Experiment: BG11 with nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livF and natA overlap by 8 nucleotides natA and livM overlap by 4 nucleotides livM and natD are separated by 3 nucleotides natD and livJ overlap by 1 nucleotides
Synpcc7942_2492: livF - ATPase, at 2,573,355 to 2,574,068
livF
Synpcc7942_2493: natA - ATPase, at 2,574,061 to 2,574,822
natA
Synpcc7942_2494: livM - putative branched-chain amino acid ABC transporter, permease protein, at 2,574,819 to 2,575,742
livM
Synpcc7942_2495: natD - integral membrane protein of the ABC-type Nat permease for neutral amino acids NatD, at 2,575,746 to 2,576,687
natD
Synpcc7942_2496: livJ - branched-chain amino-acid ABC transport system periplasmic binding protein, at 2,576,687 to 2,577,886
livJ
Position (kb)
2574
2575
2576 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2573.825 kb on - strand, within livF at 2573.828 kb on - strand, within livF at 2573.834 kb on - strand, within livF at 2573.868 kb on + strand, within livF at 2573.881 kb on - strand, within livF at 2573.883 kb on + strand, within livF at 2573.910 kb on - strand, within livF at 2573.942 kb on - strand, within livF at 2573.961 kb on - strand, within livF at 2574.004 kb on + strand at 2574.014 kb on + strand at 2574.022 kb on + strand at 2574.049 kb on - strand at 2574.066 kb on - strand at 2574.076 kb on - strand at 2574.080 kb on + strand at 2574.110 kb on - strand at 2574.145 kb on + strand, within natA at 2574.174 kb on - strand, within natA at 2574.193 kb on - strand, within natA at 2574.201 kb on - strand at 2574.208 kb on - strand, within natA at 2574.244 kb on - strand, within natA at 2574.290 kb on + strand, within natA at 2574.382 kb on - strand, within natA at 2574.400 kb on - strand, within natA at 2574.410 kb on - strand, within natA at 2574.421 kb on + strand, within natA at 2574.455 kb on - strand, within natA at 2574.456 kb on - strand, within natA at 2574.480 kb on + strand at 2574.523 kb on - strand, within natA at 2574.547 kb on - strand, within natA at 2574.566 kb on - strand, within natA at 2574.584 kb on + strand, within natA at 2574.587 kb on - strand, within natA at 2574.603 kb on + strand, within natA at 2574.607 kb on - strand, within natA at 2574.648 kb on - strand, within natA at 2574.663 kb on + strand, within natA at 2574.671 kb on - strand, within natA at 2574.671 kb on - strand, within natA at 2574.690 kb on + strand, within natA at 2574.695 kb on + strand, within natA at 2574.707 kb on + strand, within natA at 2574.713 kb on + strand, within natA at 2574.715 kb on - strand, within natA at 2574.723 kb on + strand, within natA at 2574.723 kb on + strand, within natA at 2574.723 kb on + strand, within natA at 2574.723 kb on + strand, within natA at 2574.764 kb on - strand at 2574.771 kb on + strand at 2574.790 kb on - strand at 2574.823 kb on - strand at 2574.839 kb on - strand at 2574.896 kb on - strand at 2574.937 kb on + strand, within livM at 2574.942 kb on + strand, within livM at 2574.950 kb on - strand, within livM at 2574.950 kb on - strand, within livM at 2574.953 kb on + strand, within livM at 2574.992 kb on - strand, within livM at 2574.997 kb on - strand, within livM at 2575.007 kb on + strand, within livM at 2575.007 kb on + strand, within livM at 2575.015 kb on - strand, within livM at 2575.017 kb on + strand, within livM at 2575.025 kb on - strand, within livM at 2575.025 kb on - strand, within livM at 2575.025 kb on - strand, within livM at 2575.025 kb on - strand, within livM at 2575.029 kb on + strand, within livM at 2575.035 kb on - strand, within livM at 2575.036 kb on + strand, within livM at 2575.036 kb on + strand, within livM at 2575.037 kb on - strand, within livM at 2575.048 kb on + strand, within livM at 2575.058 kb on + strand, within livM at 2575.065 kb on + strand, within livM at 2575.075 kb on - strand, within livM at 2575.082 kb on - strand, within livM at 2575.083 kb on + strand, within livM at 2575.085 kb on + strand, within livM at 2575.114 kb on - strand, within livM at 2575.119 kb on + strand, within livM at 2575.129 kb on + strand, within livM at 2575.142 kb on + strand, within livM at 2575.152 kb on + strand, within livM at 2575.152 kb on + strand, within livM at 2575.152 kb on + strand at 2575.160 kb on - strand, within livM at 2575.160 kb on - strand, within livM at 2575.164 kb on + strand, within livM at 2575.176 kb on + strand, within livM at 2575.189 kb on - strand, within livM at 2575.216 kb on - strand, within livM at 2575.218 kb on + strand, within livM at 2575.224 kb on + strand, within livM at 2575.226 kb on - strand at 2575.250 kb on + strand, within livM at 2575.250 kb on + strand, within livM at 2575.251 kb on + strand, within livM at 2575.258 kb on - strand, within livM at 2575.258 kb on - strand, within livM at 2575.259 kb on - strand, within livM at 2575.261 kb on + strand, within livM at 2575.350 kb on + strand, within livM at 2575.350 kb on + strand, within livM at 2575.350 kb on + strand, within livM at 2575.470 kb on + strand, within livM at 2575.470 kb on + strand, within livM at 2575.472 kb on + strand, within livM at 2575.480 kb on - strand, within livM at 2575.502 kb on - strand, within livM at 2575.502 kb on - strand, within livM at 2575.517 kb on + strand, within livM at 2575.517 kb on + strand, within livM at 2575.517 kb on + strand, within livM at 2575.544 kb on + strand, within livM at 2575.552 kb on - strand, within livM at 2575.552 kb on - strand, within livM at 2575.554 kb on + strand, within livM at 2575.556 kb on + strand, within livM at 2575.556 kb on + strand, within livM at 2575.656 kb on + strand at 2575.671 kb on - strand at 2575.676 kb on + strand at 2575.680 kb on - strand at 2575.682 kb on + strand at 2575.692 kb on - strand at 2575.716 kb on + strand at 2575.724 