Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_1172 and DvMF_1173 are separated by 148 nucleotides DvMF_1173 and DvMF_1174 are separated by 340 nucleotides DvMF_1174 and DvMF_1175 overlap by 4 nucleotides
DvMF_1172: DvMF_1172 - Rhodanese domain protein (RefSeq), at 1,449,223 to 1,449,744
_1172
DvMF_1173: DvMF_1173 - hypothetical protein (RefSeq), at 1,449,893 to 1,450,369
_1173
DvMF_1174: DvMF_1174 - cobalamin (vitamin B12) biosynthesis CbiD protein (RefSeq), at 1,450,710 to 1,452,059
_1174
DvMF_1175: DvMF_1175 - precorrin-6y C5,15-methyltransferase (decarboxylating), CbiE subunit (RefSeq), at 1,452,056 to 1,453,480
_1175
Position (kb)
1450
1451
1452
1453 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1449.952 kb on + strand, within DvMF_1173 at 1449.960 kb on - strand, within DvMF_1173 at 1450.004 kb on + strand, within DvMF_1173 at 1450.200 kb on - strand, within DvMF_1173 at 1450.872 kb on - strand, within DvMF_1174 at 1451.043 kb on - strand, within DvMF_1174 at 1451.337 kb on - strand, within DvMF_1174 at 1451.614 kb on + strand, within DvMF_1174 at 1451.646 kb on - strand, within DvMF_1174 at 1451.717 kb on - strand, within DvMF_1174 at 1451.907 kb on - strand, within DvMF_1174 at 1453.053 kb on + strand, within DvMF_1175
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 1,449,952 + DvMF_1173 0.12 -0.2 1,449,960 - DvMF_1173 0.14 -0.8 1,450,004 + DvMF_1173 0.23 -0.4 1,450,200 - DvMF_1173 0.64 -1.0 1,450,872 - DvMF_1174 0.12 -2.4 1,451,043 - DvMF_1174 0.25 -1.1 1,451,337 - DvMF_1174 0.46 -0.9 1,451,614 + DvMF_1174 0.67 -0.5 1,451,646 - DvMF_1174 0.69 -1.8 1,451,717 - DvMF_1174 0.75 -0.3 1,451,907 - DvMF_1174 0.89 -1.2 1,453,053 + DvMF_1175 0.70 -2.8
Or see this region's nucleotide sequence