Experiment: Protocatechuic Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_04593 and H281DRAFT_04594 are separated by 10 nucleotides H281DRAFT_04594 and H281DRAFT_04595 overlap by 4 nucleotides H281DRAFT_04595 and H281DRAFT_04596 are separated by 122 nucleotides H281DRAFT_04596 and H281DRAFT_04597 are separated by 121 nucleotides
H281DRAFT_04593: H281DRAFT_04593 - probable phosphoglycerate mutase, at 325,067 to 325,819
_04593
H281DRAFT_04594: H281DRAFT_04594 - putative oxepin-CoA hydrolase (EC 3.3.2.12) <small>(from data)</small>, at 325,830 to 326,618
_04594
H281DRAFT_04595: H281DRAFT_04595 - 3-hydroxyacyl-CoA dehydrogenase, at 326,615 to 328,180
_04595
H281DRAFT_04596: H281DRAFT_04596 - glutathione S-transferase, at 328,303 to 328,950
_04596
H281DRAFT_04597: H281DRAFT_04597 - 2-keto-4-methylthiobutyrate aminotransferase apoenzyme, at 329,072 to 330,244
_04597
Position (kb) 326
327
328
329 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 325.719 kb on + strand, within H281DRAFT_04593 at 325.719 kb on + strand, within H281DRAFT_04593 at 325.719 kb on + strand, within H281DRAFT_04593 at 325.743 kb on - strand, within H281DRAFT_04593 at 325.762 kb on + strand at 325.766 kb on + strand at 325.773 kb on + strand at 325.773 kb on + strand at 325.773 kb on + strand at 325.774 kb on - strand at 325.835 kb on + strand at 325.837 kb on + strand at 325.913 kb on + strand, within H281DRAFT_04594 at 326.001 kb on + strand, within H281DRAFT_04594 at 326.515 kb on + strand, within H281DRAFT_04594 at 326.516 kb on - strand, within H281DRAFT_04594 at 326.516 kb on - strand, within H281DRAFT_04594 at 326.565 kb on + strand at 326.746 kb on + strand at 326.902 kb on + strand, within H281DRAFT_04595 at 327.032 kb on - strand, within H281DRAFT_04595 at 327.225 kb on + strand, within H281DRAFT_04595 at 327.226 kb on - strand, within H281DRAFT_04595 at 327.319 kb on - strand, within H281DRAFT_04595 at 327.324 kb on + strand, within H281DRAFT_04595 at 327.382 kb on + strand, within H281DRAFT_04595 at 327.383 kb on - strand, within H281DRAFT_04595 at 327.395 kb on - strand, within H281DRAFT_04595 at 327.411 kb on + strand, within H281DRAFT_04595 at 327.423 kb on + strand, within H281DRAFT_04595 at 327.424 kb on - strand, within H281DRAFT_04595 at 327.449 kb on + strand, within H281DRAFT_04595 at 327.665 kb on + strand, within H281DRAFT_04595 at 327.867 kb on - strand, within H281DRAFT_04595 at 327.867 kb on - strand, within H281DRAFT_04595 at 328.032 kb on + strand at 328.033 kb on - strand at 328.072 kb on - strand at 328.157 kb on + strand at 328.158 kb on - strand at 328.202 kb on + strand at 328.213 kb on - strand at 328.333 kb on - strand at 328.336 kb on + strand at 328.336 kb on + strand at 328.337 kb on - strand at 328.337 kb on - strand at 328.339 kb on + strand at 328.340 kb on - strand at 328.340 kb on - strand at 328.340 kb on - strand