Experiment: BG11 with nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clpX and Synpcc7942_2527 overlap by 4 nucleotides Synpcc7942_2527 and Synpcc7942_2528 are separated by 23 nucleotides
Synpcc7942_2526: clpX - ATP-dependent protease ATP-binding subunit, at 2,607,529 to 2,608,878
clpX
Synpcc7942_2527: Synpcc7942_2527 - esterase-like, at 2,608,875 to 2,609,717
_2527
Synpcc7942_2528: Synpcc7942_2528 - hypothetical protein, at 2,609,741 to 2,610,427
_2528
Position (kb)
2608
2609
2610 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2607.952 kb on + strand, within clpX at 2608.231 kb on - strand, within clpX at 2608.267 kb on - strand, within clpX at 2608.418 kb on + strand, within clpX at 2608.488 kb on + strand, within clpX at 2608.540 kb on + strand, within clpX at 2608.610 kb on - strand, within clpX at 2608.637 kb on + strand, within clpX at 2608.829 kb on - strand at 2608.893 kb on + strand at 2608.893 kb on + strand at 2608.900 kb on + strand at 2608.901 kb on - strand at 2608.901 kb on - strand at 2608.901 kb on - strand at 2608.951 kb on + strand at 2609.018 kb on - strand, within Synpcc7942_2527 at 2609.028 kb on + strand, within Synpcc7942_2527 at 2609.036 kb on - strand, within Synpcc7942_2527 at 2609.058 kb on - strand, within Synpcc7942_2527 at 2609.079 kb on + strand, within Synpcc7942_2527 at 2609.084 kb on + strand, within Synpcc7942_2527 at 2609.087 kb on - strand, within Synpcc7942_2527 at 2609.092 kb on - strand, within Synpcc7942_2527 at 2609.092 kb on - strand, within Synpcc7942_2527 at 2609.168 kb on + strand, within Synpcc7942_2527 at 2609.176 kb on - strand, within Synpcc7942_2527 at 2609.176 kb on - strand, within Synpcc7942_2527 at 2609.177 kb on - strand, within Synpcc7942_2527 at 2609.183 kb on - strand, within Synpcc7942_2527 at 2609.198 kb on + strand, within Synpcc7942_2527 at 2609.212 kb on - strand, within Synpcc7942_2527 at 2609.234 kb on - strand, within Synpcc7942_2527 at 2609.265 kb on + strand, within Synpcc7942_2527 at 2609.273 kb on - strand, within Synpcc7942_2527 at 2609.299 kb on + strand, within Synpcc7942_2527 at 2609.397 kb on - strand, within Synpcc7942_2527 at 2609.398 kb on + strand, within Synpcc7942_2527 at 2609.453 kb on - strand, within Synpcc7942_2527 at 2609.457 kb on + strand, within Synpcc7942_2527 at 2609.482 kb on + strand, within Synpcc7942_2527 at 2609.501 kb on + strand, within Synpcc7942_2527 at 2609.501 kb on + strand, within Synpcc7942_2527 at 2609.509 kb on - strand, within Synpcc7942_2527 at 2609.527 kb on - strand, within Synpcc7942_2527 at 2609.537 kb on - strand, within Synpcc7942_2527 at 2609.553 kb on - strand, within Synpcc7942_2527 at 2609.567 kb on + strand, within Synpcc7942_2527 at 2609.572 kb on + strand, within Synpcc7942_2527 