Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1833

Experiment: Europium(III) chloride 0.000195313 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1831 and Pf6N2E2_1832 overlap by 4 nucleotidesPf6N2E2_1832 and Pf6N2E2_1833 overlap by 8 nucleotidesPf6N2E2_1833 and Pf6N2E2_1834 are separated by 2 nucleotidesPf6N2E2_1834 and Pf6N2E2_1835 overlap by 4 nucleotides Pf6N2E2_1831 - Citrate lyase beta chain (EC 4.1.3.6), at 2,169,405 to 2,170,304 _1831 Pf6N2E2_1832 - COGs COG3777, at 2,170,301 to 2,171,158 _1832 Pf6N2E2_1833 - 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17), at 2,171,151 to 2,172,668 _1833 Pf6N2E2_1834 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,172,671 to 2,173,441 _1834 Pf6N2E2_1835 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,173,438 to 2,174,049 _1835 Position (kb) 2171 2172 2173Strain fitness (log2 ratio) -2 -1 0 1 2at 2170.253 kb on + strandat 2170.406 kb on + strand, within Pf6N2E2_1832at 2170.407 kb on - strand, within Pf6N2E2_1832at 2170.407 kb on - strand, within Pf6N2E2_1832at 2170.407 kb on - strand, within Pf6N2E2_1832at 2170.463 kb on + strand, within Pf6N2E2_1832at 2170.500 kb on - strand, within Pf6N2E2_1832at 2170.572 kb on + strand, within Pf6N2E2_1832at 2170.649 kb on - strand, within Pf6N2E2_1832at 2170.903 kb on + strand, within Pf6N2E2_1832at 2170.903 kb on + strand, within Pf6N2E2_1832at 2170.903 kb on + strand, within Pf6N2E2_1832at 2170.903 kb on + strand, within Pf6N2E2_1832at 2170.903 kb on + strand, within Pf6N2E2_1832at 2170.903 kb on + strand, within Pf6N2E2_1832at 2170.903 kb on + strand, within Pf6N2E2_1832at 2170.904 kb on - strand, within Pf6N2E2_1832at 2170.904 kb on - strand, within Pf6N2E2_1832at 2170.996 kb on - strand, within Pf6N2E2_1832at 2171.036 kb on - strand, within Pf6N2E2_1832at 2171.036 kb on - strand, within Pf6N2E2_1832at 2171.136 kb on + strandat 2171.136 kb on + strandat 2171.136 kb on + strandat 2171.137 kb on - strandat 2171.181 kb on + strandat 2171.655 kb on + strand, within Pf6N2E2_1833at 2171.655 kb on + strand, within Pf6N2E2_1833at 2171.657 kb on + strand, within Pf6N2E2_1833at 2171.926 kb on + strand, within Pf6N2E2_1833at 2171.926 kb on + strand, within Pf6N2E2_1833at 2172.079 kb on + strand, within Pf6N2E2_1833at 2172.081 kb on + strand, within Pf6N2E2_1833at 2172.156 kb on + strand, within Pf6N2E2_1833at 2172.156 kb on + strand, within Pf6N2E2_1833at 2172.317 kb on + strand, within Pf6N2E2_1833at 2172.317 kb on + strand, within Pf6N2E2_1833at 2172.318 kb on - strand, within Pf6N2E2_1833at 2172.318 kb on - strand, within Pf6N2E2_1833at 2172.506 kb on + strand, within Pf6N2E2_1833at 2172.574 kb on + strandat 2172.575 kb on - strandat 2172.575 kb on - strandat 2172.663 kb on - strandat 2172.668 kb on + strandat 2172.668 kb on + strandat 2172.669 kb on - strandat 2172.669 kb on - strandat 2172.672 kb on + strandat 2172.673 kb on - strandat 2173.007 kb on + strand, within Pf6N2E2_1834at 2173.239 kb on - strand, within Pf6N2E2_1834at 2173.399 kb on + strandat 2173.442 kb on - strandat 2173.442 kb on - strandat 2173.448 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Europium(III) chloride 0.000195313 M
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2,170,253 + +2.4
2,170,406 + Pf6N2E2_1832 0.12 -0.1
2,170,407 - Pf6N2E2_1832 0.12 +0.5
2,170,407 - Pf6N2E2_1832 0.12 -1.4
2,170,407 - Pf6N2E2_1832 0.12 +0.2
2,170,463 + Pf6N2E2_1832 0.19 -0.5
2,170,500 - Pf6N2E2_1832 0.23 +1.1
2,170,572 + Pf6N2E2_1832 0.32 -0.4
2,170,649 - Pf6N2E2_1832 0.41 +0.9
2,170,903 + Pf6N2E2_1832 0.70 +0.2
2,170,903 + Pf6N2E2_1832 0.70 +0.7
2,170,903 + Pf6N2E2_1832 0.70 +0.2
2,170,903 + Pf6N2E2_1832 0.70 +0.6
2,170,903 + Pf6N2E2_1832 0.70 -0.8
2,170,903 + Pf6N2E2_1832 0.70 -2.4
2,170,903 + Pf6N2E2_1832 0.70 -0.6
2,170,904 - Pf6N2E2_1832 0.70 +0.7
2,170,904 - Pf6N2E2_1832 0.70 -1.2
2,170,996 - Pf6N2E2_1832 0.81 -0.6
2,171,036 - Pf6N2E2_1832 0.86 +0.5
2,171,036 - Pf6N2E2_1832 0.86 +0.1
2,171,136 + +1.1
2,171,136 + -0.4
2,171,136 + +0.4
2,171,137 - +0.1
2,171,181 + +0.7
2,171,655 + Pf6N2E2_1833 0.33 +0.7
2,171,655 + Pf6N2E2_1833 0.33 -0.1
2,171,657 + Pf6N2E2_1833 0.33 -1.5
2,171,926 + Pf6N2E2_1833 0.51 -0.0
2,171,926 + Pf6N2E2_1833 0.51 -0.6
2,172,079 + Pf6N2E2_1833 0.61 -0.6
2,172,081 + Pf6N2E2_1833 0.61 -1.0
2,172,156 + Pf6N2E2_1833 0.66 -0.8
2,172,156 + Pf6N2E2_1833 0.66 -0.6
2,172,317 + Pf6N2E2_1833 0.77 -0.3
2,172,317 + Pf6N2E2_1833 0.77 -2.0
2,172,318 - Pf6N2E2_1833 0.77 -0.1
2,172,318 - Pf6N2E2_1833 0.77 -1.6
2,172,506 + Pf6N2E2_1833 0.89 -0.4
2,172,574 + -0.1
2,172,575 - -0.6
2,172,575 - -0.0
2,172,663 - -0.2
2,172,668 + +0.8
2,172,668 + -0.4
2,172,669 - -0.7
2,172,669 - -0.0
2,172,672 + +0.7
2,172,673 - -0.8
2,173,007 + Pf6N2E2_1834 0.44 -0.4
2,173,239 - Pf6N2E2_1834 0.74 +0.2
2,173,399 + +0.8
2,173,442 - +0.3
2,173,442 - -1.3
2,173,448 + +0.2

Or see this region's nucleotide sequence