Experiment: PYE 4C survival rep2 58 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00708 and CCNA_00709 are separated by 107 nucleotides CCNA_00709 and CCNA_00710 are separated by 120 nucleotides CCNA_00710 and CCNA_00711 are separated by 142 nucleotides
CCNA_00708: CCNA_00708 - cobaltochelatase cobT subunit, at 765,182 to 767,110
_00708
CCNA_00709: CCNA_00709 - hypothetical protein, at 767,218 to 767,400
_00709
CCNA_00710: CCNA_00710 - alpha/beta hydrolase family protein, at 767,521 to 768,690
_00710
CCNA_00711: CCNA_00711 - hypothetical protein, at 768,833 to 769,933
_00711
Position (kb)
767
768
769 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 766.556 kb on + strand, within CCNA_00708 at 766.564 kb on - strand, within CCNA_00708 at 766.564 kb on - strand, within CCNA_00708 at 766.564 kb on - strand, within CCNA_00708 at 766.564 kb on - strand, within CCNA_00708 at 766.580 kb on - strand, within CCNA_00708 at 766.697 kb on - strand, within CCNA_00708 at 766.718 kb on - strand, within CCNA_00708 at 766.851 kb on - strand, within CCNA_00708 at 766.873 kb on - strand, within CCNA_00708 at 767.067 kb on - strand at 767.078 kb on - strand at 767.078 kb on - strand at 767.119 kb on + strand at 767.209 kb on - strand at 767.220 kb on + strand at 767.246 kb on + strand, within CCNA_00709 at 767.264 kb on - strand, within CCNA_00709 at 767.296 kb on + strand, within CCNA_00709 at 767.322 kb on - strand, within CCNA_00709 at 767.339 kb on + strand, within CCNA_00709 at 767.371 kb on + strand, within CCNA_00709 at 767.371 kb on + strand, within CCNA_00709 at 767.371 kb on + strand, within CCNA_00709 at 767.371 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.373 kb on + strand, within CCNA_00709 at 767.379 kb on - strand, within CCNA_00709 at 767.379 kb on - strand, within CCNA_00709 at 767.381 kb on - strand, within CCNA_00709 at 767.461 kb on - strand at 767.496 kb on - strand at 767.531 kb on + strand at 767.537 kb on + strand at 767.539 kb on - strand at 767.545 kb on - strand at 767.545 kb on - strand at 767.576 kb on - strand at 767.581 kb on - strand at 767.583 kb on + strand at 767.591 kb on - strand at 767.598 kb on + strand at 767.599 kb on + strand at 767.606 kb on + strand at 767.621 kb on - strand at 767.645 kb on + strand, within CCNA_00710 at 767.650 kb on + strand, within CCNA_00710 at 767.653 kb on - strand, within CCNA_00710 at 767.658 kb on - strand, within CCNA_00710 at 767.689 kb on - strand, within CCNA_00710 at 767.697 kb on + strand, within CCNA_00710 at 767.713 kb on + strand, within CCNA_00710 at 767.716 kb on - strand, within CCNA_00710 at 767.773 kb on + strand, within CCNA_00710 at 767.773 kb on + strand, within CCNA_00710 at 767.801 kb on - strand, within CCNA_00710 at 767.838 kb on + strand, within CCNA_00710 at 767.843 kb on + strand, within CCNA_00710 at 767.851 kb on - strand, within CCNA_00710 at 767.851 kb on - strand, within CCNA_00710 at 767.869 kb on + strand, within CCNA_00710 at 767.870 kb on - strand, within CCNA_00710 at 767.872 kb on - strand, within CCNA_00710 at 767.872 kb on - strand, within CCNA_00710 at 767.872 kb on - strand, within CCNA_00710 at 767.942 kb on - strand, within CCNA_00710 at 767.979 kb on + strand, within CCNA_00710 at 767.979 kb on + strand, within CCNA_00710 at 767.979 kb on + strand, within CCNA_00710 at 767.979 kb on + strand, within CCNA_00710 at 767.979 kb on + strand, within CCNA_00710 at 767.979 kb on + strand, within CCNA_00710 at 767.979 kb on + strand, within CCNA_00710 at 767.987 kb on - strand, within CCNA_00710 at 767.987 kb on - strand, within CCNA_00710 at 767.987 kb on - strand, within CCNA_00710 at 767.987 kb on - strand, within CCNA_00710 at 767.987 kb on - strand, within CCNA_00710 at 767.987 kb on - strand, within CCNA_00710 at 768.001 kb on + strand, within