Strain Fitness in Paraburkholderia bryophila 376MFSha3.1 around H281DRAFT_01052

Experiment: Dodecandioic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntH281DRAFT_01050 and H281DRAFT_01051 overlap by 4 nucleotidesH281DRAFT_01051 and H281DRAFT_01052 are separated by 27 nucleotidesH281DRAFT_01052 and H281DRAFT_01053 are separated by 34 nucleotidesH281DRAFT_01053 and H281DRAFT_01054 are separated by 17 nucleotides H281DRAFT_01050: H281DRAFT_01050 - maleylacetate reductase, at 1,180,503 to 1,181,597 _01050 H281DRAFT_01051: H281DRAFT_01051 - hydroxyquinol 1,2-dioxygenase, at 1,181,594 to 1,182,454 _01051 H281DRAFT_01052: H281DRAFT_01052 - hypothetical protein, at 1,182,482 to 1,182,766 _01052 H281DRAFT_01053: H281DRAFT_01053 - 3-hydroxyisobutyrate dehydrogenase, at 1,182,801 to 1,183,706 _01053 H281DRAFT_01054: H281DRAFT_01054 - (R,R)-butanediol dehydrogenase / meso-butanediol dehydrogenase / diacetyl reductase, at 1,183,724 to 1,184,794 _01054 Position (kb) 1182 1183Strain fitness (log2 ratio) -2 -1 0 1at 1182.706 kb on - strand, within H281DRAFT_01052at 1182.760 kb on - strandat 1182.760 kb on - strandat 1182.766 kb on + strandat 1182.881 kb on - strandat 1183.412 kb on - strand, within H281DRAFT_01053at 1183.594 kb on + strand, within H281DRAFT_01053

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Per-strain Table

Position Strand Gene LocusTag Fraction Dodecandioic (C)
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1,182,706 - H281DRAFT_01052 0.79 +0.9
1,182,760 - -0.6
1,182,760 - +0.5
1,182,766 + -1.3
1,182,881 - -1.6
1,183,412 - H281DRAFT_01053 0.67 +0.4
1,183,594 + H281DRAFT_01053 0.88 -2.3

Or see this region's nucleotide sequence