Experiment: BG11 with nitrite 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrB and Synpcc7942_0671 are separated by 18 nucleotides Synpcc7942_0671 and rpoD3 are separated by 233 nucleotides rpoD3 and mutT overlap by 1 nucleotides
Synpcc7942_0670: pyrB - aspartate carbamoyltransferase catalytic subunit, at 665,121 to 666,137
pyrB
Synpcc7942_0671: Synpcc7942_0671 - hypothetical protein, at 666,156 to 666,653
_0671
Synpcc7942_0672: rpoD3 - RNA polymerase sigma factor SigD, at 666,887 to 667,849
rpoD3
Synpcc7942_0673: mutT - A/G-specific DNA-adenine glycosylase, at 667,849 to 668,931
mutT
Position (kb) 666
667
668 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 666.080 kb on + strand at 666.106 kb on + strand at 666.109 kb on - strand at 666.114 kb on - strand at 666.119 kb on - strand at 666.159 kb on + strand at 666.202 kb on + strand at 666.205 kb on - strand at 666.205 kb on - strand at 666.210 kb on - strand, within Synpcc7942_0671 at 666.229 kb on + strand, within Synpcc7942_0671 at 666.229 kb on + strand, within Synpcc7942_0671 at 666.252 kb on - strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.256 kb on + strand, within Synpcc7942_0671 at 666.264 kb on - strand, within Synpcc7942_0671 at 666.264 kb on - strand, within Synpcc7942_0671 at 666.324 kb on - strand, within Synpcc7942_0671 at 666.332 kb on + strand, within Synpcc7942_0671 at 666.340 kb on - strand at 666.348 kb on - strand, within Synpcc7942_0671 at 666.377 kb on + strand, within Synpcc7942_0671 at 666.377 kb on + strand, within Synpcc7942_0671 at 666.395 kb on - strand, within Synpcc7942_0671 at 666.396 kb on - strand at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.407 kb on + strand, within Synpcc7942_0671 at 666.414 kb on + strand, within Synpcc7942_0671 at 666.415 kb on - strand, within Synpcc7942_0671 at 666.415 kb on - strand, within Synpcc7942_0671 at 666.422 kb on - strand, within Synpcc7942_0671 at 666.426 kb on + strand, within Synpcc7942_0671 at 666.431 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.476 kb on + strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.484 kb on - strand, within Synpcc7942_0671 at 666.489 kb on - strand, within Synpcc7942_0671 at 666.497 kb on - strand, within Synpcc7942_0671 at 666.499 kb on + strand, within Synpcc7942_0671 at 666.535 kb on + strand, within Synpcc7942_0671 at 666.535 kb on + strand, within Synpcc7942_0671 at 666.538 kb on + strand, within Synpcc7942_0671 at 666.545 kb on + strand at 666.633 kb on - strand at 666.635 kb on + strand at 666.638 kb on - strand at 666.656 kb on - strand at 666.676 kb on + strand at 666.676 kb on - strand at 666.683 kb on + strand at 666.684 kb on - strand at 666.691 kb on - strand at 666.752 kb on - strand at 666.763 kb on - strand at 666.784 kb on - strand at 666.872 kb on + strand at 666.910 kb on + strand at 666.920 kb on + strand at 666.924 kb on + strand at 666.924 kb on + strand at 666.924 kb on + strand at 666.956 kb on + strand at 666.964 kb on - strand at 666.971 kb on + strand at 666.993 kb on + strand, within rpoD3 at 667.000 kb on + strand, within rpoD3 at 667.038 kb on + strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.046 