Experiment: BG11 with nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ftsZ and thiD overlap by 1 nucleotides thiD and Synpcc7942_2380 overlap by 16 nucleotides Synpcc7942_2380 and ffs are separated by 123 nucleotides ffs and panB are separated by 79 nucleotides
Synpcc7942_2378: ftsZ - cell division protein FtsZ, at 2,445,387 to 2,446,568
ftsZ
Synpcc7942_2379: thiD - phosphomethylpyrimidine kinase, at 2,446,568 to 2,447,359
thiD
Synpcc7942_2380: Synpcc7942_2380 - hypothetical protein, at 2,447,344 to 2,447,772
_2380
Synpcc7942_R0047: ffs - SRP RNA, at 2,447,896 to 2,447,996
ffs
Synpcc7942_2381: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 2,448,076 to 2,448,882
panB
Position (kb)
2447
2448 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2446.571 kb on - strand at 2446.583 kb on + strand at 2446.591 kb on - strand at 2446.651 kb on + strand, within thiD at 2446.692 kb on - strand, within thiD at 2446.699 kb on + strand, within thiD at 2446.702 kb on - strand, within thiD at 2446.702 kb on - strand, within thiD at 2446.712 kb on - strand, within thiD at 2446.712 kb on - strand, within thiD at 2446.722 kb on - strand, within thiD at 2446.769 kb on - strand, within thiD at 2446.805 kb on + strand, within thiD at 2446.810 kb on + strand, within thiD at 2446.863 kb on - strand, within thiD at 2446.865 kb on + strand, within thiD at 2446.902 kb on + strand, within thiD at 2446.902 kb on + strand, within thiD at 2446.907 kb on + strand, within thiD at 2446.907 kb on - strand, within thiD at 2446.956 kb on - strand, within thiD at 2446.956 kb on - strand, within thiD at 2446.975 kb on - strand, within thiD at 2446.989 kb on + strand, within thiD at 2447.003 kb on + strand, within thiD at 2447.015 kb on + strand, within thiD at 2447.035 kb on + strand, within thiD at 2447.050 kb on + strand, within thiD at 2447.050 kb on + strand, within thiD at 2447.054 kb on + strand, within thiD at 2447.062 kb on - strand, within thiD at 2447.069 kb on - strand, within thiD at 2447.083 kb on - strand, within thiD at 2447.138 kb on + strand, within thiD at 2447.180 kb on + strand, within thiD at 2447.180 kb on + strand, within thiD at 2447.188 kb on + strand, within thiD at 2447.188 kb on - strand, within thiD at 2447.188 kb on - strand, within thiD at 2447.241 kb on + strand, within thiD at 2447.243 kb on - strand, within thiD at 2447.270 kb on + strand, within thiD at 2447.316 kb on + strand at 2447.316 kb on + strand at 2447.316 kb on + strand at 2447.316 kb on + strand at 2447.316 kb on + strand at 2447.324 kb on - strand at 2447.324 kb on - strand at 2447.324 kb on - strand at 2447.324 kb on - strand at 2447.329 kb on - strand at 2447.334 kb on - strand at 2447.394 kb on - strand, within Synpcc7942_2380 at 2447.448 kb on + strand, within Synpcc7942_2380 at 2447.462 kb on + strand, within Synpcc7942_2380 at 2447.463 kb on - strand, within Synpcc7942_2380 at 2447.464 kb on + strand at 2447.472 kb on - strand, within Synpcc7942_2380 at 2447.510 kb on - strand, within Synpcc7942_2380 at 2447.515 kb on - strand, within Synpcc7942_2380 at 2447.515 kb on - strand, within Synpcc7942_2380 at 2447.534 kb on + strand, within Synpcc7942_2380 at 2447.566 kb on + strand, within Synpcc7942_2380 at 2447.566 kb on + strand, within Synpcc7942_2380 at 2447.574 kb on - strand, within Synpcc7942_2380 at 2447.574 kb on - strand, within Synpcc7942_2380 at 2447.602 kb on - strand, within Synpcc7942_2380 at 2447.620 kb on - strand, within Synpcc7942_2380 at 2447.663 kb on + strand, within Synpcc7942_2380 at 2447.724 kb on - strand, within Synpcc7942_2380 at 2447.727 kb on + strand, within Synpcc7942_2380 at 2447.757 kb on + strand at 2447.775 