0.51 +0.8
985,823 - RS_RS20850 0.72 -1.2
985,824 + RS_RS20850 0.72 +0.2
985,824 + RS_RS20850 0.72 +0.3
985,824 + RS_RS20850 0.72 -0.2
985,824 + RS_RS20850 0.72 -1.9
985,825 - RS_RS20850 0.72 +0.1
985,825 - RS_RS20850 0.72 -0.2
985,825 - RS_RS20850 0.72 -0.7
985,825 - RS_RS20850 0.72 -1.1
985,825 - RS_RS20850 0.72 -2.3
985,830 + RS_RS20850 0.73 +1.5
985,831 - RS_RS20850 0.73 +0.2
985,831 - RS_RS20850 0.73 -0.3
986,016 + RS_RS20850 0.82 +0.6
986,016 + RS_RS20850 0.82 -1.6
986,017 - RS_RS20850 0.82 +0.1
986,754 + RS_RS20860 0.14 +0.7
986,755 - RS_RS20860 0.14 -0.7
987,051 + RS_RS20860 0.44 -1.3
987,052 - RS_RS20860 0.45 -0.1
987,099 + RS_RS20860 0.49 +0.2
987,309 + RS_RS20860 0.71 +0.1
987,333 + RS_RS20860 0.73 -0.8
987,333 + RS_RS20860 0.73 +1.2
987,333 + RS_RS20860 0.73 -1.1
987,334 - RS_RS20860 0.73 +0.0

Or see this region's nucleotide sequence