Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2293 and DvMF_2294 are separated by 649 nucleotides DvMF_2294 and DvMF_2295 are separated by 6 nucleotides DvMF_2295 and DvMF_2296 are separated by 256 nucleotides DvMF_2296 and DvMF_2297 are separated by 39 nucleotides
DvMF_2293: DvMF_2293 - PP-loop domain protein (RefSeq), at 2,881,458 to 2,882,204
_2293
DvMF_2294: DvMF_2294 - DNA-directed RNA polymerase, omega subunit (RefSeq), at 2,882,854 to 2,883,081
_2294
DvMF_2295: DvMF_2295 - chaperone protein DnaJ (RefSeq), at 2,883,088 to 2,884,212
_2295
DvMF_2296: DvMF_2296 - hypothetical protein (RefSeq), at 2,884,469 to 2,885,122
_2296
DvMF_2297: DvMF_2297 - hypothetical protein (RefSeq), at 2,885,162 to 2,885,677
_2297
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-2
-1
0
1 at 2882.171 kb on + strand at 2882.179 kb on + strand at 2882.464 kb on - strand at 2882.768 kb on + strand at 2883.112 kb on - strand at 2883.647 kb on + strand, within DvMF_2295 at 2883.855 kb on + strand, within DvMF_2295 at 2884.034 kb on + strand, within DvMF_2295 at 2884.088 kb on + strand, within DvMF_2295 at 2884.554 kb on + strand, within DvMF_2296 at 2884.640 kb on - strand, within DvMF_2296 at 2884.668 kb on - strand, within DvMF_2296 at 2884.702 kb on + strand, within DvMF_2296 at 2884.735 kb on + strand, within DvMF_2296 at 2884.951 kb on - strand, within DvMF_2296 at 2885.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 2,882,171 + -0.3 2,882,179 + -1.2 2,882,464 - -0.7 2,882,768 + -0.4 2,883,112 - -0.7 2,883,647 + DvMF_2295 0.50 -1.4 2,883,855 + DvMF_2295 0.68 -0.6 2,884,034 + DvMF_2295 0.84 -1.9 2,884,088 + DvMF_2295 0.89 -0.6 2,884,554 + DvMF_2296 0.13 -0.2 2,884,640 - DvMF_2296 0.26 +0.4 2,884,668 - DvMF_2296 0.30 -0.3 2,884,702 + DvMF_2296 0.36 +0.7 2,884,735 + DvMF_2296 0.41 -0.2 2,884,951 - DvMF_2296 0.74 -0.4 2,885,067 + -0.1
Or see this region's nucleotide sequence