kb on - strand, within rpoD3 at 667.072 kb on + strand, within rpoD3 at 667.106 kb on + strand, within rpoD3 at 667.115 kb on + strand, within rpoD3 at 667.123 kb on + strand, within rpoD3 at 667.178 kb on - strand, within rpoD3 at 667.245 kb on + strand, within rpoD3 at 667.253 kb on - strand, within rpoD3 at 667.370 kb on - strand at 667.385 kb on + strand, within rpoD3 at 667.479 kb on + strand, within rpoD3 at 667.485 kb on + strand, within rpoD3 at 667.485 kb on + strand, within rpoD3 at 667.485 kb on - strand, within rpoD3 at 667.488 kb on + strand, within rpoD3 at 667.493 kb on - strand, within rpoD3 at 667.704 kb on + strand, within rpoD3 at 667.704 kb on + strand, within rpoD3 at 667.704 kb on + strand, within rpoD3 at 667.704 kb on - strand, within rpoD3 at 667.704 kb on - strand, within rpoD3 at 667.706 kb on + strand, within rpoD3 at 667.712 kb on - strand, within rpoD3 at 667.721 kb on + strand, within rpoD3 at 667.729 kb on - strand, within rpoD3 at 667.729 kb on - strand, within rpoD3 at 667.772 kb on - strand at 667.797 kb on + strand at 667.803 kb on - strand at 667.805 kb on - strand at 667.805 kb on - strand at 667.805 kb on - strand at 667.868 kb on + strand at 667.868 kb on + strand at 667.868 kb on + strand at 667.868 kb on + strand at 667.876 kb on - strand at 667.876 kb on - strand at 667.876 kb on - strand at 667.888 kb on + strand at 667.893 kb on + strand at 667.906 kb on + strand at 667.921 kb on - strand at 667.923 kb on + strand at 667.944 kb on + strand at 667.944 kb on + strand at 667.952 kb on - strand at 667.952 kb on - strand at 667.967 kb on + strand, within mutT at 668.016 kb on + strand, within mutT at 668.016 kb on + strand, within mutT at 668.024 kb on - strand, within mutT at 668.024 kb on - strand, within mutT at 668.039 kb on + strand, within mutT at 668.039 kb on + strand, within mutT at 668.047 kb on - strand, within mutT at 668.047 kb on - strand, within mutT at 668.049 kb on - strand, within mutT at 668.055 kb on - strand, within mutT at 668.062 kb on + strand, within mutT at 668.084 kb on - strand, within mutT at 668.092 kb on + strand, within mutT at 668.092 kb on + strand, within mutT at 668.092 kb on + strand, within mutT at 668.110 kb on - strand, within mutT at 668.111 kb on - strand, within mutT at 668.118 kb on + strand, within mutT at 668.119 kb on + strand, within mutT at 668.142 kb on - strand, within mutT at 668.150 kb on - strand, within mutT at 668.163 kb on - strand, within mutT at 668.207 kb on - strand, within mutT at 668.217 kb on - strand, within mutT at 668.219 kb on - strand, within mutT at 668.250 kb on - strand, within mutT at 668.305 kb on + strand, within mutT at 668.313 kb on - strand, within mutT at 668.340 kb on + strand, within mutT at 668.351 kb on + strand, within mutT at 668.351 kb on + strand, within mutT at 668.359 kb on - strand, within mutT at 668.359 kb on - strand, within mutT at 668.364 kb on + strand, within mutT at 668.369 kb on + strand, within mutT at 668.382 kb on - strand, within mutT at 668.387 kb on + strand, within mutT at 668.401 kb on + strand, within mutT at 668.405 kb on - strand, within mutT at 668.409 kb on - strand, within