Experiment: MRS + kanamycin Time5hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14955 and BBR_RS14960 are separated by 526 nucleotides BBR_RS14960 and BBR_RS14965 are separated by 140 nucleotides BBR_RS14965 and BBR_RS14970 are separated by 6 nucleotides BBR_RS14970 and BBR_RS14975 are separated by 129 nucleotides
BBR_RS14955: BBR_RS14955 - hypothetical protein, at 1,129,131 to 1,130,063
_RS14955
BBR_RS14960: BBR_RS14960 - alpha/beta hydrolase, at 1,130,590 to 1,131,411
_RS14960
BBR_RS14965: BBR_RS14965 - DUF2975 domain-containing protein, at 1,131,552 to 1,131,812
_RS14965
BBR_RS14970: BBR_RS14970 - transcriptional regulator, at 1,131,819 to 1,132,028
_RS14970
BBR_RS14975: BBR_RS14975 - purine permease, at 1,132,158 to 1,133,522
_RS14975
Position (kb)
1130
1131
1132 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1129.638 kb on + strand, within BBR_RS14955 at 1129.677 kb on + strand, within BBR_RS14955 at 1129.709 kb on + strand, within BBR_RS14955 at 1129.732 kb on + strand, within BBR_RS14955 at 1129.765 kb on + strand, within BBR_RS14955 at 1129.802 kb on - strand, within BBR_RS14955 at 1129.833 kb on - strand, within BBR_RS14955 at 1129.833 kb on - strand, within BBR_RS14955 at 1129.840 kb on + strand, within BBR_RS14955 at 1129.861 kb on + strand, within BBR_RS14955 at 1130.050 kb on - strand at 1130.128 kb on + strand at 1130.168 kb on - strand at 1130.180 kb on - strand at 1130.209 kb on + strand at 1130.263 kb on - strand at 1130.400 kb on + strand at 1130.546 kb on + strand at 1130.559 kb on - strand at 1130.596 kb on - strand at 1130.615 kb on - strand at 1130.658 kb on + strand at 1130.658 kb on + strand at 1130.660 kb on + strand at 1130.661 kb on - strand at 1130.701 kb on + strand, within BBR_RS14960 at 1130.734 kb on + strand, within BBR_RS14960 at 1130.735 kb on + strand, within BBR_RS14960 at 1130.743 kb on - strand, within BBR_RS14960 at 1130.770 kb on - strand, within BBR_RS14960 at 1131.051 kb on + strand, within BBR_RS14960 at 1131.059 kb on + strand, within BBR_RS14960 at 1131.096 kb on + strand, within BBR_RS14960 at 1131.135 kb on - strand, within BBR_RS14960 at 1131.137 kb on - strand, within BBR_RS14960 at 1131.164 kb on + strand, within BBR_RS14960 at 1131.170 kb on - strand, within BBR_RS14960 at 1131.217 kb on + strand, within BBR_RS14960 at 1131.230 kb on - strand, within BBR_RS14960 at 1131.270 kb on - strand, within BBR_RS14960 at 1131.287 kb on + strand, within BBR_RS14960 at 1131.312 kb on + strand, within BBR_RS14960 at 1131.320 kb on - strand, within BBR_RS14960 at 1131.345 kb on + strand at 1131.400 kb on + strand at 1131.424 kb on + strand at 1131.424 kb on + strand at 1131.426 kb on - strand at 1131.467 kb on + strand at 1131.550 kb on - strand at 1131.574 kb on - strand at 1131.638 kb on + strand, within BBR_RS14965 at 1131.668 kb on + strand, within BBR_RS14965 at 1131.668 kb on + strand, within BBR_RS14965 at 1131.668 kb on + strand, within BBR_RS14965 at 1131.669 kb on - strand, within BBR_RS14965 at 1131.703 kb on + strand, within BBR_RS14965 at 1131.713 kb on + strand, within BBR_RS14965 at 1131.720 kb on + strand, within BBR_RS14965 at 1131.747 kb on - strand, within BBR_RS14965 at 1131.760 kb on - strand, within