Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1762 and DvMF_R0049 are separated by 603 nucleotides DvMF_R0049 and DvMF_1763 are separated by 86 nucleotides DvMF_1763 and DvMF_1764 are separated by 249 nucleotides
DvMF_1762: DvMF_1762 - riboflavin biosynthesis protein RibF (RefSeq), at 2,157,797 to 2,158,741
_1762
DvMF_R0049: DvMF_R0049 - tRNA-Leu (RefSeq), at 2,159,345 to 2,159,431
_R0049
DvMF_1763: DvMF_1763 - sigma54 specific transcriptional regulator, Fis family (RefSeq), at 2,159,518 to 2,160,684
_1763
DvMF_1764: DvMF_1764 - protein of unknown function DUF81 (RefSeq), at 2,160,934 to 2,161,911
_1764
Position (kb)
2159
2160
2161 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2158.862 kb on - strand at 2159.045 kb on + strand at 2159.112 kb on + strand at 2159.136 kb on + strand at 2159.139 kb on - strand at 2159.139 kb on - strand at 2159.181 kb on + strand at 2159.188 kb on + strand at 2159.223 kb on + strand at 2159.416 kb on - strand, within DvMF_R0049 at 2160.133 kb on - strand, within DvMF_1763 at 2160.331 kb on + strand, within DvMF_1763 at 2160.331 kb on + strand, within DvMF_1763 at 2160.339 kb on - strand, within DvMF_1763 at 2160.355 kb on + strand, within DvMF_1763 at 2160.355 kb on + strand, within DvMF_1763 at 2160.405 kb on - strand, within DvMF_1763
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 2,158,862 - -0.3 2,159,045 + -0.4 2,159,112 + -0.4 2,159,136 + -0.4 2,159,139 - -0.4 2,159,139 - -0.4 2,159,181 + -1.0 2,159,188 + +2.1 2,159,223 + +0.6 2,159,416 - DvMF_R0049 0.82 -0.2 2,160,133 - DvMF_1763 0.53 -0.4 2,160,331 + DvMF_1763 0.70 -1.3 2,160,331 + DvMF_1763 0.70 -1.3 2,160,339 - DvMF_1763 0.70 -2.4 2,160,355 + DvMF_1763 0.72 -1.7 2,160,355 + DvMF_1763 0.72 -1.4 2,160,405 - DvMF_1763 0.76 -1.3
Or see this region's nucleotide sequence