kb on - strand at 2575.732 kb on + strand at 2575.743 kb on + strand at 2575.743 kb on + strand at 2575.743 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.744 kb on + strand at 2575.749 kb on + strand at 2575.751 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.752 kb on - strand at 2575.767 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.776 kb on - strand at 2575.786 kb on - strand at 2575.787 kb on - strand at 2575.799 kb on - strand at 2575.799 kb on - strand at 2575.799 kb on - strand at 2575.800 kb on - strand at 2575.804 kb on + strand at 2575.812 kb on - strand at 2575.821 kb on + strand at 2575.822 kb on - strand at 2575.846 kb on - strand, within natD at 2575.884 kb on - strand, within natD at 2575.897 kb on + strand, within natD at 2575.923 kb on + strand, within natD at 2575.923 kb on + strand, within natD at 2575.932 kb on - strand, within natD at 2575.937 kb on - strand, within natD at 2575.946 kb on + strand, within natD at 2575.991 kb on + strand, within natD at 2576.000 kb on + strand, within natD at 2576.092 kb on - strand, within natD at 2576.113 kb on + strand, within natD at 2576.129 kb on - strand, within natD at 2576.148 kb on - strand, within natD at 2576.149 kb on + strand, within natD at 2576.161 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.196 kb on + strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.204 kb on - strand, within natD at 2576.214 kb on - strand, within natD at 2576.214 kb on - strand, within natD at 2576.219 kb on - strand, within natD at 2576.219 kb on - strand, within natD at 2576.222 kb on + strand, within natD at 2576.230 kb on - strand, within natD at 2576.243 kb on - strand, within natD at 2576.249 kb on - strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.253 kb on + strand, within natD at 2576.256 kb on - strand at 2576.258 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.261 kb on - strand, within natD at 2576.263 kb on + strand, within natD at 2576.270 kb on - strand at 2576.271 kb on - strand, within natD at 2576.282 kb on - strand, within natD at 2576.282 kb on - strand, within natD at 2576.284 kb on + strand, within natD at 2576.357 kb on + strand, within natD at 2576.364 kb on - strand, within natD at 2576.364 kb on - strand, within natD at 2576.364 kb on - strand, within natD at 2576.392 kb on + strand, within natD at 2576.413 kb on + strand, within natD at 2576.413 kb on + strand, within natD at 2576.464 kb on - strand, within natD at 2576.476 kb on + strand, within natD at 2576.479 kb on - strand, within natD at 2576.484 kb on - strand, within natD at 2576.484 kb on - strand, within natD at 2576.486 kb on + strand, within natD at 2576.536 kb on + strand, within natD at 2576.544 kb on - strand, within natD at 2576.592 kb on + strand, within natD at 2576.613 kb on + strand at 2576.615 kb on - strand at 2576.621 kb on - strand at 2576.622 kb on - strand