Experiment: Europium(III) chloride 0.000195313 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1831 and Pf6N2E2_1832 overlap by 4 nucleotides Pf6N2E2_1832 and Pf6N2E2_1833 overlap by 8 nucleotides Pf6N2E2_1833 and Pf6N2E2_1834 are separated by 2 nucleotides Pf6N2E2_1834 and Pf6N2E2_1835 overlap by 4 nucleotides
Pf6N2E2_1831 - Citrate lyase beta chain (EC 4.1.3.6), at 2,169,405 to 2,170,304
_1831
Pf6N2E2_1832 - COGs COG3777, at 2,170,301 to 2,171,158
_1832
Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668
_1833
Pf6N2E2_1834 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,172,671 to 2,173,441
_1834
Pf6N2E2_1835 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,173,438 to 2,174,049
_1835
Position (kb)
2171
2172
2173 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2170.253 kb on + strand at 2170.406 kb on + strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.407 kb on - strand, within Pf6N2E2_1832 at 2170.463 kb on + strand, within Pf6N2E2_1832 at 2170.500 kb on - strand, within Pf6N2E2_1832 at 2170.572 kb on + strand, within Pf6N2E2_1832 at 2170.649 kb on - strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.903 kb on + strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.904 kb on - strand, within Pf6N2E2_1832 at 2170.996 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.036 kb on - strand, within Pf6N2E2_1832 at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.136 kb on + strand at 2171.137 kb on - strand at 2171.181 kb on + strand at 2171.655 kb on + strand, within Pf6N2E2_1833 at 2171.655 kb on + strand, within Pf6N2E2_1833 at 2171.657 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2171.926 kb on + strand, within Pf6N2E2_1833 at 2172.079 kb on + strand, within Pf6N2E2_1833 at 2172.081 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.156 kb on + strand, within Pf6N2E2_1833 at 2172.317 kb on + strand, within Pf6N2E2_1833 at 2172.317 kb on + strand, within Pf6N2E2_1833 at 2172.318 kb on - strand, within Pf6N2E2_1833 at 2172.318 kb on - strand, within Pf6N2E2_1833 at 2172.506 kb on + strand, within Pf6N2E2_1833 at 2172.574 kb on + strand at 2172.575 kb on - strand at 2172.575 kb on - strand at 2172.663 kb on - strand at 2172.668 kb on + strand at 2172.668 kb on + strand at 2172.669 kb on - strand at 2172.669 kb on - strand at 2172.672 kb on + strand at 2172.673 kb on - strand at 2173.007 kb on + strand, within Pf6N2E2_1834 at 2173.239 kb on - strand, within Pf6N2E2_1834 at 2173.399 kb on + strand at 2173.442 kb on - strand at 2173.442 kb on - strand at 2173.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Europium(III) chloride 0.000195313 M remove 2,170,253 + +2.4 2,170,406 + Pf6N2E2_1832 0.12 -0.1 2,170,407 - Pf6N2E2_1832 0.12 +0.5 2,170,407 - Pf6N2E2_1832 0.12 -1.4 2,170,407 - Pf6N2E2_1832 0.12 +0.2 2,170,463 + Pf6N2E2_1832 0.19 -0.5 2,170,500 - Pf6N2E2_1832 0.23 +1.1 2,170,572 + Pf6N2E2_1832 0.32 -0.4 2,170,649 - Pf6N2E2_1832 0.41 +0.9 2,170,903 + Pf6N2E2_1832 0.70 +0.2 2,170,903 + Pf6N2E2_1832 0.70 +0.7 2,170,903 + Pf6N2E2_1832 0.70 +0.2 2,170,903 + Pf6N2E2_1832 0.70 +0.6 2,170,903 + Pf6N2E2_1832 0.70 -0.8 2,170,903 + Pf6N2E2_1832 0.70 -2.4 2,170,903 + Pf6N2E2_1832 0.70 -0.6 2,170,904 - Pf6N2E2_1832 0.70 +0.7 2,170,904 - Pf6N2E2_1832 0.70 -1.2 2,170,996 - Pf6N2E2_1832 0.81 -0.6 2,171,036 - Pf6N2E2_1832 0.86 +0.5 2,171,036 - Pf6N2E2_1832 0.86 +0.1 2,171,136 + +1.1 2,171,136 + -0.4 2,171,136 + +0.4 2,171,137 - +0.1 2,171,181 + +0.7 2,171,655 + Pf6N2E2_1833 0.33 +0.7 2,171,655 + Pf6N2E2_1833 0.33 -0.1 2,171,657 + Pf6N2E2_1833 0.33 -1.5 2,171,926 + Pf6N2E2_1833 0.51 -0.0 2,171,926 + Pf6N2E2_1833 0.51 -0.6 2,172,079 + Pf6N2E2_1833 0.61 -0.6 2,172,081 + Pf6N2E2_1833 0.61 -1.0 2,172,156 + Pf6N2E2_1833 0.66 -0.8 2,172,156 + Pf6N2E2_1833 0.66 -0.6 2,172,317 + Pf6N2E2_1833 0.77 -0.3 2,172,317 + Pf6N2E2_1833 0.77 -2.0 2,172,318 - Pf6N2E2_1833 0.77 -0.1 2,172,318 - Pf6N2E2_1833 0.77 -1.6 2,172,506 + Pf6N2E2_1833 0.89 -0.4 2,172,574 + -0.1 2,172,575 - -0.6 2,172,575 - -0.0 2,172,663 - -0.2 2,172,668 + +0.8 2,172,668 + -0.4 2,172,669 - -0.7 2,172,669 - -0.0 2,172,672 + +0.7 2,172,673 - -0.8 2,173,007 + Pf6N2E2_1834 0.44 -0.4 2,173,239 - Pf6N2E2_1834 0.74 +0.2 2,173,399 + +0.8 2,173,442 - +0.3 2,173,442 - -1.3 2,173,448 + +0.2
Or see this region's nucleotide sequence