Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2219 and DvMF_2220 are separated by 85 nucleotides DvMF_2220 and DvMF_2221 are separated by 178 nucleotides DvMF_2221 and DvMF_2222 overlap by 4 nucleotides
DvMF_2219: DvMF_2219 - nuclease (SNase domain protein) (RefSeq), at 2,779,206 to 2,779,823
_2219
DvMF_2220: DvMF_2220 - lipolytic protein G-D-S-L family (RefSeq), at 2,779,909 to 2,780,553
_2220
DvMF_2221: DvMF_2221 - ABC transporter related (RefSeq), at 2,780,732 to 2,781,490
_2221
DvMF_2222: DvMF_2222 - protein of unknown function DUF214 (RefSeq), at 2,781,487 to 2,784,294
_2222
Position (kb)
2779
2780
2781 Strain fitness (log2 ratio)
-1
0
1
2 at 2778.965 kb on - strand at 2779.110 kb on + strand at 2779.202 kb on - strand at 2779.315 kb on + strand, within DvMF_2219 at 2779.687 kb on - strand, within DvMF_2219 at 2780.026 kb on + strand, within DvMF_2220 at 2780.034 kb on - strand, within DvMF_2220 at 2780.282 kb on - strand, within DvMF_2220 at 2780.620 kb on - strand at 2780.803 kb on + strand at 2780.886 kb on + strand, within DvMF_2221 at 2781.040 kb on + strand, within DvMF_2221 at 2781.050 kb on - strand, within DvMF_2221 at 2781.060 kb on + strand, within DvMF_2221
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 2,778,965 - +1.2 2,779,110 + -0.2 2,779,202 - -0.3 2,779,315 + DvMF_2219 0.18 -0.5 2,779,687 - DvMF_2219 0.78 -0.2 2,780,026 + DvMF_2220 0.18 -0.4 2,780,034 - DvMF_2220 0.19 -0.6 2,780,282 - DvMF_2220 0.58 +1.8 2,780,620 - -0.1 2,780,803 + -0.3 2,780,886 + DvMF_2221 0.20 -0.6 2,781,040 + DvMF_2221 0.41 -0.5 2,781,050 - DvMF_2221 0.42 +0.6 2,781,060 + DvMF_2221 0.43 +0.0
Or see this region's nucleotide sequence