BBR_RS14965 at 1131.795 kb on - strand at 1131.935 kb on + strand, within BBR_RS14970 at 1131.943 kb on - strand, within BBR_RS14970 at 1131.950 kb on + strand, within BBR_RS14970 at 1131.978 kb on + strand, within BBR_RS14970 at 1131.978 kb on + strand, within BBR_RS14970 at 1131.993 kb on + strand, within BBR_RS14970 at 1132.062 kb on + strand at 1132.121 kb on - strand at 1132.138 kb on + strand at 1132.159 kb on - strand at 1132.171 kb on - strand at 1132.186 kb on - strand at 1132.188 kb on - strand at 1132.210 kb on + strand at 1132.213 kb on - strand at 1132.218 kb on - strand at 1132.225 kb on + strand at 1132.225 kb on + strand at 1132.292 kb on + strand at 1132.300 kb on - strand, within BBR_RS14975 at 1132.302 kb on - strand, within BBR_RS14975 at 1132.323 kb on - strand, within BBR_RS14975 at 1132.340 kb on - strand, within BBR_RS14975
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + kanamycin Time5hr remove 1,129,638 + BBR_RS14955 0.54 -1.3 1,129,677 + BBR_RS14955 0.59 +1.6 1,129,709 + BBR_RS14955 0.62 +0.1 1,129,732 + BBR_RS14955 0.64 +0.6 1,129,765 + BBR_RS14955 0.68 +1.4 1,129,802 - BBR_RS14955 0.72 -0.4 1,129,833 - BBR_RS14955 0.75 +0.1 1,129,833 - BBR_RS14955 0.75 +1.6 1,129,840 + BBR_RS14955 0.76 +2.7 1,129,861 + BBR_RS14955 0.78 -1.8 1,130,050 - -1.5 1,130,128 + -3.5 1,130,168 - -1.1 1,130,180 - +1.8 1,130,209 + -1.0 1,130,263 - -1.5 1,130,400 + -1.0 1,130,546 + -1.0 1,130,559 - -0.4 1,130,596 - +0.6 1,130,615 - -1.0 1,130,658 + +0.6 1,130,658 + +0.2 1,130,660 + +1.2 1,130,661 - +0.1 1,130,701 + BBR_RS14960 0.14 +0.7 1,130,734 + BBR_RS14960 0.18 +1.9 1,130,735 + BBR_RS14960 0.18 +1.3 1,130,743 - BBR_RS14960 0.19 +1.3 1,130,770 - BBR_RS14960 0.22 -0.0 1,131,051 + BBR_RS14960 0.56 +1.2 1,131,059 + BBR_RS14960 0.57 +0.6 1,131,096 + BBR_RS14960 0.62 +1.9 1,131,135 - BBR_RS14960 0.66 +1.0 1,131,137 - BBR_RS14960 0.67 +0.4 1,131,164 + BBR_RS14960 0.70 +1.4 1,131,170 - BBR_RS14960 0.71 -0.1 1,131,217 + BBR_RS14960 0.76 -1.4 1,131,230 - BBR_RS14960 0.78 +3.1 1,131,270 - BBR_RS14960 0.83 +1.0 1,131,287 + BBR_RS14960 0.85 +0.1 1,131,312 + BBR_RS14960 0.88 -0.6 1,131,320 - BBR_RS14960 0.89 +1.8 1,131,345 + +0.3 1,131,400 + -1.7 1,131,424 + -1.8 1,131,424 + +0.3 1,131,426 - +1.0 1,131,467 + +1.3 1,131,550 - -0.0 1,131,574 - +1.6 1,131,638 + BBR_RS14965 0.33 +0.3 1,131,668 + BBR_RS14965 0.44 -0.0 1,131,668 + BBR_RS14965 0.44 +0.6 1,131,668 + BBR_RS14965 0.44 -0.6 1,131,669 - BBR_RS14965 0.45 -1.0 1,131,703 + BBR_RS14965 0.58 -0.9 1,131,713 + BBR_RS14965 0.62 +1.0 1,131,720 + BBR_RS14965 0.64 +0.3 1,131,747 - BBR_RS14965 0.75 +1.8 1,131,760 - BBR_RS14965 0.80 -0.3 1,131,795 - +1.4 1,131,935 + BBR_RS14970 0.55 +1.3 1,131,943 - BBR_RS14970 0.59 -1.0 1,131,950 + BBR_RS14970 0.62 -0.0 1,131,978 + BBR_RS14970 0.76 -0.4 1,131,978 + BBR_RS14970 0.76 -0.6 1,131,993 + BBR_RS14970 0.83 -0.6 1,132,062 + -0.7 1,132,121 - -0.9 1,132,138 + -4.0 1,132,159 - -0.5 1,132,171 - +0.1 1,132,186 - -2.6 1,132,188 - -0.5 1,132,210 + +0.2 1,132,213 - -1.1 1,132,218 - +3.3 1,132,225 + +1.3 1,132,225 + +3.0 1,132,292 + +0.2 1,132,300 - BBR_RS14975 0.10 +0.6 1,132,302 - BBR_RS14975 0.11 -0.2 1,132,323 - BBR_RS14975 0.12 +2.0 1,132,340 - BBR_RS14975 0.13 +0.9
Or see this region's nucleotide sequence