CCNA_00710 at 768.040 kb on - strand, within CCNA_00710 at 768.083 kb on + strand, within CCNA_00710 at 768.091 kb on - strand, within CCNA_00710 at 768.101 kb on - strand, within CCNA_00710 at 768.124 kb on + strand, within CCNA_00710 at 768.158 kb on - strand, within CCNA_00710 at 768.160 kb on - strand, within CCNA_00710 at 768.211 kb on + strand, within CCNA_00710 at 768.214 kb on - strand, within CCNA_00710 at 768.238 kb on + strand, within CCNA_00710 at 768.238 kb on + strand, within CCNA_00710 at 768.241 kb on - strand, within CCNA_00710 at 768.246 kb on - strand, within CCNA_00710 at 768.273 kb on + strand, within CCNA_00710 at 768.310 kb on + strand, within CCNA_00710 at 768.342 kb on - strand, within CCNA_00710 at 768.342 kb on - strand, within CCNA_00710 at 768.347 kb on - strand, within CCNA_00710 at 768.418 kb on + strand, within CCNA_00710 at 768.426 kb on - strand, within CCNA_00710 at 768.448 kb on + strand, within CCNA_00710 at 768.456 kb on - strand, within CCNA_00710 at 768.456 kb on - strand, within CCNA_00710 at 768.456 kb on - strand, within CCNA_00710 at 768.456 kb on - strand, within CCNA_00710 at 768.456 kb on - strand, within CCNA_00710 at 768.470 kb on + strand, within CCNA_00710 at 768.478 kb on - strand, within CCNA_00710 at 768.478 kb on - strand, within CCNA_00710 at 768.478 kb on - strand, within CCNA_00710 at 768.478 kb on - strand, within CCNA_00710 at 768.478 kb on - strand, within CCNA_00710 at 768.478 kb on - strand, within CCNA_00710 at 768.486 kb on - strand, within CCNA_00710 at 768.487 kb on - strand, within CCNA_00710 at 768.493 kb on - strand, within CCNA_00710 at 768.515 kb on + strand, within CCNA_00710 at 768.528 kb on - strand, within CCNA_00710 at 768.541 kb on - strand, within CCNA_00710 at 768.562 kb on - strand, within CCNA_00710 at 768.603 kb on + strand at 768.645 kb on + strand at 768.645 kb on + strand at 768.663 kb on + strand at 768.762 kb on + strand at 768.770 kb on - strand at 768.821 kb on - strand at 768.830 kb on + strand at 768.830 kb on + strand at 768.840 kb on + strand at 768.904 kb on + strand at 768.905 kb on + strand at 768.910 kb on + strand at 768.947 kb on + strand, within CCNA_00711 at 768.947 kb on + strand, within CCNA_00711 at 768.963 kb on + strand at 768.963 kb on + strand at 768.963 kb on + strand, within CCNA_00711 at 768.971 kb on - strand, within CCNA_00711 at 768.976 kb on + strand, within CCNA_00711 at 768.993 kb on - strand, within CCNA_00711 at 769.027 kb on + strand, within CCNA_00711 at 769.027 kb on + strand, within CCNA_00711 at 769.035 kb on - strand, within CCNA_00711 at 769.047 kb on + strand, within CCNA_00711 at 769.049 kb on + strand, within CCNA_00711 at 769.050 kb on - strand, within CCNA_00711 at 769.057 kb on - strand, within CCNA_00711 at 769.057 kb on - strand, within CCNA_00711 at 769.059 kb on + strand, within CCNA_00711 at 769.119 kb on + strand, within CCNA_00711 at 769.162 kb on - strand, within CCNA_00711 at 769.165 kb on + strand, within CCNA_00711 at 769.165 kb on + strand at 769.165 kb on + strand, within CCNA_00711 at 769.165 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.195 kb on + strand, within CCNA_00711 at 769.203 kb on - strand, within CCNA_00711 at 769.203 kb on - strand, within CCNA_00711 at 769.203 kb on - strand, within CCNA_00711 at 769.203 kb on - strand, within CCNA_00711 at 769.203 kb on - strand, within CCNA_00711 at 769.225 kb on + strand, within CCNA_00711 at 769.225 kb on + strand, within CCNA_00711 at 769.225 kb on + strand, within CCNA_00711 at 769.233 kb on - strand, within CCNA_00711 at 769.233 kb on - strand, within CCNA_00711 at 769.233 kb on - strand, within CCNA_00711 at 769.233 kb on - strand, within CCNA_00711 at 769.238 kb on - strand, within CCNA_00711 at 769.244 kb on + strand, within CCNA_00711 at 769.255 