Experiment: BG11 with nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Synpcc7942_1658 and Synpcc7942_1659 are separated by 137 nucleotides Synpcc7942_1659 and Synpcc7942_1660 are separated by 99 nucleotides Synpcc7942_1660 and digA are separated by 194 nucleotides digA and cysS are separated by 88 nucleotides
Synpcc7942_1658: Synpcc7942_1658 - hypothetical protein, at 1,728,788 to 1,729,246
_1658
Synpcc7942_1659: Synpcc7942_1659 - nitroreductase, at 1,729,384 to 1,730,031
_1659
Synpcc7942_1660: Synpcc7942_1660 - potassium channel protein, at 1,730,131 to 1,730,964
_1660
Synpcc7942_1661: digA - hypothetical protein, at 1,731,159 to 1,731,722
digA
Synpcc7942_1662: cysS - cysteinyl-tRNA synthetase, at 1,731,811 to 1,733,247
cysS
Position (kb)
1730
1731 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1729.163 kb on - strand, within Synpcc7942_1658 at 1729.186 kb on + strand, within Synpcc7942_1658 at 1729.188 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.194 kb on - strand, within Synpcc7942_1658 at 1729.217 kb on - strand at 1729.223 kb on + strand at 1729.223 kb on + strand at 1729.223 kb on + strand at 1729.231 kb on - strand at 1729.244 kb on - strand at 1729.255 kb on - strand at 1729.272 kb on - strand at 1729.293 kb on - strand at 1729.311 kb on + strand at 1729.311 kb on + strand at 1729.319 kb on - strand at 1729.340 kb on + strand at 1729.359 kb on - strand at 1729.359 kb on - strand at 1729.361 kb on + strand at 1729.374 kb on - strand at 1729.387 kb on - strand at 1729.415 kb on - strand at 1729.423 kb on - strand at 1729.483 kb on - strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.496 kb on + strand, within Synpcc7942_1659 at 1729.501 kb on + strand, within Synpcc7942_1659 at 1729.504 kb on - strand, within Synpcc7942_1659 at 1729.504 kb on - strand, within Synpcc7942_1659 at 1729.514 kb on + strand, within Synpcc7942_1659 at 1729.520 kb on - strand, within Synpcc7942_1659 at 1729.531 kb on - strand, within Synpcc7942_1659 at 1729.532 kb on - strand, within Synpcc7942_1659 at 1729.556 kb on + strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.564 kb on - strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.589 kb on + strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.597 kb on - strand, within Synpcc7942_1659 at 1729.599 kb on + strand, within Synpcc7942_1659 at 1729.606 kb on - strand, within Synpcc7942_1659 at 1729.618 kb on - strand, within Synpcc7942_1659 at 1729.651 kb on - strand, within Synpcc7942_1659 at 1729.659 kb on + strand, within Synpcc7942_1659 at 1729.663 kb on - strand, within Synpcc7942_1659 at 1729.678 kb on + strand, within Synpcc7942_1659 at 1729.769 kb on + strand, within Synpcc7942_1659 at 1729.785 kb on + strand, within Synpcc7942_1659 at 1729.785 kb on + strand, within Synpcc7942_1659 at 1729.798 kb on + strand, within Synpcc7942_1659 at 1729.803 kb on + strand, within Synpcc7942_1659 at 1729.803 kb on + strand, within Synpcc7942_1659 at 1729.840 kb on + strand, within