mutT at 668.433 kb on + strand, within mutT at 668.441 kb on - strand at 668.441 kb on - strand, within mutT at 668.441 kb on - strand, within mutT at 668.443 kb on - strand, within mutT at 668.487 kb on - strand, within mutT at 668.496 kb on - strand, within mutT at 668.517 kb on - strand, within mutT at 668.536 kb on - strand, within mutT at 668.591 kb on - strand, within mutT at 668.649 kb on + strand, within mutT at 668.666 kb on + strand, within mutT at 668.667 kb on - strand, within mutT at 668.761 kb on - strand, within mutT
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with nitrite 20 mM remove 666,080 + +0.6 666,106 + -2.5 666,109 - -1.0 666,114 - -0.9 666,119 - -0.5 666,159 + +0.2 666,202 + -1.1 666,205 - -2.0 666,205 - +1.9 666,210 - Synpcc7942_0671 0.11 -2.8 666,229 + Synpcc7942_0671 0.15 +0.3 666,229 + Synpcc7942_0671 0.15 +0.3 666,252 - Synpcc7942_0671 0.19 -1.9 666,256 + Synpcc7942_0671 0.20 +0.2 666,256 + Synpcc7942_0671 0.20 -1.1 666,256 + Synpcc7942_0671 0.20 -0.9 666,256 + Synpcc7942_0671 0.20 -0.7 666,264 - Synpcc7942_0671 0.22 -0.2 666,264 - Synpcc7942_0671 0.22 +0.2 666,324 - Synpcc7942_0671 0.34 +0.3 666,332 + Synpcc7942_0671 0.35 +0.2 666,340 - +0.0 666,348 - Synpcc7942_0671 0.39 +0.2 666,377 + Synpcc7942_0671 0.44 -1.5 666,377 + Synpcc7942_0671 0.44 +0.4 666,395 - Synpcc7942_0671 0.48 +2.3 666,396 - +0.3 666,407 + Synpcc7942_0671 0.50 +0.2 666,407 + Synpcc7942_0671 0.50 +0.9 666,407 + Synpcc7942_0671 0.50 -0.2 666,407 + Synpcc7942_0671 0.50 +0.2 666,414 + Synpcc7942_0671 0.52 +1.1 666,415 - Synpcc7942_0671 0.52 +0.8 666,415 - Synpcc7942_0671 0.52 +1.1 666,422 - Synpcc7942_0671 0.53 -0.1 666,426 + Synpcc7942_0671 0.54 -0.5 666,431 + Synpcc7942_0671 0.55 +0.6 666,476 + Synpcc7942_0671 0.64 +0.7 666,476 + Synpcc7942_0671 0.64 -0.9 666,476 + Synpcc7942_0671 0.64 -1.8 666,484 - Synpcc7942_0671 0.66 -0.4 666,484 - Synpcc7942_0671 0.66 -0.4 666,484 - Synpcc7942_0671 0.66 -0.7 666,489 - Synpcc7942_0671 0.67 +0.1 666,497 - Synpcc7942_0671 0.68 +0.5 666,499 + Synpcc7942_0671 0.69 +0.1 666,535 + Synpcc7942_0671 0.76 -0.9 666,535 + Synpcc7942_0671 0.76 -0.1 666,538 + Synpcc7942_0671 0.77 +0.9 666,545 + +0.3 666,633 - -0.2 666,635 + +0.4 666,638 - -0.0 666,656 - -0.9 666,676 + +0.8 666,676 - -0.5 666,683 + -0.4 666,684 - -0.6 666,691 - +0.2 666,752 - +1.6 666,763 - -0.4 666,784 - -0.5 666,872 + -1.8 666,910 + +1.6 666,920 + -1.0 666,924 + -0.9 666,924 + -0.8 666,924 + -2.4 666,956 + -1.2 666,964 - -1.4 666,971 + -0.3 666,993 + rpoD3 Synpcc7942_0672 0.11 -1.2 667,000 + rpoD3 Synpcc7942_0672 0.12 -3.3 667,038 + rpoD3 Synpcc7942_0672 0.16 -0.5 667,046 - rpoD3 Synpcc7942_0672 0.17 +1.1 667,046 - rpoD3 Synpcc7942_0672 0.17 -2.8 667,046 - rpoD3 Synpcc7942_0672 0.17 -1.4 667,072 + rpoD3 Synpcc7942_0672 0.19 -2.9 667,106 + rpoD3 Synpcc7942_0672 0.23 -1.6 667,115 + rpoD3 Synpcc7942_0672 0.24 -0.1 667,123 + rpoD3 Synpcc7942_0672 0.25 -1.4 667,178 - rpoD3 Synpcc7942_0672 0.30 -1.9 667,245 + rpoD3 Synpcc7942_0672 0.37 -2.6 667,253 - rpoD3 Synpcc7942_0672 0.38 -0.4 667,370 - +0.3 667,385 + rpoD3 Synpcc7942_0672 0.52 -2.2 667,479 + rpoD3 Synpcc7942_0672 0.61 -2.5 667,485 + rpoD3 Synpcc7942_0672 0.62 -3.2 667,485 + rpoD3 Synpcc7942_0672 0.62 -0.9 667,485 - rpoD3 Synpcc7942_0672 0.62 +0.3 667,488 + rpoD3 Synpcc7942_0672 0.62 -3.6 667,493 - rpoD3 Synpcc7942_0672 0.63 +0.9 667,704 + rpoD3 Synpcc7942_0672 0.85 -1.7 667,704 + rpoD3 Synpcc7942_0672 0.85 -0.8 667,704 + rpoD3 Synpcc7942_0672 0.85 -3.4 667,704 - rpoD3 Synpcc7942_0672 0.85 -2.1 667,704 - rpoD3 Synpcc7942_0672 0.85 -2.0 667,706 + rpoD3 Synpcc7942_0672 0.85 -1.0 667,712 - rpoD3 Synpcc7942_0672 0.86 -1.4 667,721 + rpoD3 Synpcc7942_0672 0.87 -1.7 667,729 - rpoD3 Synpcc7942_0672 0.87 -2.6 667,729 - rpoD3 Synpcc7942_0672 0.87 -2.1 667,772 - +0.5 667,797 + -1.5 667,803 - -0.5 667,805 - -2.2 667,805 - -3.0 667,805 - -1.4 667,868 + -1.0 667,868 + -1.0 667,868 + -1.1 667,868 + -0.4 667,876 - -0.9 667,876 - -0.6 667,876 - -0.1 667,888 + -0.2 667,893 + -2.4 667,906 + -0.6 667,921 - -0.9 667,923 + +0.7 667,944 + -0.1 667,944 + -1.1 667,952 - -1.6 667,952 - +0.6 667,967 + mutT Synpcc7942_0673 0.11 -0.7 668,016 + mutT Synpcc7942_0673 0.15 +0.0 668,016 + mutT Synpcc7942_0673 0.15 +0.8 668,024 - mutT Synpcc7942_0673 0.16 -0.7 668,024 - mutT Synpcc7942_0673 0.16 -0.9 668,039 + mutT Synpcc7942_0673 0.18 -1.2 668,039 + mutT Synpcc7942_0673 0.18 -0.1 668,047 - mutT Synpcc7942_0673 0.18 -2.2 668,047 - mutT Synpcc7942_0673 0.18 -0.2 668,049 - mutT Synpcc7942_0673 0.18 +0.8 668,055 - mutT Synpcc7942_0673 0.19 -0.3 668,062 + mutT Synpcc7942_0673 0.20 +0.7 668,084 - mutT Synpcc7942_0673 0.22 -0.2 668,092 + mutT Synpcc7942_0673 0.22 +2.2 668,092 + mutT Synpcc7942_0673 0.22 -0.2 668,092 + mutT Synpcc7942_0673 0.22 +0.0 668,110 - mutT Synpcc7942_0673 0.24 -1.1 668,111 - mutT Synpcc7942_0673 0.24 -3.1 668,118 + mutT Synpcc7942_0673 0.25 +0.5 668,119 + mutT Synpcc7942_0673 0.25 -0.5 668,142 - mutT Synpcc7942_0673 0.27 +0.2 668,150 - mutT Synpcc7942_0673 0.28 +0.1 668,163 - mutT Synpcc7942_0673 0.29 -0.2 668,207 - mutT Synpcc7942_0673 0.33 -1.2 668,217 - mutT Synpcc7942_0673 0.34 -2.4 668,219 - mutT Synpcc7942_0673 0.34 +0.8 668,250 - mutT Synpcc7942_0673 0.37 -1.3 668,305 + mutT Synpcc7942_0673 0.42 +0.8 668,313 - mutT Synpcc7942_0673 0.43 -2.4 668,340 + mutT Synpcc7942_0673 0.45 -2.8 668,351 + mutT Synpcc7942_0673 0.46 -0.1 668,351 + mutT Synpcc7942_0673 0.46 -0.7 668,359 - mutT Synpcc7942_0673 0.47 -0.3 668,359 - mutT Synpcc7942_0673 0.47 -1.7 668,364 + mutT Synpcc7942_0673 0.48 +0.6 668,369 + mutT Synpcc7942_0673 0.48 -1.0 668,382 - mutT Synpcc7942_0673 0.49 -0.7 668,387 + mutT Synpcc7942_0673 0.50 -0.1 668,401 + mutT Synpcc7942_0673 0.51 +1.3 668,405 - mutT Synpcc7942_0673 0.51 -0.9 668,409 - mutT Synpcc7942_0673 0.52 +0.1 668,433 + mutT Synpcc7942_0673 0.54 +0.4 668,441 - +1.9 668,441 - mutT Synpcc7942_0673 0.55 -0.4 668,441 - mutT Synpcc7942_0673 0.55 +0.8 668,443 - mutT Synpcc7942_0673 0.55 -0.6 668,487 - mutT Synpcc7942_0673 0.59 -1.8 668,496 - mutT Synpcc7942_0673 0.60 +0.3 668,517 - mutT Synpcc7942_0673 0.62 +0.5 668,536 - mutT Synpcc7942_0673 0.63 -0.5 668,591 - mutT Synpcc7942_0673 0.69 +0.7 668,649 + mutT Synpcc7942_0673 0.74 +0.9 668,666 + mutT Synpcc7942_0673 0.75 +0.9 668,667 - mutT Synpcc7942_0673 0.76 -0.8 668,761 - mutT Synpcc7942_0673 0.84 +0.3
Or see this region's nucleotide sequence