Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05330

Experiment: copper (II) chloride 3 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05315 and BWI76_RS05320 overlap by 14 nucleotidesBWI76_RS05320 and BWI76_RS05325 are separated by 196 nucleotidesBWI76_RS05325 and BWI76_RS05330 overlap by 4 nucleotidesBWI76_RS05330 and BWI76_RS05335 are separated by 20 nucleotidesBWI76_RS05335 and BWI76_RS05340 are separated by 64 nucleotides BWI76_RS05315: BWI76_RS05315 - Rho-binding antiterminator, at 1,121,696 to 1,121,956 _RS05315 BWI76_RS05320: BWI76_RS05320 - hypothetical protein, at 1,121,943 to 1,122,143 _RS05320 BWI76_RS05325: BWI76_RS05325 - hypothetical protein, at 1,122,340 to 1,122,885 _RS05325 BWI76_RS05330: BWI76_RS05330 - aminoacyl-tRNA hydrolase, at 1,122,882 to 1,123,295 _RS05330 BWI76_RS05335: BWI76_RS05335 - copper homeostasis/adhesion lipoprotein NlpE, at 1,123,316 to 1,124,035 _RS05335 BWI76_RS05340: BWI76_RS05340 - proline--tRNA ligase, at 1,124,100 to 1,125,818 _RS05340 Position (kb) 1122 1123 1124Strain fitness (log2 ratio) -3 -2 -1 0 1at 1122.193 kb on - strandat 1122.206 kb on - strandat 1122.219 kb on + strandat 1122.463 kb on - strand, within BWI76_RS05325at 1122.595 kb on - strand, within BWI76_RS05325at 1122.743 kb on - strand, within BWI76_RS05325at 1122.754 kb on - strand, within BWI76_RS05325at 1122.762 kb on + strand, within BWI76_RS05325at 1122.813 kb on - strand, within BWI76_RS05325at 1122.813 kb on - strand, within BWI76_RS05325at 1123.014 kb on + strand, within BWI76_RS05330at 1123.167 kb on - strand, within BWI76_RS05330at 1123.214 kb on - strand, within BWI76_RS05330at 1123.296 kb on + strandat 1123.569 kb on + strand, within BWI76_RS05335at 1123.875 kb on - strand, within BWI76_RS05335at 1124.023 kb on - strandat 1124.058 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction copper (II) chloride 3 mM
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1,122,193 - -0.4
1,122,206 - +0.3
1,122,219 + +0.6
1,122,463 - BWI76_RS05325 0.23 +0.2
1,122,595 - BWI76_RS05325 0.47 -1.1
1,122,743 - BWI76_RS05325 0.74 -0.4
1,122,754 - BWI76_RS05325 0.76 -2.7
1,122,762 + BWI76_RS05325 0.77 -0.3
1,122,813 - BWI76_RS05325 0.87 -1.9
1,122,813 - BWI76_RS05325 0.87 -1.9
1,123,014 + BWI76_RS05330 0.32 +0.1
1,123,167 - BWI76_RS05330 0.69 -2.2
1,123,214 - BWI76_RS05330 0.80 -2.8
1,123,296 + +0.8
1,123,569 + BWI76_RS05335 0.35 -3.3
1,123,875 - BWI76_RS05335 0.78 -0.4
1,124,023 - -1.4
1,124,058 + -1.5

Or see this region's nucleotide sequence