Experiment: Varel_Bryant medium with Dimethyl Sulfoxide 2 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2541 and BT2542 are separated by 7 nucleotides BT2542 and BT2543 are separated by 10 nucleotides
BT2541: BT2541 - putative calcium-transporting ATPase (NCBI ptt file), at 3,173,813 to 3,176,503
BT2541
BT2542: BT2542 - putative haloacid dehalogenase-like hydrolase (NCBI ptt file), at 3,176,511 to 3,177,134
BT2542
BT2543: BT2543 - riboflavin biosynthesis protein ribF, putative riboflavin kinase (NCBI ptt file), at 3,177,145 to 3,178,107
BT2543
Position (kb)
3176
3177
3178 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3175.555 kb on + strand, within BT2541 at 3175.876 kb on + strand, within BT2541 at 3175.899 kb on + strand, within BT2541 at 3175.923 kb on + strand, within BT2541 at 3175.941 kb on + strand, within BT2541 at 3175.941 kb on + strand, within BT2541 at 3176.014 kb on + strand, within BT2541 at 3176.095 kb on + strand, within BT2541 at 3176.095 kb on + strand, within BT2541 at 3176.122 kb on + strand, within BT2541 at 3176.127 kb on + strand, within BT2541 at 3176.171 kb on + strand, within BT2541 at 3176.197 kb on + strand, within BT2541 at 3176.209 kb on + strand, within BT2541 at 3176.209 kb on + strand, within BT2541 at 3176.209 kb on + strand, within BT2541 at 3176.431 kb on + strand at 3176.431 kb on + strand at 3176.488 kb on + strand at 3176.528 kb on + strand at 3176.557 kb on + strand at 3176.585 kb on + strand, within BT2542 at 3176.656 kb on + strand, within BT2542 at 3176.900 kb on + strand, within BT2542 at 3176.900 kb on + strand, within BT2542 at 3176.900 kb on + strand, within BT2542 at 3176.919 kb on + strand, within BT2542 at 3176.934 kb on + strand, within BT2542 at 3176.934 kb on + strand, within BT2542 at 3176.977 kb on + strand, within BT2542 at 3177.053 kb on + strand, within BT2542 at 3177.074 kb on + strand at 3177.140 kb on + strand at 3177.144 kb on + strand at 3178.082 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Dimethyl Sulfoxide 2 vol% remove 3,175,555 + BT2541 0.65 -1.3 3,175,876 + BT2541 0.77 -1.2 3,175,899 + BT2541 0.78 -0.3 3,175,923 + BT2541 0.78 -1.1 3,175,941 + BT2541 0.79 -1.3 3,175,941 + BT2541 0.79 +2.0 3,176,014 + BT2541 0.82 -3.1 3,176,095 + BT2541 0.85 +1.0 3,176,095 + BT2541 0.85 +0.0 3,176,122 + BT2541 0.86 +0.0 3,176,127 + BT2541 0.86 +0.0 3,176,171 + BT2541 0.88 +1.0 3,176,197 + BT2541 0.89 -1.3 3,176,209 + BT2541 0.89 +0.0 3,176,209 + BT2541 0.89 -1.0 3,176,209 + BT2541 0.89 +0.0 3,176,431 + -2.4 3,176,431 + -1.7 3,176,488 + -1.5 3,176,528 + -0.3 3,176,557 + -0.5 3,176,585 + BT2542 0.12 -1.0 3,176,656 + BT2542 0.23 -2.4 3,176,900 + BT2542 0.62 -1.1 3,176,900 + BT2542 0.62 +0.0 3,176,900 + BT2542 0.62 -0.5 3,176,919 + BT2542 0.65 +1.0 3,176,934 + BT2542 0.68 -0.5 3,176,934 + BT2542 0.68 +0.5 3,176,977 + BT2542 0.75 -0.3 3,177,053 + BT2542 0.87 -0.9 3,177,074 + -1.0 3,177,140 + -1.2 3,177,144 + +0.7 3,178,082 + -1.8
Or see this region's nucleotide sequence