Synpcc7942_1659 at 1729.855 kb on + strand, within Synpcc7942_1659 at 1729.908 kb on + strand, within Synpcc7942_1659 at 1729.909 kb on + strand, within Synpcc7942_1659 at 1729.909 kb on + strand, within Synpcc7942_1659 at 1729.915 kb on - strand, within Synpcc7942_1659 at 1729.917 kb on - strand, within Synpcc7942_1659 at 1729.917 kb on - strand, within Synpcc7942_1659 at 1729.933 kb on + strand, within Synpcc7942_1659 at 1729.933 kb on + strand, within Synpcc7942_1659 at 1729.941 kb on - strand, within Synpcc7942_1659 at 1729.941 kb on - strand, within Synpcc7942_1659 at 1729.953 kb on - strand, within Synpcc7942_1659 at 1729.981 kb on + strand at 1729.981 kb on + strand at 1729.981 kb on + strand at 1730.045 kb on + strand at 1730.047 kb on - strand at 1730.047 kb on - strand at 1730.060 kb on + strand at 1730.071 kb on + strand at 1730.079 kb on - strand at 1730.079 kb on - strand at 1730.091 kb on - strand at 1730.123 kb on + strand at 1730.131 kb on - strand at 1730.199 kb on - strand at 1730.199 kb on - strand at 1730.211 kb on - strand at 1730.211 kb on - strand at 1730.212 kb on - strand at 1730.212 kb on - strand at 1730.212 kb on - strand at 1730.262 kb on - strand, within Synpcc7942_1660 at 1730.266 kb on + strand, within Synpcc7942_1660 at 1730.304 kb on - strand, within Synpcc7942_1660 at 1730.304 kb on - strand, within Synpcc7942_1660 at 1730.319 kb on - strand, within Synpcc7942_1660 at 1730.338 kb on - strand, within Synpcc7942_1660 at 1730.374 kb on + strand, within Synpcc7942_1660 at 1730.394 kb on - strand, within Synpcc7942_1660 at 1730.404 kb on - strand, within Synpcc7942_1660 at 1730.441 kb on - strand, within Synpcc7942_1660 at 1730.456 kb on + strand, within Synpcc7942_1660 at 1730.456 kb on + strand, within Synpcc7942_1660 at 1730.464 kb on - strand, within Synpcc7942_1660 at 1730.560 kb on + strand, within Synpcc7942_1660 at 1730.560 kb on + strand, within Synpcc7942_1660 at 1730.570 kb on + strand, within Synpcc7942_1660 at 1730.570 kb on + strand, within Synpcc7942_1660 at 1730.570 kb on + strand, within Synpcc7942_1660 at 1730.575 kb on - strand, within Synpcc7942_1660 at 1730.578 kb on - strand, within Synpcc7942_1660 at 1730.578 kb on - strand, within Synpcc7942_1660 at 1730.657 kb on - strand, within Synpcc7942_1660 at 1730.684 kb on - strand, within Synpcc7942_1660 at 1730.696 kb on - strand, within Synpcc7942_1660 at 1730.754 kb on - strand, within Synpcc7942_1660 at 1730.920 kb on + strand at 1730.936 kb on + strand at 1730.972 kb on - strand at 1731.001 kb on - strand at 1731.195 kb on - strand at 1731.291 kb on + strand, within digA at 1731.291 kb on + strand, within digA at 1731.326 kb on + strand, within digA at 1731.347 kb on + strand, within digA at 1731.374 kb on - strand, within digA at 1731.382 kb on - strand, within digA at 1731.392 kb on - strand, within digA at 1731.414 kb on + strand, within digA at 1731.484 kb on + strand, within digA at 1731.508 kb on + strand, within digA at 1731.524 kb on - strand, within digA at 1731.524 kb on - strand, within digA at 1731.551 kb on + strand, within digA at 1731.559 kb on + strand, within digA at 1731.567 kb on - strand, within digA at 1731.603 kb on + strand, within digA at 1731.608 kb on - strand, within digA at 1731.612 kb on - strand, within digA at 1731.613 kb on - strand, within digA at 1731.635 kb on + strand, within digA at 1731.645 kb on - strand, within digA at 1731.660 kb on + strand, within digA at 1731.660 kb on + strand, within digA at 1731.660 kb on + strand, within digA at 1731.668 kb on - strand at 1731.678 kb on - strand at 1731.678 kb on - strand at 1731.678 kb on - strand at 1731.679 kb on + strand at 1731.687 kb on - strand at 1731.696 kb on + strand at 1731.701 kb on + strand at 1731.720 kb on - strand at 1731.753 kb on - strand at 1731.761 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrite 20 mM remove 1,729,163 - Synpcc7942_1658 0.82 -1.3 1,729,186 + Synpcc7942_1658 0.87 -1.9 1,729,188 - Synpcc7942_1658 0.87 -0.4 1,729,194 - Synpcc7942_1658 0.88 -1.1 1,729,194 - Synpcc7942_1658 0.88 -2.0 1,729,194 - Synpcc7942_1658 0.88 +0.9 1,729,217 - +0.4 1,729,223 + +1.2 1,729,223 + +1.1 1,729,223 + +0.1 1,729,231 - -1.0 1,729,244 - -1.0 1,729,255 - -0.3 1,729,272 - -0.7 1,729,293 - +0.2 1,729,311 + -0.8 1,729,311 + +0.0 1,729,319 - +0.8 1,729,340 + +0.5 1,729,359 - +0.3 1,729,359 - -1.0 1,729,361 + -0.8 1,729,374 - -1.4 1,729,387 - +0.0 1,729,415 - -0.4 1,729,423 - +0.0 1,729,483 - Synpcc7942_1659 0.15 -0.3 1,729,496 + Synpcc7942_1659 0.17 -1.2 1,729,496 + Synpcc7942_1659 0.17 -0.4 1,729,496 + Synpcc7942_1659 0.17 -0.1 1,729,501 + Synpcc7942_1659 0.18 +1.5 1,729,504 - Synpcc7942_1659 0.19 +0.2 1,729,504 - Synpcc7942_1659 0.19 -0.1 1,729,514 + Synpcc7942_1659 0.20 +1.3 1,729,520 - Synpcc7942_1659 0.21 -0.5 1,729,531 - Synpcc7942_1659 0.23 -1.6 1,729,532 - Synpcc7942_1659 0.23 +0.9 1,729,556 + Synpcc7942_1659 0.27 -0.2 1,729,564 - Synpcc7942_1659 0.28 -0.3 1,729,564 - Synpcc7942_1659 0.28 -1.2 1,729,564 - Synpcc7942_1659 0.28 +0.4 1,729,564 - Synpcc7942_1659 0.28 -2.0 1,729,589 + Synpcc7942_1659 0.32 +0.5 1,729,589 + Synpcc7942_1659 0.32 -0.5 1,729,589 + Synpcc7942_1659 0.32 -0.1 1,729,589 + Synpcc7942_1659 0.32 +0.5 1,729,597 - Synpcc7942_1659 0.33 -0.2 1,729,597 - Synpcc7942_1659 0.33 -0.6 1,729,597 - Synpcc7942_1659 0.33 -0.1 1,729,597 - Synpcc7942_1659 0.33 -0.5 1,729,599 + Synpcc7942_1659 0.33 -1.4 1,729,606 - Synpcc7942_1659 0.34 +2.1 1,729,618 - Synpcc7942_1659 0.36 -0.0 1,729,651 - Synpcc7942_1659 0.41 +0.1 1,729,659 + Synpcc7942_1659 0.42 +0.4 1,729,663 - Synpcc7942_1659 0.43 +0.3 1,729,678 + Synpcc7942_1659 0.45 +0.7 1,729,769 + Synpcc7942_1659 0.59 -0.7 1,729,785 + Synpcc7942_1659 0.62 -0.9 1,729,785 + Synpcc7942_1659 0.62 -0.7 1,729,798 + Synpcc7942_1659 0.64 +0.8 1,729,803 + Synpcc7942_1659 0.65 -1.1 1,729,803 + Synpcc7942_1659 0.65 +0.2 1,729,840 + Synpcc7942_1659 0.70 -0.3 1,729,855 + Synpcc7942_1659 0.73 -1.2 1,729,908 + Synpcc7942_1659 0.81 -1.2 1,729,909 + Synpcc7942_1659 0.81 -1.8 1,729,909 + Synpcc7942_1659 0.81 -0.7 1,729,915 - Synpcc7942_1659 0.82 -0.4 1,729,917 - Synpcc7942_1659 0.82 -0.2 1,729,917 - Synpcc7942_1659 0.82 -0.8 1,729,933 + Synpcc7942_1659 0.85 -0.6 1,729,933 + Synpcc7942_1659 0.85 -1.8 1,729,941 - Synpcc7942_1659 0.86 +0.0 1,729,941 - Synpcc7942_1659 0.86 -0.2 1,729,953 - Synpcc7942_1659 0.88 +0.1 1,729,981 + -0.7 1,729,981 + +0.6 1,729,981 + -0.4 1,730,045 + +1.0 1,730,047 - +0.4 1,730,047 - -0.1 1,730,060 + -0.3 1,730,071 + -0.9 1,730,079 - -0.3 1,730,079 - -0.3 1,730,091 - +1.4 1,730,123 + +1.1 1,730,131 - +0.6 1,730,199 - +0.3 1,730,199 - +0.8 1,730,211 - +0.3 1,730,211 - -0.6 1,730,212 - -2.7 1,730,212 - -0.0 1,730,212 - +2.0 1,730,262 - Synpcc7942_1660 0.16 +0.2 1,730,266 + Synpcc7942_1660 0.16 +1.2 1,730,304 - Synpcc7942_1660 0.21 -1.0 1,730,304 - Synpcc7942_1660 0.21 +1.1 1,730,319 - Synpcc7942_1660 0.23 +0.4 1,730,338 - Synpcc7942_1660 0.25 -0.5 1,730,374 + Synpcc7942_1660 0.29 +1.3 1,730,394 - Synpcc7942_1660 0.32 -1.2 1,730,404 - Synpcc7942_1660 0.33 -1.0 1,730,441 - Synpcc7942_1660 0.37 -0.3 1,730,456 + Synpcc7942_1660 0.39 +1.7 1,730,456 + Synpcc7942_1660 0.39 +0.4 1,730,464 - Synpcc7942_1660 0.40 -0.7 1,730,560 + Synpcc7942_1660 0.51 -0.8 1,730,560 + Synpcc7942_1660 0.51 +0.5 1,730,570 + Synpcc7942_1660 0.53 +2.0 1,730,570 + Synpcc7942_1660 0.53 +0.4 1,730,570 + Synpcc7942_1660 0.53 +0.6 1,730,575 - Synpcc7942_1660 0.53 -0.5 1,730,578 - Synpcc7942_1660 0.54 +0.8 1,730,578 - Synpcc7942_1660 0.54 -1.3 1,730,657 - Synpcc7942_1660 0.63 -0.9 1,730,684 - Synpcc7942_1660 0.66 +0.6 1,730,696 - Synpcc7942_1660 0.68 +0.3 1,730,754 - Synpcc7942_1660 0.75 +0.1 1,730,920 + +1.3 1,730,936 + -0.7 1,730,972 - -0.9 1,731,001 - -1.1 1,731,195 - -0.1 1,731,291 + digA Synpcc7942_1661 0.23 -0.4 1,731,291 + digA Synpcc7942_1661 0.23 -0.1 1,731,326 + digA Synpcc7942_1661 0.30 +0.0 1,731,347 + digA Synpcc7942_1661 0.33 -0.3 1,731,374 - digA Synpcc7942_1661 0.38 -3.0 1,731,382 - digA Synpcc7942_1661 0.40 -0.3 1,731,392 - digA Synpcc7942_1661 0.41 -0.9 1,731,414 + digA Synpcc7942_1661 0.45 -2.0 1,731,484 + digA Synpcc7942_1661 0.58 +0.1 1,731,508 + digA Synpcc7942_1661 0.62 +0.8 1,731,524 - digA Synpcc7942_1661 0.65 -1.4 1,731,524 - digA Synpcc7942_1661 0.65 -0.6 1,731,551 + digA Synpcc7942_1661 0.70 -0.2 1,731,559 + digA Synpcc7942_1661 0.71 -0.8 1,731,567 - digA Synpcc7942_1661 0.72 -0.5 1,731,603 + digA Synpcc7942_1661 0.79 +0.3 1,731,608 - digA Synpcc7942_1661 0.80 -1.9 1,731,612 - digA Synpcc7942_1661 0.80 -0.1 1,731,613 - digA Synpcc7942_1661 0.80 -0.1 1,731,635 + digA Synpcc7942_1661 0.84 +0.4 1,731,645 - digA Synpcc7942_1661 0.86 -0.3 1,731,660 + digA Synpcc7942_1661 0.89 +0.4 1,731,660 + digA Synpcc7942_1661 0.89 -0.2 1,731,660 + digA Synpcc7942_1661 0.89 +0.2 1,731,668 - -1.7 1,731,678 - -1.0 1,731,678 - +0.4 1,731,678 - +0.8 1,731,679 + +1.0 1,731,687 - +0.3 1,731,696 + -1.0 1,731,701 + +0.0 1,731,720 - -0.1 1,731,753 - +1.4 1,731,761 + +0.0
Or see this region's nucleotide sequence