kb on + strand at 2447.775 kb on - strand at 2447.780 kb on + strand at 2447.788 kb on - strand at 2447.788 kb on - strand at 2447.800 kb on + strand at 2447.806 kb on + strand at 2447.814 kb on + strand at 2447.823 kb on + strand at 2447.829 kb on - strand at 2447.831 kb on - strand at 2447.831 kb on - strand at 2447.831 kb on - strand at 2447.867 kb on - strand at 2447.883 kb on + strand at 2447.885 kb on - strand at 2448.002 kb on + strand at 2448.009 kb on + strand at 2448.009 kb on + strand at 2448.010 kb on - strand at 2448.041 kb on + strand at 2448.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrite 20 mM remove 2,446,571 - +0.5 2,446,583 + -1.5 2,446,591 - -0.5 2,446,651 + thiD Synpcc7942_2379 0.10 -1.0 2,446,692 - thiD Synpcc7942_2379 0.16 -2.3 2,446,699 + thiD Synpcc7942_2379 0.17 +0.4 2,446,702 - thiD Synpcc7942_2379 0.17 +0.0 2,446,702 - thiD Synpcc7942_2379 0.17 -1.2 2,446,712 - thiD Synpcc7942_2379 0.18 +1.4 2,446,712 - thiD Synpcc7942_2379 0.18 +0.9 2,446,722 - thiD Synpcc7942_2379 0.19 -2.4 2,446,769 - thiD Synpcc7942_2379 0.25 -1.4 2,446,805 + thiD Synpcc7942_2379 0.30 -0.3 2,446,810 + thiD Synpcc7942_2379 0.31 -1.5 2,446,863 - thiD Synpcc7942_2379 0.37 +1.5 2,446,865 + thiD Synpcc7942_2379 0.38 +0.9 2,446,902 + thiD Synpcc7942_2379 0.42 -1.6 2,446,902 + thiD Synpcc7942_2379 0.42 -0.4 2,446,907 + thiD Synpcc7942_2379 0.43 -0.0 2,446,907 - thiD Synpcc7942_2379 0.43 +1.1 2,446,956 - thiD Synpcc7942_2379 0.49 +0.7 2,446,956 - thiD Synpcc7942_2379 0.49 -0.3 2,446,975 - thiD Synpcc7942_2379 0.51 +0.2 2,446,989 + thiD Synpcc7942_2379 0.53 -0.7 2,447,003 + thiD Synpcc7942_2379 0.55 -0.3 2,447,015 + thiD Synpcc7942_2379 0.56 -0.5 2,447,035 + thiD Synpcc7942_2379 0.59 -0.5 2,447,050 + thiD Synpcc7942_2379 0.61 +1.1 2,447,050 + thiD Synpcc7942_2379 0.61 +1.3 2,447,054 + thiD Synpcc7942_2379 0.61 -1.4 2,447,062 - thiD Synpcc7942_2379 0.62 -0.0 2,447,069 - thiD Synpcc7942_2379 0.63 +0.8 2,447,083 - thiD Synpcc7942_2379 0.65 -0.4 2,447,138 + thiD Synpcc7942_2379 0.72 -0.6 2,447,180 + thiD Synpcc7942_2379 0.77 -1.9 2,447,180 + thiD Synpcc7942_2379 0.77 +0.4 2,447,188 + thiD Synpcc7942_2379 0.78 +0.7 2,447,188 - thiD Synpcc7942_2379 0.78 -0.2 2,447,188 - thiD Synpcc7942_2379 0.78 +1.0 2,447,241 + thiD Synpcc7942_2379 0.85 -2.1 2,447,243 - thiD Synpcc7942_2379 0.85 -0.3 2,447,270 + thiD Synpcc7942_2379 0.89 -0.3 2,447,316 + -1.2 2,447,316 + +1.2 2,447,316 + +0.5 2,447,316 + +1.0 2,447,316 + +1.0 2,447,324 - +0.5 2,447,324 - -0.1 2,447,324 - +0.3 2,447,324 - -0.2 2,447,329 - -0.0 2,447,334 - +0.6 2,447,394 - Synpcc7942_2380 0.12 +0.2 2,447,448 + Synpcc7942_2380 0.24 +1.5 2,447,462 + Synpcc7942_2380 0.28 -1.9 2,447,463 - Synpcc7942_2380 0.28 -0.4 2,447,464 + -0.5 2,447,472 - Synpcc7942_2380 0.30 -1.2 2,447,510 - Synpcc7942_2380 0.39 -1.7 2,447,515 - Synpcc7942_2380 0.40 +0.0 2,447,515 - Synpcc7942_2380 0.40 -1.3 2,447,534 + Synpcc7942_2380 0.44 -0.9 2,447,566 + Synpcc7942_2380 0.52 -0.4 2,447,566 + Synpcc7942_2380 0.52 -0.2 2,447,574 - Synpcc7942_2380 0.54 -0.1 2,447,574 - Synpcc7942_2380 0.54 -0.8 2,447,602 - Synpcc7942_2380 0.60 -0.5 2,447,620 - Synpcc7942_2380 0.64 +0.8 2,447,663 + Synpcc7942_2380 0.74 -1.4 2,447,724 - Synpcc7942_2380 0.89 -1.2 2,447,727 + Synpcc7942_2380 0.89 -0.9 2,447,757 + -0.8 2,447,775 + +2.9 2,447,775 - -1.8 2,447,780 + -0.4 2,447,788 - -1.2 2,447,788 - -0.2 2,447,800 + -0.9 2,447,806 + -0.1 2,447,814 + +1.0 2,447,823 + -1.5 2,447,829 - +1.8 2,447,831 - +0.9 2,447,831 - +0.3 2,447,831 - +1.4 2,447,867 - -1.1 2,447,883 + -0.5 2,447,885 - -0.3 2,448,002 + +0.4 2,448,009 + -1.0 2,448,009 + +1.0 2,448,010 - -1.8 2,448,041 + -2.4 2,448,067 + -4.0
Or see this region's nucleotide sequence