Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0322 and DvMF_0323 overlap by 4 nucleotides DvMF_0323 and DvMF_0324 overlap by 4 nucleotides DvMF_0324 and DvMF_0325 overlap by 13 nucleotides
DvMF_0322: DvMF_0322 - heterodisulfide reductase, C subunit (RefSeq), at 375,516 to 376,076
_0322
DvMF_0323: DvMF_0323 - CoB--CoM heterodisulfide reductase (RefSeq), at 376,073 to 377,053
_0323
DvMF_0324: DvMF_0324 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 377,050 to 379,008
_0324
DvMF_0325: DvMF_0325 - methyl-viologen-reducing hydrogenase delta subunit (RefSeq), at 378,996 to 380,501
_0325
Position (kb) 377
378
379
380 Strain fitness (log2 ratio) -1
0
1
2 at 376.676 kb on - strand, within DvMF_0323 at 376.828 kb on - strand, within DvMF_0323 at 376.981 kb on + strand at 377.019 kb on - strand at 377.118 kb on + strand at 377.216 kb on + strand at 377.502 kb on + strand, within DvMF_0324 at 377.502 kb on + strand, within DvMF_0324 at 377.520 kb on - strand, within DvMF_0324 at 378.049 kb on - strand, within DvMF_0324 at 378.049 kb on - strand, within DvMF_0324 at 378.100 kb on - strand, within DvMF_0324 at 378.100 kb on - strand, within DvMF_0324 at 378.387 kb on - strand, within DvMF_0324 at 378.721 kb on - strand at 378.984 kb on + strand at 379.359 kb on + strand, within DvMF_0325 at 379.747 kb on + strand, within DvMF_0325 at 379.777 kb on + strand, within DvMF_0325
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 376,676 - DvMF_0323 0.61 -0.3 376,828 - DvMF_0323 0.77 -0.2 376,981 + -0.6 377,019 - -0.7 377,118 + -0.7 377,216 + +0.0 377,502 + DvMF_0324 0.23 -0.3 377,502 + DvMF_0324 0.23 -0.4 377,520 - DvMF_0324 0.24 +0.2 378,049 - DvMF_0324 0.51 +0.2 378,049 - DvMF_0324 0.51 -0.1 378,100 - DvMF_0324 0.54 -0.2 378,100 - DvMF_0324 0.54 -0.3 378,387 - DvMF_0324 0.68 +2.1 378,721 - +0.8 378,984 + -0.0 379,359 + DvMF_0325 0.24 -0.2 379,747 + DvMF_0325 0.50 +0.2 379,777 + DvMF_0325 0.52 -0.8
Or see this region's nucleotide sequence