at 328.397 kb on + strand, within H281DRAFT_04596 at 328.494 kb on + strand, within H281DRAFT_04596 at 328.494 kb on + strand, within H281DRAFT_04596 at 328.494 kb on + strand, within H281DRAFT_04596 at 328.494 kb on + strand, within H281DRAFT_04596 at 328.495 kb on - strand, within H281DRAFT_04596 at 328.495 kb on - strand, within H281DRAFT_04596 at 328.504 kb on + strand, within H281DRAFT_04596 at 328.819 kb on + strand, within H281DRAFT_04596 at 328.864 kb on + strand, within H281DRAFT_04596 at 328.864 kb on + strand, within H281DRAFT_04596 at 328.864 kb on + strand, within H281DRAFT_04596 at 328.864 kb on + strand, within H281DRAFT_04596 at 328.924 kb on + strand at 328.925 kb on - strand at 329.073 kb on + strand at 329.074 kb on - strand at 329.074 kb on - strand at 329.138 kb on + strand at 329.138 kb on + strand at 329.138 kb on + strand at 329.139 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Protocatechuic Acid (C) remove 325,719 + H281DRAFT_04593 0.87 +0.7 325,719 + H281DRAFT_04593 0.87 +0.2 325,719 + H281DRAFT_04593 0.87 -0.7 325,743 - H281DRAFT_04593 0.90 +0.9 325,762 + +1.2 325,766 + +0.0 325,773 + +0.6 325,773 + +0.5 325,773 + -0.3 325,774 - -2.8 325,835 + +0.6 325,837 + -0.2 325,913 + H281DRAFT_04594 0.11 -1.6 326,001 + H281DRAFT_04594 0.22 -0.2 326,515 + H281DRAFT_04594 0.87 -0.1 326,516 - H281DRAFT_04594 0.87 +1.0 326,516 - H281DRAFT_04594 0.87 -0.2 326,565 + +0.7 326,746 + +0.4 326,902 + H281DRAFT_04595 0.18 -0.2 327,032 - H281DRAFT_04595 0.27 +0.8 327,225 + H281DRAFT_04595 0.39 +1.0 327,226 - H281DRAFT_04595 0.39 +0.2 327,319 - H281DRAFT_04595 0.45 +0.4 327,324 + H281DRAFT_04595 0.45 +0.6 327,382 + H281DRAFT_04595 0.49 +0.2 327,383 - H281DRAFT_04595 0.49 +1.1 327,395 - H281DRAFT_04595 0.50 +0.4 327,411 + H281DRAFT_04595 0.51 -0.2 327,423 + H281DRAFT_04595 0.52 +0.9 327,424 - H281DRAFT_04595 0.52 +1.0 327,449 + H281DRAFT_04595 0.53 +0.5 327,665 + H281DRAFT_04595 0.67 +0.3 327,867 - H281DRAFT_04595 0.80 -1.2 327,867 - H281DRAFT_04595 0.80 -0.5 328,032 + +1.9 328,033 - +0.8 328,072 - -1.0 328,157 + -0.1 328,158 - +1.1 328,202 + +0.3 328,213 - -1.4 328,333 - +1.2 328,336 + +0.2 328,336 + +0.0 328,337 - +0.2 328,337 - +0.5 328,339 + +0.9 328,340 - +0.8 328,340 - +0.1 328,340 - -0.3 328,397 + H281DRAFT_04596 0.15 +0.1 328,494 + H281DRAFT_04596 0.29 -0.1 328,494 + H281DRAFT_04596 0.29 -1.9 328,494 + H281DRAFT_04596 0.29 +0.4 328,494 + H281DRAFT_04596 0.29 -0.3 328,495 - H281DRAFT_04596 0.30 +0.7 328,495 - H281DRAFT_04596 0.30 +0.6 328,504 + H281DRAFT_04596 0.31 -1.0 328,819 + H281DRAFT_04596 0.80 -0.8 328,864 + H281DRAFT_04596 0.87 -1.0 328,864 + H281DRAFT_04596 0.87 -0.4 328,864 + H281DRAFT_04596 0.87 +0.6 328,864 + H281DRAFT_04596 0.87 -1.1 328,924 + -2.6 328,925 - +1.3 329,073 + +0.0 329,074 - +0.2 329,074 - +0.5 329,138 + +0.2 329,138 + +0.2 329,138 + +0.8 329,139 - +0.1
Or see this region's nucleotide sequence