at 2576.641 kb on - strand at 2576.641 kb on - strand at 2576.641 kb on - strand at 2576.657 kb on + strand at 2576.657 kb on + strand at 2576.667 kb on - strand at 2576.698 kb on + strand at 2576.698 kb on + strand at 2576.707 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrite 20 mM remove 2,573,825 - livF Synpcc7942_2492 0.66 +0.0 2,573,828 - livF Synpcc7942_2492 0.66 -0.6 2,573,834 - livF Synpcc7942_2492 0.67 -1.2 2,573,868 + livF Synpcc7942_2492 0.72 -1.1 2,573,881 - livF Synpcc7942_2492 0.74 +0.0 2,573,883 + livF Synpcc7942_2492 0.74 -0.1 2,573,910 - livF Synpcc7942_2492 0.78 -2.9 2,573,942 - livF Synpcc7942_2492 0.82 -0.0 2,573,961 - livF Synpcc7942_2492 0.85 -0.9 2,574,004 + +0.5 2,574,014 + +0.3 2,574,022 + -0.8 2,574,049 - +0.8 2,574,066 - -0.1 2,574,076 - +0.2 2,574,080 + +0.0 2,574,110 - +0.2 2,574,145 + natA Synpcc7942_2493 0.11 -1.0 2,574,174 - natA Synpcc7942_2493 0.15 -0.2 2,574,193 - natA Synpcc7942_2493 0.17 -1.2 2,574,201 - -0.7 2,574,208 - natA Synpcc7942_2493 0.19 -1.6 2,574,244 - natA Synpcc7942_2493 0.24 +0.2 2,574,290 + natA Synpcc7942_2493 0.30 -0.1 2,574,382 - natA Synpcc7942_2493 0.42 -0.4 2,574,400 - natA Synpcc7942_2493 0.44 -1.9 2,574,410 - natA Synpcc7942_2493 0.46 -1.3 2,574,421 + natA Synpcc7942_2493 0.47 -1.0 2,574,455 - natA Synpcc7942_2493 0.52 -1.8 2,574,456 - natA Synpcc7942_2493 0.52 -0.4 2,574,480 + +1.1 2,574,523 - natA Synpcc7942_2493 0.61 +0.8 2,574,547 - natA Synpcc7942_2493 0.64 -0.0 2,574,566 - natA Synpcc7942_2493 0.66 +0.0 2,574,584 + natA Synpcc7942_2493 0.69 -0.2 2,574,587 - natA Synpcc7942_2493 0.69 +0.3 2,574,603 + natA Synpcc7942_2493 0.71 -0.5 2,574,607 - natA Synpcc7942_2493 0.72 -0.7 2,574,648 - natA Synpcc7942_2493 0.77 +0.3 2,574,663 + natA Synpcc7942_2493 0.79 -2.2 2,574,671 - natA Synpcc7942_2493 0.80 -0.8 2,574,671 - natA Synpcc7942_2493 0.80 +0.9 2,574,690 + natA Synpcc7942_2493 0.83 -1.7 2,574,695 + natA Synpcc7942_2493 0.83 -1.0 2,574,707 + natA Synpcc7942_2493 0.85 -0.3 2,574,713 + natA Synpcc7942_2493 0.86 -1.7 2,574,715 - natA Synpcc7942_2493 0.86 +0.2 2,574,723 + natA Synpcc7942_2493 0.87 +0.5 2,574,723 + natA Synpcc7942_2493 0.87 -1.4 2,574,723 + natA Synpcc7942_2493 0.87 -1.2 2,574,723 + natA Synpcc7942_2493 0.87 +0.3 2,574,764 - -1.7 2,574,771 + +0.3 2,574,790 - -1.2 2,574,823 - -1.7 2,574,839 - -0.2 2,574,896 - +0.1 2,574,937 + livM Synpcc7942_2494 0.13 -0.5 2,574,942 + livM Synpcc7942_2494 0.13 -1.9 2,574,950 - livM Synpcc7942_2494 0.14 +0.9 2,574,950 - livM Synpcc7942_2494 0.14 -0.7 2,574,953 + livM Synpcc7942_2494 0.15 +0.8 2,574,992 - livM Synpcc7942_2494 0.19 +0.0 2,574,997 - livM Synpcc7942_2494 0.19 +0.9 2,575,007 + livM Synpcc7942_2494 0.20 +0.2 2,575,007 + livM Synpcc7942_2494 0.20 -0.2 2,575,015 - livM Synpcc7942_2494 0.21 -0.2 2,575,017 + livM Synpcc7942_2494 0.21 -1.8 2,575,025 - livM Synpcc7942_2494 0.22 +0.5 2,575,025 - livM Synpcc7942_2494 0.22 -0.8 2,575,025 - livM Synpcc7942_2494 0.22 -1.7 2,575,025 - livM Synpcc7942_2494 0.22 -1.7 2,575,029 + livM Synpcc7942_2494 0.23 -1.4 2,575,035 - livM Synpcc7942_2494 0.23 -0.3 2,575,036 + livM Synpcc7942_2494 0.23 -0.5 2,575,036 + livM Synpcc7942_2494 0.23 +0.0 2,575,037 - livM Synpcc7942_2494 0.24 -0.5 2,575,048 + livM Synpcc7942_2494 0.25 +0.1 2,575,058 + livM Synpcc7942_2494 0.26 +0.9 2,575,065 + livM Synpcc7942_2494 0.27 +0.5 2,575,075 - livM Synpcc7942_2494 0.28 +0.0 2,575,082 - livM Synpcc7942_2494 0.28 -0.3 2,575,083 + livM Synpcc7942_2494 0.29 -1.3 2,575,085 + livM Synpcc7942_2494 0.29 -0.6 2,575,114 - livM Synpcc7942_2494 0.32 +0.3 2,575,119 + livM Synpcc7942_2494 0.32 -0.0 2,575,129 + livM Synpcc7942_2494 0.34 -0.3 2,575,142 + livM Synpcc7942_2494 0.35 -0.8 2,575,152 + livM Synpcc7942_2494 0.36 +0.1 2,575,152 + livM Synpcc7942_2494 0.36 +0.0 2,575,152 + +0.3 2,575,160 - livM Synpcc7942_2494 0.37 -2.2 2,575,160 - livM Synpcc7942_2494 0.37 +0.6 2,575,164 + livM Synpcc7942_2494 0.37 -0.8 2,575,176 + livM Synpcc7942_2494 0.39 -0.5 2,575,189 - livM Synpcc7942_2494 0.40 -1.2 2,575,216 - livM Synpcc7942_2494 0.43 +0.5 2,575,218 + livM Synpcc7942_2494 0.43 -1.2 2,575,224 + livM Synpcc7942_2494 0.44 -0.6 2,575,226 - +2.1 2,575,250 + livM Synpcc7942_2494 0.47 +0.4 2,575,250 + livM Synpcc7942_2494 0.47 -0.7 2,575,251 + livM Synpcc7942_2494 0.47 -0.9 2,575,258 - livM Synpcc7942_2494 0.48 +0.9 2,575,258 - livM Synpcc7942_2494 0.48 +0.0 2,575,259 - livM Synpcc7942_2494 0.48 +0.3 2,575,261 + livM Synpcc7942_2494 0.48 -1.9 2,575,350 + livM Synpcc7942_2494 0.57 +0.1 2,575,350 + livM Synpcc7942_2494 0.57 -1.0 2,575,350 + livM Synpcc7942_2494 0.57 +0.4 2,575,470 + livM Synpcc7942_2494 0.70 +0.7 2,575,470 + livM Synpcc7942_2494 0.70 -0.6 2,575,472 + livM Synpcc7942_2494 0.71 -0.3 2,575,480 - livM Synpcc7942_2494 0.72 -0.8 2,575,502 - livM Synpcc7942_2494 0.74 -0.7 2,575,502 - livM Synpcc7942_2494 0.74 +0.8 2,575,517 + livM Synpcc7942_2494 0.76 +0.2 2,575,517 + livM Synpcc7942_2494 0.76 -0.3 2,575,517 + livM Synpcc7942_2494 0.76 -0.7 2,575,544 + livM Synpcc7942_2494 0.78 -1.2 2,575,552 - livM Synpcc7942_2494 0.79 -0.4 2,575,552 - livM Synpcc7942_2494 0.79 -0.2 2,575,554 + livM Synpcc7942_2494 0.80 +0.6 2,575,556 + livM Synpcc7942_2494 0.80 -0.6 2,575,556 + livM Synpcc7942_2494 0.80 -0.7 2,575,656 + -0.5 2,575,671 - -1.1 2,575,676 + -2.7 2,575,680 - -1.0 2,575,682 + -0.6 2,575,692 - -1.1 2,575,716 + -1.6 2,575,724 - +0.6 2,575,732 + -0.8 2,575,743 + +0.0 2,575,743 + -1.1 2,575,743 + +0.5 2,575,744 + +0.2 2,575,744 + +0.5 2,575,744 + +0.1 2,575,744 + -1.4 2,575,749 + +0.6 2,575,751 - +0.9 2,575,752 - +0.6 2,575,752 - +0.5 2,575,752 - -0.7 2,575,752 - -1.2 2,575,767 - -0.7 2,575,776 - -0.2 2,575,776 - -0.2 2,575,776 - +0.1 2,575,776 - -0.2 2,575,786 - -0.2 2,575,787 - -0.1 2,575,799 - +0.0 2,575,799 - +0.1 2,575,799 - -0.7 2,575,800 - +0.8 2,575,804 + +0.9 2,575,812 - +0.0 2,575,821 + -0.4 2,575,822 - -2.7 2,575,846 - natD Synpcc7942_2495 0.11 +0.2 2,575,884 - natD Synpcc7942_2495 0.15 -0.4 2,575,897 + natD Synpcc7942_2495 0.16 -1.3 2,575,923 + natD Synpcc7942_2495 0.19 -1.2 2,575,923 + natD Synpcc7942_2495 0.19 +0.1 2,575,932 - natD Synpcc7942_2495 0.20 -0.7 2,575,937 - natD Synpcc7942_2495 0.20 -1.9 2,575,946 + natD Synpcc7942_2495 0.21 -1.6 2,575,991 + natD Synpcc7942_2495 0.26 -1.4 2,576,000 + natD Synpcc7942_2495 0.27 -1.5 2,576,092 - natD Synpcc7942_2495 0.37 +0.6 2,576,113 + natD Synpcc7942_2495 0.39 -0.1 2,576,129 - natD Synpcc7942_2495 0.41 -0.9 2,576,148 - natD Synpcc7942_2495 0.43 -1.2 2,576,149 + natD Synpcc7942_2495 0.43 +0.5 2,576,161 + natD Synpcc7942_2495 0.44 -0.4 2,576,196 + natD Synpcc7942_2495 0.48 +0.8 2,576,196 + natD Synpcc7942_2495 0.48 -2.5 2,576,196 + natD Synpcc7942_2495 0.48 -0.7 2,576,204 - natD Synpcc7942_2495 0.49 -0.1 2,576,204 - natD Synpcc7942_2495 0.49 +0.3 2,576,204 - natD Synpcc7942_2495 0.49 -0.7 2,576,204 - natD Synpcc7942_2495 0.49 -1.0 2,576,214 - natD Synpcc7942_2495 0.50 -0.8 2,576,214 - natD Synpcc7942_2495 0.50 +0.6 2,576,219 - natD Synpcc7942_2495 0.50 +1.2 2,576,219 - natD Synpcc7942_2495 0.50 -0.5 2,576,222 + natD Synpcc7942_2495 0.51 -0.5 2,576,230 - natD Synpcc7942_2495 0.51 -0.5 2,576,243 - natD Synpcc7942_2495 0.53 +0.3 2,576,249 - natD Synpcc7942_2495 0.53 -0.8 2,576,253 + natD Synpcc7942_2495 0.54 +1.0 2,576,253 + natD Synpcc7942_2495 0.54 -2.0 2,576,253 + natD Synpcc7942_2495 0.54 -1.3 2,576,253 + natD Synpcc7942_2495 0.54 -0.7 2,576,256 - -1.1 2,576,258 - natD Synpcc7942_2495 0.54 -0.4 2,576,261 - natD Synpcc7942_2495 0.55 -1.2 2,576,261 - natD Synpcc7942_2495 0.55 -1.9 2,576,261 - natD Synpcc7942_2495 0.55 +1.2 2,576,263 + natD Synpcc7942_2495 0.55 -0.9 2,576,270 - +0.8 2,576,271 - natD Synpcc7942_2495 0.56 -0.1 2,576,282 - natD Synpcc7942_2495 0.57 -0.7 2,576,282 - natD Synpcc7942_2495 0.57 -0.5 2,576,284 + natD Synpcc7942_2495 0.57 -0.1 2,576,357 + natD Synpcc7942_2495 0.65 -0.2 2,576,364 - natD Synpcc7942_2495 0.66 -0.9 2,576,364 - natD Synpcc7942_2495 0.66 -1.1 2,576,364 - natD Synpcc7942_2495 0.66 -1.2 2,576,392 + natD Synpcc7942_2495 0.69 +0.9 2,576,413 + natD Synpcc7942_2495 0.71 +0.4 2,576,413 + natD Synpcc7942_2495 0.71 +0.7 2,576,464 - natD Synpcc7942_2495 0.76 -2.2 2,576,476 + natD Synpcc7942_2495 0.77 -0.3 2,576,479 - natD Synpcc7942_2495 0.78 -0.8 2,576,484 - natD Synpcc7942_2495 0.78 -2.0 2,576,484 - natD Synpcc7942_2495 0.78 +0.3 2,576,486 + natD Synpcc7942_2495 0.79 -0.2 2,576,536 + natD Synpcc7942_2495 0.84 -0.8 2,576,544 - natD Synpcc7942_2495 0.85 -0.2 2,576,592 + natD Synpcc7942_2495 0.90 -1.2 2,576,613 + -2.1 2,576,615 - +0.6 2,576,621 - +0.0 2,576,622 - +0.6 2,576,641 - -1.5 2,576,641 - -0.1 2,576,641 - -2.5 2,576,657 + -0.7 2,576,657 + -0.7 2,576,667 - -0.9 2,576,698 + -2.5 2,576,698 + -0.4 2,576,707 + +0.3
Or see this region's nucleotide sequence