Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3199 and DvMF_3200 are separated by 54 nucleotides DvMF_3200 and DvMF_3201 are separated by 106 nucleotides DvMF_3201 and ruvC are separated by 268 nucleotides
DvMF_3199: DvMF_3199 - hypothetical protein (RefSeq), at 4,030,654 to 4,032,516
_3199
DvMF_3200: DvMF_3200 - ribosomal RNA methyltransferase RrmJ/FtsJ (RefSeq), at 4,032,571 to 4,033,176
_3200
DvMF_3201: DvMF_3201 - hypothetical protein (RefSeq), at 4,033,283 to 4,034,026
_3201
DvMF_3202: ruvC - Holliday junction resolvase (RefSeq), at 4,034,295 to 4,034,888
ruvC
Position (kb)
4033
4034
4035 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4032.334 kb on - strand at 4032.511 kb on + strand at 4032.568 kb on - strand at 4032.570 kb on + strand at 4032.842 kb on + strand, within DvMF_3200 at 4032.937 kb on + strand, within DvMF_3200 at 4033.002 kb on + strand, within DvMF_3200 at 4033.401 kb on - strand, within DvMF_3201 at 4033.855 kb on + strand, within DvMF_3201 at 4033.876 kb on + strand, within DvMF_3201 at 4034.004 kb on - strand at 4034.484 kb on - strand, within ruvC at 4034.509 kb on - strand, within ruvC at 4034.691 kb on - strand, within ruvC at 4034.772 kb on - strand, within ruvC at 4034.921 kb on + strand at 4034.929 kb on - strand at 4034.995 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 4,032,334 - +0.6 4,032,511 + -0.4 4,032,568 - -1.0 4,032,570 + -0.1 4,032,842 + DvMF_3200 0.45 -0.2 4,032,937 + DvMF_3200 0.60 -0.2 4,033,002 + DvMF_3200 0.71 -0.5 4,033,401 - DvMF_3201 0.16 +0.4 4,033,855 + DvMF_3201 0.77 -0.2 4,033,876 + DvMF_3201 0.80 +1.8 4,034,004 - -0.4 4,034,484 - ruvC DvMF_3202 0.32 -1.9 4,034,509 - ruvC DvMF_3202 0.36 -1.3 4,034,691 - ruvC DvMF_3202 0.67 -2.0 4,034,772 - ruvC DvMF_3202 0.80 -1.4 4,034,921 + -0.3 4,034,929 - -0.5 4,034,995 + +0.8
Or see this region's nucleotide sequence