Experiment: Cerium(III) chloride 0.000195313 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4539 and Pf6N2E2_4540 are separated by 419 nucleotides Pf6N2E2_4540 and Pf6N2E2_4541 are separated by 124 nucleotides Pf6N2E2_4541 and Pf6N2E2_4542 are separated by 206 nucleotides
Pf6N2E2_4539 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 5,272,146 to 5,273,246
_4539
Pf6N2E2_4540 - hypothetical protein, at 5,273,666 to 5,273,881
_4540
Pf6N2E2_4541 - hypothetical protein, at 5,274,006 to 5,274,455
_4541
Pf6N2E2_4542 - negative regulator of hrp expression HrpV, at 5,274,662 to 5,275,039
_4542
Position (kb) 5273
5274 Strain fitness (log2 ratio) -2
-1
0
1 at 5272.680 kb on + strand, within Pf6N2E2_4539 at 5272.680 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.735 kb on + strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.736 kb on - strand, within Pf6N2E2_4539 at 5272.776 kb on + strand, within Pf6N2E2_4539 at 5272.776 kb on + strand, within Pf6N2E2_4539 at 5272.855 kb on + strand, within Pf6N2E2_4539 at 5272.855 kb on + strand, within Pf6N2E2_4539 at 5272.980 kb on + strand, within Pf6N2E2_4539 at 5272.980 kb on + strand, within Pf6N2E2_4539 at 5272.981 kb on - strand, within Pf6N2E2_4539 at 5273.113 kb on - strand, within Pf6N2E2_4539 at 5273.133 kb on + strand, within Pf6N2E2_4539 at 5273.173 kb on - strand at 5273.266 kb on + strand at 5273.394 kb on + strand at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.761 kb on + strand, within Pf6N2E2_4540 at 5273.762 kb on - strand, within Pf6N2E2_4540 at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.897 kb on + strand at 5273.898 kb on - strand at 5273.999 kb on + strand at 5273.999 kb on + strand at 5274.000 kb on - strand at 5274.362 kb on - strand, within Pf6N2E2_4541 at 5274.471 kb on + strand at 5274.471 kb on + strand at 5274.471 kb on + strand at 5274.472 kb on - strand at 5274.666 kb on + strand at 5274.666 kb on + strand at 5274.667 kb on - strand at 5274.678 kb on + strand at 5274.678 kb on + strand at 5274.679 kb on - strand at 5274.806 kb on + strand, within Pf6N2E2_4542 at 5274.806 kb on + strand, within Pf6N2E2_4542 at 5274.807 kb on - strand, within Pf6N2E2_4542 at 5274.807 kb on - strand, within Pf6N2E2_4542 at 5274.822 kb on + strand, within Pf6N2E2_4542
Per-strain Table
Position Strand Gene LocusTag Fraction Cerium(III) chloride 0.000195313 M remove 5,272,680 + Pf6N2E2_4539 0.49 +0.3 5,272,680 + Pf6N2E2_4539 0.49 -0.4 5,272,735 + Pf6N2E2_4539 0.53 -0.3 5,272,735 + Pf6N2E2_4539 0.53 -0.0 5,272,735 + Pf6N2E2_4539 0.53 +1.2 5,272,735 + Pf6N2E2_4539 0.53 +0.8 5,272,735 + Pf6N2E2_4539 0.53 -0.0 5,272,735 + Pf6N2E2_4539 0.53 -1.5 5,272,736 - Pf6N2E2_4539 0.54 -0.3 5,272,736 - Pf6N2E2_4539 0.54 +1.1 5,272,736 - Pf6N2E2_4539 0.54 +0.1 5,272,736 - Pf6N2E2_4539 0.54 +0.3 5,272,776 + Pf6N2E2_4539 0.57 -0.6 5,272,776 + Pf6N2E2_4539 0.57 -0.4 5,272,855 + Pf6N2E2_4539 0.64 -1.1 5,272,855 + Pf6N2E2_4539 0.64 -0.3 5,272,980 + Pf6N2E2_4539 0.76 +0.5 5,272,980 + Pf6N2E2_4539 0.76 -0.8 5,272,981 - Pf6N2E2_4539 0.76 +0.3 5,273,113 - Pf6N2E2_4539 0.88 +1.1 5,273,133 + Pf6N2E2_4539 0.90 -1.4 5,273,173 - +0.1 5,273,266 + +0.6 5,273,394 + -2.6 5,273,761 + Pf6N2E2_4540 0.44 -0.9 5,273,761 + Pf6N2E2_4540 0.44 +0.9 5,273,761 + Pf6N2E2_4540 0.44 +1.0 5,273,762 - Pf6N2E2_4540 0.44 -0.2 5,273,897 + -0.8 5,273,897 + +0.5 5,273,897 + +0.4 5,273,898 - -0.8 5,273,999 + -1.0 5,273,999 + +0.5 5,274,000 - +0.6 5,274,362 - Pf6N2E2_4541 0.79 +1.2 5,274,471 + +0.2 5,274,471 + +0.8 5,274,471 + -0.5 5,274,472 - -0.0 5,274,666 + +0.0 5,274,666 + -0.3 5,274,667 - -0.4 5,274,678 + -0.2 5,274,678 + +0.3 5,274,679 - -1.8 5,274,806 + Pf6N2E2_4542 0.38 -0.4 5,274,806 + Pf6N2E2_4542 0.38 +0.0 5,274,807 - Pf6N2E2_4542 0.38 +0.8 5,274,807 - Pf6N2E2_4542 0.38 -0.2 5,274,822 + Pf6N2E2_4542 0.42 -0.4
Or see this region's nucleotide sequence