Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2143 and DvMF_2144 are separated by 94 nucleotides DvMF_2144 and DvMF_2145 overlap by 4 nucleotides DvMF_2145 and DvMF_2146 are separated by 325 nucleotides
DvMF_2143: DvMF_2143 - dihydroorotate dehydrogenase family protein (RefSeq), at 2,669,027 to 2,669,953
_2143
DvMF_2144: DvMF_2144 - GCN5-related N-acetyltransferase (RefSeq), at 2,670,048 to 2,670,587
_2144
DvMF_2145: DvMF_2145 - Protein of unknown function DUF1778 (RefSeq), at 2,670,584 to 2,670,856
_2145
DvMF_2146: DvMF_2146 - delta-1-pyrroline-5-carboxylate dehydrogenase (RefSeq), at 2,671,182 to 2,674,223
_2146
Position (kb)
2670
2671 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2669.657 kb on - strand, within DvMF_2143 at 2669.665 kb on + strand, within DvMF_2143 at 2669.929 kb on + strand at 2670.045 kb on + strand at 2670.045 kb on + strand at 2670.053 kb on - strand at 2670.053 kb on - strand at 2670.281 kb on + strand, within DvMF_2144 at 2670.416 kb on - strand, within DvMF_2144 at 2670.755 kb on + strand, within DvMF_2145 at 2671.187 kb on - strand at 2671.370 kb on + strand at 2671.456 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 2,669,657 - DvMF_2143 0.68 -4.1 2,669,665 + DvMF_2143 0.69 -0.9 2,669,929 + -0.2 2,670,045 + -0.6 2,670,045 + -0.4 2,670,053 - -0.7 2,670,053 - -0.7 2,670,281 + DvMF_2144 0.43 -0.9 2,670,416 - DvMF_2144 0.68 -0.2 2,670,755 + DvMF_2145 0.63 +0.2 2,671,187 - +0.3 2,671,370 + -0.7 2,671,456 - -0.5
Or see this region's nucleotide sequence