Experiment: bismuth(III) chloride 0.000025 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1581 and Pf6N2E2_1582 are separated by 2 nucleotides Pf6N2E2_1582 and Pf6N2E2_1583 overlap by 23 nucleotides
Pf6N2E2_1581 - Type I secretion outer membrane protein, TolC precursor, at 1,850,003 to 1,851,376
_1581
Pf6N2E2_1582 - HlyD family secretion protein, at 1,851,379 to 1,852,695
_1582
Pf6N2E2_1583 - ABC-type protease/lipase transport system, ATPase and permease components, at 1,852,673 to 1,854,457
_1583
Position (kb)
1851
1852
1853 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1850.415 kb on + strand, within Pf6N2E2_1581 at 1850.415 kb on + strand, within Pf6N2E2_1581 at 1850.435 kb on + strand, within Pf6N2E2_1581 at 1850.435 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.448 kb on + strand, within Pf6N2E2_1581 at 1850.449 kb on - strand, within Pf6N2E2_1581 at 1850.449 kb on - strand, within Pf6N2E2_1581 at 1850.449 kb on - strand, within Pf6N2E2_1581 at 1850.481 kb on + strand, within Pf6N2E2_1581 at 1850.835 kb on + strand, within Pf6N2E2_1581 at 1850.835 kb on + strand, within Pf6N2E2_1581 at 1850.928 kb on + strand, within Pf6N2E2_1581 at 1850.928 kb on + strand, within Pf6N2E2_1581 at 1850.928 kb on + strand, within Pf6N2E2_1581 at 1851.015 kb on + strand, within Pf6N2E2_1581 at 1851.015 kb on + strand, within Pf6N2E2_1581 at 1851.015 kb on + strand, within Pf6N2E2_1581 at 1851.016 kb on - strand, within Pf6N2E2_1581 at 1851.046 kb on - strand, within Pf6N2E2_1581 at 1851.066 kb on + strand, within Pf6N2E2_1581 at 1851.067 kb on - strand, within Pf6N2E2_1581 at 1851.204 kb on + strand, within Pf6N2E2_1581 at 1851.205 kb on - strand, within Pf6N2E2_1581 at 1851.344 kb on + strand at 1851.344 kb on + strand at 1851.344 kb on + strand at 1851.345 kb on - strand at 1851.345 kb on - strand at 1851.345 kb on - strand at 1851.432 kb on - strand at 1851.548 kb on + strand, within Pf6N2E2_1582 at 1851.549 kb on - strand, within Pf6N2E2_1582 at 1851.835 kb on - strand, within Pf6N2E2_1582 at 1851.881 kb on + strand, within Pf6N2E2_1582 at 1851.881 kb on + strand, within Pf6N2E2_1582 at 1852.118 kb on + strand, within Pf6N2E2_1582 at 1853.013 kb on + strand, within Pf6N2E2_1583 at 1853.013 kb on + strand, within Pf6N2E2_1583 at 1853.092 kb on - strand, within Pf6N2E2_1583 at 1853.547 kb on + strand, within Pf6N2E2_1583 at 1853.547 kb on + strand, within Pf6N2E2_1583 at 1853.566 kb on + strand, within Pf6N2E2_1583 at 1853.676 kb on + strand, within Pf6N2E2_1583
Per-strain Table
Position Strand Gene LocusTag Fraction bismuth(III) chloride 0.000025 M remove 1,850,415 + Pf6N2E2_1581 0.30 -1.1 1,850,415 + Pf6N2E2_1581 0.30 -2.8 1,850,435 + Pf6N2E2_1581 0.31 -0.2 1,850,435 + Pf6N2E2_1581 0.31 +1.0 1,850,448 + Pf6N2E2_1581 0.32 +0.4 1,850,448 + Pf6N2E2_1581 0.32 +0.0 1,850,448 + Pf6N2E2_1581 0.32 -0.3 1,850,448 + Pf6N2E2_1581 0.32 +0.3 1,850,448 + Pf6N2E2_1581 0.32 +0.8 1,850,448 + Pf6N2E2_1581 0.32 +0.9 1,850,449 - Pf6N2E2_1581 0.32 -0.0 1,850,449 - Pf6N2E2_1581 0.32 +0.4 1,850,449 - Pf6N2E2_1581 0.32 -0.3 1,850,481 + Pf6N2E2_1581 0.35 -1.1 1,850,835 + Pf6N2E2_1581 0.61 -1.5 1,850,835 + Pf6N2E2_1581 0.61 +0.7 1,850,928 + Pf6N2E2_1581 0.67 -0.4 1,850,928 + Pf6N2E2_1581 0.67 +0.6 1,850,928 + Pf6N2E2_1581 0.67 +0.1 1,851,015 + Pf6N2E2_1581 0.74 -0.4 1,851,015 + Pf6N2E2_1581 0.74 +0.4 1,851,015 + Pf6N2E2_1581 0.74 -0.3 1,851,016 - Pf6N2E2_1581 0.74 -0.9 1,851,046 - Pf6N2E2_1581 0.76 -0.8 1,851,066 + Pf6N2E2_1581 0.77 +0.1 1,851,067 - Pf6N2E2_1581 0.77 +0.4 1,851,204 + Pf6N2E2_1581 0.87 +0.5 1,851,205 - Pf6N2E2_1581 0.87 +0.3 1,851,344 + +0.1 1,851,344 + -1.4 1,851,344 + -0.7 1,851,345 - +0.4 1,851,345 - -1.7 1,851,345 - +1.3 1,851,432 - +1.5 1,851,548 + Pf6N2E2_1582 0.13 -1.1 1,851,549 - Pf6N2E2_1582 0.13 -0.1 1,851,835 - Pf6N2E2_1582 0.35 -0.8 1,851,881 + Pf6N2E2_1582 0.38 +1.5 1,851,881 + Pf6N2E2_1582 0.38 -0.8 1,852,118 + Pf6N2E2_1582 0.56 -0.9 1,853,013 + Pf6N2E2_1583 0.19 -0.0 1,853,013 + Pf6N2E2_1583 0.19 -0.1 1,853,092 - Pf6N2E2_1583 0.23 +0.3 1,853,547 + Pf6N2E2_1583 0.49 +0.7 1,853,547 + Pf6N2E2_1583 0.49 +0.3 1,853,566 + Pf6N2E2_1583 0.50 -1.3 1,853,676 + Pf6N2E2_1583 0.56 +0.1
Or see this region's nucleotide sequence