Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS14960

Experiment: MRS + kanamycin Time5hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBBR_RS14955 and BBR_RS14960 are separated by 526 nucleotidesBBR_RS14960 and BBR_RS14965 are separated by 140 nucleotidesBBR_RS14965 and BBR_RS14970 are separated by 6 nucleotidesBBR_RS14970 and BBR_RS14975 are separated by 129 nucleotides BBR_RS14955: BBR_RS14955 - hypothetical protein, at 1,129,131 to 1,130,063 _RS14955 BBR_RS14960: BBR_RS14960 - alpha/beta hydrolase, at 1,130,590 to 1,131,411 _RS14960 BBR_RS14965: BBR_RS14965 - DUF2975 domain-containing protein, at 1,131,552 to 1,131,812 _RS14965 BBR_RS14970: BBR_RS14970 - transcriptional regulator, at 1,131,819 to 1,132,028 _RS14970 BBR_RS14975: BBR_RS14975 - purine permease, at 1,132,158 to 1,133,522 _RS14975 Position (kb) 1130 1131 1132Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1129.638 kb on + strand, within BBR_RS14955at 1129.677 kb on + strand, within BBR_RS14955at 1129.709 kb on + strand, within BBR_RS14955at 1129.732 kb on + strand, within BBR_RS14955at 1129.765 kb on + strand, within BBR_RS14955at 1129.802 kb on - strand, within BBR_RS14955at 1129.833 kb on - strand, within BBR_RS14955at 1129.833 kb on - strand, within BBR_RS14955at 1129.840 kb on + strand, within BBR_RS14955at 1129.861 kb on + strand, within BBR_RS14955at 1130.050 kb on - strandat 1130.128 kb on + strandat 1130.168 kb on - strandat 1130.180 kb on - strandat 1130.209 kb on + strandat 1130.263 kb on - strandat 1130.400 kb on + strandat 1130.546 kb on + strandat 1130.559 kb on - strandat 1130.596 kb on - strandat 1130.615 kb on - strandat 1130.658 kb on + strandat 1130.658 kb on + strandat 1130.660 kb on + strandat 1130.661 kb on - strandat 1130.701 kb on + strand, within BBR_RS14960at 1130.734 kb on + strand, within BBR_RS14960at 1130.735 kb on + strand, within BBR_RS14960at 1130.743 kb on - strand, within BBR_RS14960at 1130.770 kb on - strand, within BBR_RS14960at 1131.051 kb on + strand, within BBR_RS14960at 1131.059 kb on + strand, within BBR_RS14960at 1131.096 kb on + strand, within BBR_RS14960at 1131.135 kb on - strand, within BBR_RS14960at 1131.137 kb on - strand, within BBR_RS14960at 1131.164 kb on + strand, within BBR_RS14960at 1131.170 kb on - strand, within BBR_RS14960at 1131.217 kb on + strand, within BBR_RS14960at 1131.230 kb on - strand, within BBR_RS14960at 1131.270 kb on - strand, within BBR_RS14960at 1131.287 kb on + strand, within BBR_RS14960at 1131.312 kb on + strand, within BBR_RS14960at 1131.320 kb on - strand, within BBR_RS14960at 1131.345 kb on + strandat 1131.400 kb on + strandat 1131.424 kb on + strandat 1131.424 kb on + strandat 1131.426 kb on - strandat 1131.467 kb on + strandat 1131.550 kb on - strandat 1131.574 kb on - strandat 1131.638 kb on + strand, within BBR_RS14965at 1131.668 kb on + strand, within BBR_RS14965at 1131.668 kb on + strand, within BBR_RS14965at 1131.668 kb on + strand, within BBR_RS14965at 1131.669 kb on - strand, within BBR_RS14965at 1131.703 kb on + strand, within BBR_RS14965at 1131.713 kb on + strand, within BBR_RS14965at 1131.720 kb on + strand, within BBR_RS14965at 1131.747 kb on - strand, within BBR_RS14965at 1131.760 kb on - strand, within BBR_RS14965at 1131.795 kb on - strandat 1131.935 kb on + strand, within BBR_RS14970at 1131.943 kb on - strand, within BBR_RS14970at 1131.950 kb on + strand, within BBR_RS14970at 1131.978 kb on + strand, within BBR_RS14970at 1131.978 kb on + strand, within BBR_RS14970at 1131.993 kb on + strand, within BBR_RS14970at 1132.062 kb on + strandat 1132.121 kb on - strandat 1132.138 kb on + strandat 1132.159 kb on - strandat 1132.171 kb on - strandat 1132.186 kb on - strandat 1132.188 kb on - strandat 1132.210 kb on + strandat 1132.213 kb on - strandat 1132.218 kb on - strandat 1132.225 kb on + strandat 1132.225 kb on + strandat 1132.292 kb on + strandat 1132.300 kb on - strand, within BBR_RS14975at 1132.302 kb on - strand, within BBR_RS14975at 1132.323 kb on - strand, within BBR_RS14975at 1132.340 kb on - strand, within BBR_RS14975

