Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2871 and DvMF_R0075 are separated by 518 nucleotides DvMF_R0075 and argJ are separated by 252 nucleotides argJ and DvMF_2873 are separated by 336 nucleotides
DvMF_2871: DvMF_2871 - MotA/TolQ/ExbB proton channel (RefSeq), at 3,623,621 to 3,624,376
_2871
DvMF_R0075: DvMF_R0075 - tRNA-Ser (RefSeq), at 3,624,895 to 3,624,987
_R0075
DvMF_2872: argJ - bifunctional ornithine acetyltransferase/N-acetylglutamate synthase protein (RefSeq), at 3,625,240 to 3,626,478
argJ
DvMF_2873: DvMF_2873 - preprotein translocase subunit SecA (RefSeq), at 3,626,815 to 3,629,349
_2873
Position (kb)
3625
3626
3627 Strain fitness (log2 ratio)
-10
-9
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 3624.749 kb on + strand at 3624.749 kb on + strand at 3624.803 kb on + strand at 3625.200 kb on - strand at 3625.307 kb on - strand at 3625.372 kb on - strand, within argJ at 3625.372 kb on - strand, within argJ at 3625.372 kb on - strand, within argJ at 3625.406 kb on + strand, within argJ at 3625.414 kb on - strand, within argJ at 3625.907 kb on + strand, within argJ at 3626.116 kb on - strand, within argJ at 3626.241 kb on - strand, within argJ at 3626.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 3,624,749 + -1.4 3,624,749 + -1.0 3,624,803 + -0.6 3,625,200 - +0.1 3,625,307 - -5.7 3,625,372 - argJ DvMF_2872 0.11 -5.6 3,625,372 - argJ DvMF_2872 0.11 -6.8 3,625,372 - argJ DvMF_2872 0.11 -4.4 3,625,406 + argJ DvMF_2872 0.13 -5.3 3,625,414 - argJ DvMF_2872 0.14 -9.8 3,625,907 + argJ DvMF_2872 0.54 -3.2 3,626,116 - argJ DvMF_2872 0.71 -7.1 3,626,241 - argJ DvMF_2872 0.81 -4.4 3,626,808 - -0.0
Or see this region's nucleotide sequence