at 2609.589 kb on + strand, within Synpcc7942_2527 at 2609.590 kb on + strand, within Synpcc7942_2527 at 2609.590 kb on - strand, within Synpcc7942_2527 at 2609.628 kb on + strand, within Synpcc7942_2527 at 2609.628 kb on + strand, within Synpcc7942_2527 at 2609.712 kb on - strand at 2609.731 kb on + strand at 2609.736 kb on + strand at 2609.747 kb on - strand at 2609.781 kb on - strand at 2609.783 kb on + strand at 2609.783 kb on + strand at 2609.783 kb on + strand at 2609.785 kb on + strand at 2609.791 kb on - strand at 2609.791 kb on - strand at 2609.823 kb on + strand, within Synpcc7942_2528 at 2609.830 kb on - strand, within Synpcc7942_2528 at 2609.830 kb on - strand, within Synpcc7942_2528 at 2609.889 kb on + strand, within Synpcc7942_2528 at 2609.910 kb on - strand, within Synpcc7942_2528 at 2609.912 kb on - strand, within Synpcc7942_2528 at 2609.968 kb on + strand, within Synpcc7942_2528 at 2609.971 kb on - strand, within Synpcc7942_2528 at 2609.981 kb on + strand, within Synpcc7942_2528 at 2610.011 kb on - strand, within Synpcc7942_2528 at 2610.017 kb on + strand, within Synpcc7942_2528 at 2610.036 kb on - strand, within Synpcc7942_2528 at 2610.091 kb on - strand, within Synpcc7942_2528 at 2610.091 kb on - strand, within Synpcc7942_2528 at 2610.118 kb on - strand, within Synpcc7942_2528 at 2610.129 kb on + strand, within Synpcc7942_2528 at 2610.136 kb on - strand, within Synpcc7942_2528 at 2610.156 kb on - strand, within Synpcc7942_2528 at 2610.158 kb on + strand, within Synpcc7942_2528 at 2610.185 kb on + strand, within Synpcc7942_2528 at 2610.187 kb on - strand, within Synpcc7942_2528 at 2610.262 kb on - strand, within Synpcc7942_2528 at 2610.323 kb on + strand, within Synpcc7942_2528 at 2610.336 kb on - strand, within Synpcc7942_2528 at 2610.386 kb on - strand at 2610.406 kb on - strand at 2610.423 kb on - strand at 2610.457 kb on - strand at 2610.475 kb on + strand at 2610.489 kb on + strand at 2610.496 kb on + strand at 2610.508 kb on + strand at 2610.508 kb on + strand at 2610.595 kb on - strand at 2610.648 kb on - strand at 2610.651 kb on + strand at 2610.659 kb on + strand at 2610.701 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrite 20 mM remove 2,607,952 + clpX Synpcc7942_2526 0.31 -2.5 2,608,231 - clpX Synpcc7942_2526 0.52 -1.9 2,608,267 - clpX Synpcc7942_2526 0.55 -1.7 2,608,418 + clpX Synpcc7942_2526 0.66 +0.8 2,608,488 + clpX Synpcc7942_2526 0.71 -2.5 2,608,540 + clpX Synpcc7942_2526 0.75 -2.2 2,608,610 - clpX Synpcc7942_2526 0.80 -1.7 2,608,637 + clpX Synpcc7942_2526 0.82 -3.7 2,608,829 - -0.8 2,608,893 + -0.5 2,608,893 + +0.8 2,608,900 + +0.9 2,608,901 - +0.3 2,608,901 - +1.0 2,608,901 - +1.2 2,608,951 + +1.7 2,609,018 - Synpcc7942_2527 0.17 -1.4 2,609,028 + Synpcc7942_2527 