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Per-strain Table

Position Strand Gene LocusTag Fraction MRS + kanamycin Time5hr
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1,129,638 + BBR_RS14955 0.54 -1.3
1,129,677 + BBR_RS14955 0.59 +1.6
1,129,709 + BBR_RS14955 0.62 +0.1
1,129,732 + BBR_RS14955 0.64 +0.6
1,129,765 + BBR_RS14955 0.68 +1.4
1,129,802 - BBR_RS14955 0.72 -0.4
1,129,833 - BBR_RS14955 0.75 +0.1
1,129,833 - BBR_RS14955 0.75 +1.6
1,129,840 + BBR_RS14955 0.76 +2.7
1,129,861 + BBR_RS14955 0.78 -1.8
1,130,050 - -1.5
1,130,128 + -3.5
1,130,168 - -1.1
1,130,180 - +1.8
1,130,209 + -1.0
1,130,263 - -1.5
1,130,400 + -1.0
1,130,546 + -1.0
1,130,559 - -0.4
1,130,596 - +0.6
1,130,615 - -1.0
1,130,658 + +0.6
1,130,658 + +0.2
1,130,660 + +1.2
1,130,661 - +0.1
1,130,701 + BBR_RS14960 0.14 +0.7
1,130,734 + BBR_RS14960 0.18 +1.9
1,130,735 + BBR_RS14960 0.18 +1.3
1,130,743 - BBR_RS14960 0.19 +1.3
1,130,770 - BBR_RS14960 0.22 -0.0
1,131,051 + BBR_RS14960 0.56 +1.2
1,131,059 + BBR_RS14960 0.57 +0.6
1,131,096 + BBR_RS14960 0.62 +1.9
1,131,135 - BBR_RS14960 0.66 +1.0
1,131,137 - BBR_RS14960 0.67 +0.4
1,131,164 + BBR_RS14960 0.70 +1.4
1,131,170 - BBR_RS14960 0.71 -0.1
1,131,217 + BBR_RS14960 0.76 -1.4
1,131,230 - BBR_RS14960 0.78 +3.1
1,131,270 - BBR_RS14960 0.83 +1.0
1,131,287 + BBR_RS14960 0.85 +0.1
1,131,312 + BBR_RS14960 0.88 -0.6
1,131,320 - BBR_RS14960 0.89 +1.8
1,131,345 + +0.3
1,131,400 + -1.7
1,131,424 + -1.8
1,131,424 + +0.3
1,131,426 - +1.0
1,131,467 + +1.3
1,131,550 - -0.0
1,131,574 - +1.6
1,131,638 + BBR_RS14965 0.33 +0.3
1,131,668 + BBR_RS14965 0.44 -0.0
1,131,668 + BBR_RS14965 0.44 +0.6
1,131,668 + BBR_RS14965 0.44 -0.6
1,131,669 - BBR_RS14965 0.45 -1.0
1,131,703 + BBR_RS14965 0.58 -0.9
1,131,713 + BBR_RS14965 0.62 +1.0
1,131,720 + BBR_RS14965 0.64 +0.3
1,131,747 - BBR_RS14965 0.75 +1.8
1,131,760 - BBR_RS14965 0.80 -0.3
1,131,795 - +1.4
1,131,935 + BBR_RS14970 0.55 +1.3
1,131,943 - BBR_RS14970 0.59 -1.0
1,131,950 + BBR_RS14970 0.62 -0.0
1,131,978 + BBR_RS14970 0.76 -0.4
1,131,978 + BBR_RS14970 0.76 -0.6
1,131,993 + BBR_RS14970 0.83 -0.6
1,132,062 + -0.7
1,132,121 - -0.9
1,132,138 + -4.0
1,132,159 - -0.5
1,132,171 - +0.1
1,132,186 - -2.6
1,132,188 - -0.5
1,132,210 + +0.2
1,132,213 - -1.1
1,132,218 - +3.3
1,132,225 + +1.3
1,132,225 + +3.0
1,132,292 + +0.2
1,132,300 - BBR_RS14975 0.10 +0.6
1,132,302 - BBR_RS14975 0.11 -0.2
1,132,323 - BBR_RS14975 0.12 +2.0
1,132,340 - BBR_RS14975 0.13 +0.9

Or see this region's nucleotide sequence