Strain Fitness in Ralstonia solanacearum GMI1000 around RS_RS10060

Experiment: Plant=Bonny_Best_Tomato; Treatment=2dpi_Stem_Attached; Sample=Stem; Collection=Centrifuged_to_remove_stem_sap_homogenized_filtered_pelleted

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRS_RS10050 and RS_RS10055 are separated by 247 nucleotidesRS_RS10055 and RS_RS10060 are separated by 24 nucleotidesRS_RS10060 and RS_RS10065 are separated by 63 nucleotides RS_RS10050: RS_RS10050 - methylisocitrate lyase, at 2,176,893 to 2,177,789 _RS10050 RS_RS10055: RS_RS10055 - hypothetical protein, at 2,178,037 to 2,178,594 _RS10055 RS_RS10060: RS_RS10060 - bifunctional 2-methylcitrate dehydratase/aconitate hydratase, at 2,178,619 to 2,180,085 _RS10060 RS_RS10065: RS_RS10065 - aconitate hydratase AcnA, at 2,180,149 to 2,182,854 _RS10065 Position (kb) 2178 2179 2180 2181Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2177.721 kb on + strandat 2177.722 kb on - strandat 2177.745 kb on + strandat 2177.745 kb on + strandat 2177.842 kb on - strandat 2177.842 kb on - strandat 2177.842 kb on - strandat 2177.920 kb on + strandat 2178.095 kb on - strand, within RS_RS10055at 2178.244 kb on + strand, within RS_RS10055at 2178.244 kb on + strand, within RS_RS10055at 2178.245 kb on - strand, within RS_RS10055at 2178.245 kb on - strand, within RS_RS10055at 2178.305 kb on - strand, within RS_RS10055at 2178.305 kb on - strand, within RS_RS10055at 2178.305 kb on - strand, within RS_RS10055at 2178.718 kb on + strandat 2179.250 kb on - strand, within RS_RS10060at 2179.250 kb on - strand, within RS_RS10060at 2179.391 kb on - strand, within RS_RS10060at 2179.444 kb on + strand, within RS_RS10060at 2179.445 kb on - strand, within RS_RS10060at 2179.445 kb on - strand, within RS_RS10060at 2179.445 kb on - strand, within RS_RS10060at 2179.666 kb on + strand, within RS_RS10060at 2179.667 kb on - strand, within RS_RS10060at 2179.667 kb on - strand, within RS_RS10060at 2179.715 kb on - strand, within RS_RS10060at 2179.903 kb on + strand, within RS_RS10060at 2180.062 kb on + strandat 2180.074 kb on + strandat 2180.075 kb on - strandat 2180.710 kb on + strand, within RS_RS10065at 2180.731 kb on - strand, within RS_RS10065

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Per-strain Table

Position Strand Gene LocusTag Fraction Plant=Bonny_Best_Tomato; Treatment=2dpi_Stem_Attached; Sample=Stem; Collection=Centrifuged_to_remove_stem_sap_homogenized_filtered_pelleted
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2,177,721 + +0.4
2,177,722 - +0.6
2,177,745 + +0.3
2,177,745 + -0.8
2,177,842 - +0.4
2,177,842 - -0.5
2,177,842 - -0.2
2,177,920 + +1.2
2,178,095 - RS_RS10055 0.10 +1.4
2,178,244 + RS_RS10055 0.37 -0.2
2,178,244 + RS_RS10055 0.37 -2.7
2,178,245 - RS_RS10055 0.37 +2.3
2,178,245 - RS_RS10055 0.37 +1.4
2,178,305 - RS_RS10055 0.48 +1.9
2,178,305 - RS_RS10055 0.48 +1.0
2,178,305 - RS_RS10055 0.48 -2.2
2,178,718 + -1.0
2,179,250 - RS_RS10060 0.43 -1.3
2,179,250 - RS_RS10060 0.43 +1.6
2,179,391 - RS_RS10060 0.53 +3.6
2,179,444 + RS_RS10060 0.56 +1.4
2,179,445 - RS_RS10060 0.56 +2.5
2,179,445 - RS_RS10060 0.56 +0.4
2,179,445 - RS_RS10060 0.56 +1.8
2,179,666 + RS_RS10060 0.71 -0.9
2,179,667 - RS_RS10060 0.71 +0.8
2,179,667 - RS_RS10060 0.71 +1.1
2,179,715 - RS_RS10060 0.75 +1.1
2,179,903 + RS_RS10060 0.88 +0.3
2,180,062 + -1.2
2,180,074 + +0.6
2,180,075 - +2.5
2,180,710 + RS_RS10065 0.21 -0.7
2,180,731 - RS_RS10065 0.22 -1.0

Or see this region's nucleotide sequence