0.18 +0.1 2,609,036 - Synpcc7942_2527 0.19 -0.4 2,609,058 - Synpcc7942_2527 0.22 +0.4 2,609,079 + Synpcc7942_2527 0.24 -2.4 2,609,084 + Synpcc7942_2527 0.25 +1.0 2,609,087 - Synpcc7942_2527 0.25 -1.0 2,609,092 - Synpcc7942_2527 0.26 +0.7 2,609,092 - Synpcc7942_2527 0.26 -0.4 2,609,168 + Synpcc7942_2527 0.35 -1.0 2,609,176 - Synpcc7942_2527 0.36 -0.2 2,609,176 - Synpcc7942_2527 0.36 +0.2 2,609,177 - Synpcc7942_2527 0.36 +0.6 2,609,183 - Synpcc7942_2527 0.37 -0.4 2,609,198 + Synpcc7942_2527 0.38 +0.3 2,609,212 - Synpcc7942_2527 0.40 -0.1 2,609,234 - Synpcc7942_2527 0.43 -0.5 2,609,265 + Synpcc7942_2527 0.46 +0.1 2,609,273 - Synpcc7942_2527 0.47 +0.2 2,609,299 + Synpcc7942_2527 0.50 +0.1 2,609,397 - Synpcc7942_2527 0.62 +0.7 2,609,398 + Synpcc7942_2527 0.62 +0.4 2,609,453 - Synpcc7942_2527 0.69 -1.2 2,609,457 + Synpcc7942_2527 0.69 +0.5 2,609,482 + Synpcc7942_2527 0.72 -0.2 2,609,501 + Synpcc7942_2527 0.74 -0.2 2,609,501 + Synpcc7942_2527 0.74 +1.0 2,609,509 - Synpcc7942_2527 0.75 -1.4 2,609,527 - Synpcc7942_2527 0.77 -2.2 2,609,537 - Synpcc7942_2527 0.79 +0.2 2,609,553 - Synpcc7942_2527 0.80 -0.7 2,609,567 + Synpcc7942_2527 0.82 +0.4 2,609,572 + Synpcc7942_2527 0.83 +1.1 2,609,589 + Synpcc7942_2527 0.85 +0.8 2,609,590 + Synpcc7942_2527 0.85 +1.3 2,609,590 - Synpcc7942_2527 0.85 +1.7 2,609,628 + Synpcc7942_2527 0.89 -0.9 2,609,628 + Synpcc7942_2527 0.89 -0.4 2,609,712 - +0.2 2,609,731 + +1.1 2,609,736 + -0.9 2,609,747 - -1.2 2,609,781 - -0.9 2,609,783 + +0.6 2,609,783 + -1.0 2,609,783 + -0.9 2,609,785 + +1.3 2,609,791 - +1.5 2,609,791 - +0.2 2,609,823 + Synpcc7942_2528 0.12 +0.7 2,609,830 - Synpcc7942_2528 0.13 -1.5 2,609,830 - Synpcc7942_2528 0.13 +0.4 2,609,889 + Synpcc7942_2528 0.22 -0.7 2,609,910 - Synpcc7942_2528 0.25 +1.0 2,609,912 - Synpcc7942_2528 0.25 -0.3 2,609,968 + Synpcc7942_2528 0.33 +1.2 2,609,971 - Synpcc7942_2528 0.33 -0.4 2,609,981 + Synpcc7942_2528 0.35 +0.7 2,610,011 - Synpcc7942_2528 0.39 +1.0 2,610,017 + Synpcc7942_2528 0.40 -0.6 2,610,036 - Synpcc7942_2528 0.43 +0.1 2,610,091 - Synpcc7942_2528 0.51 -0.8 2,610,091 - Synpcc7942_2528 0.51 -1.7 2,610,118 - Synpcc7942_2528 0.55 -1.2 2,610,129 + Synpcc7942_2528 0.56 +0.1 2,610,136 - Synpcc7942_2528 0.57 +0.6 2,610,156 - Synpcc7942_2528 0.60 -0.1 2,610,158 + Synpcc7942_2528 0.61 -0.4 2,610,185 + Synpcc7942_2528 0.65 -0.6 2,610,187 - Synpcc7942_2528 0.65 +0.2 2,610,262 - Synpcc7942_2528 0.76 -0.5 2,610,323 + Synpcc7942_2528 0.85 +0.1 2,610,336 - Synpcc7942_2528 0.87 +0.4 2,610,386 - -0.3 2,610,406 - -0.8 2,610,423 - -0.7 2,610,457 - -1.2 2,610,475 + +0.3 2,610,489 + -0.8 2,610,496 + -0.8 2,610,508 + -0.5 2,610,508 + -1.1 2,610,595 - -1.7 2,610,648 - +0.2 2,610,651 + -1.8 2,610,659 + +1.1 2,610,701 + +0.6
Or see this region's nucleotide sequence