kb on + strand, within CCNA_00711 at 769.255 kb on + strand, within CCNA_00711 at 769.255 kb on + strand, within CCNA_00711 at 769.255 kb on + strand, within CCNA_00711 at 769.255 kb on + strand, within CCNA_00711 at 769.263 kb on - strand, within CCNA_00711 at 769.263 kb on - strand, within CCNA_00711 at 769.263 kb on - strand, within CCNA_00711 at 769.263 kb on - strand, within CCNA_00711 at 769.263 kb on - strand, within CCNA_00711 at 769.263 kb on - strand, within CCNA_00711 at 769.263 kb on - strand, within CCNA_00711 at 769.305 kb on - strand, within CCNA_00711 at 769.322 kb on + strand, within CCNA_00711 at 769.330 kb on + strand, within CCNA_00711 at 769.345 kb on + strand, within CCNA_00711 at 769.353 kb on - strand, within CCNA_00711 at 769.367 kb on - strand, within CCNA_00711 at 769.415 kb on + strand, within CCNA_00711 at 769.451 kb on + strand, within CCNA_00711 at 769.451 kb on + strand, within CCNA_00711 at 769.456 kb on + strand, within CCNA_00711 at 769.459 kb on - strand, within CCNA_00711 at 769.461 kb on + strand, within CCNA_00711 at 769.461 kb on + strand, within CCNA_00711 at 769.464 kb on - strand, within CCNA_00711 at 769.468 kb on + strand, within CCNA_00711 at 769.468 kb on + strand, within CCNA_00711 at 769.468 kb on + strand, within CCNA_00711 at 769.469 kb on - strand, within CCNA_00711 at 769.469 kb on - strand, within CCNA_00711 at 769.469 kb on - strand, within CCNA_00711 at 769.469 kb on - strand, within CCNA_00711 at 769.498 kb on + strand, within CCNA_00711 at 769.500 kb on + strand, within CCNA_00711 at 769.500 kb on + strand, within CCNA_00711 at 769.508 kb on - strand, within CCNA_00711 at 769.589 kb on - strand, within CCNA_00711 at 769.591 kb on + strand, within CCNA_00711 at 769.591 kb on + strand, within CCNA_00711 at 769.591 kb on + strand, within CCNA_00711 at 769.591 kb on + strand, within CCNA_00711 at 769.591 kb on + strand, within CCNA_00711 at 769.599 kb on - strand, within CCNA_00711 at 769.599 kb on - strand, within CCNA_00711 at 769.599 kb on - strand, within CCNA_00711 at 769.631 kb on - strand, within CCNA_00711 at 769.652 kb on + strand, within CCNA_00711 at 769.660 kb on - strand, within CCNA_00711 at 769.674 kb on + strand, within CCNA_00711 at 769.685 kb on - strand, within CCNA_00711
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 4C survival rep2 58 days remove 766,556 + CCNA_00708 0.71 -0.4 766,564 - CCNA_00708 0.72 +1.0 766,564 - CCNA_00708 0.72 -1.8 766,564 - CCNA_00708 0.72 +0.6 766,564 - CCNA_00708 0.72 -1.0 766,580 - CCNA_00708 0.72 +1.6 766,697 - CCNA_00708 0.79 +0.0 766,718 - CCNA_00708 0.80 -2.0 766,851 - CCNA_00708 0.87 -1.9 766,873 - CCNA_00708 0.88 -1.6 767,067 - -0.6 767,078 - +0.2 767,078 - -3.4 767,119 + -2.0 767,209 - +1.1 767,220 + +0.4 767,246 + CCNA_00709 0.15 +1.2 767,264 - CCNA_00709 0.25 -0.6 767,296 + CCNA_00709 0.43 -0.0 767,322 - CCNA_00709 0.57 -0.9 767,339 + CCNA_00709 0.66 -0.1 767,371 + CCNA_00709 0.84 -0.7 767,371 + CCNA_00709 0.84 +1.5 767,371 + CCNA_00709 0.84 -1.2 767,371 + CCNA_00709 0.84 +0.0 767,373 + CCNA_00709 0.85 +0.9 767,373 + CCNA_00709 0.85 -1.5 767,373 + CCNA_00709 0.85 +1.3 767,373 + CCNA_00709 0.85 +0.3 767,373 + CCNA_00709 0.85 +0.6 767,373 + CCNA_00709 0.85 -2.7 767,373 + CCNA_00709 0.85 +0.3 767,373 + CCNA_00709 0.85 +1.1 767,373 + CCNA_00709 0.85 +0.4 767,373 + CCNA_00709 0.85 +0.6 767,379 - CCNA_00709 0.88 +0.1 767,379 - CCNA_00709 0.88 -1.4 767,381 - CCNA_00709 0.89 -1.0 767,461 - +0.5 767,496 - +2.6 767,531 + +2.0 767,537 + +2.6 767,539 - -0.5 767,545 - -0.7 767,545 - -1.4 767,576 - +0.5 767,581 - +1.1 767,583 + -1.8 767,591 - -0.0 767,598 + +4.1 767,599 + +1.9 767,606 + -1.1 767,621 - -1.6 767,645 + CCNA_00710 0.11 +0.5 767,650 + CCNA_00710 0.11 -0.5 767,653 - CCNA_00710 0.11 +0.6 767,658 - CCNA_00710 0.12 -0.2 767,689 - CCNA_00710 0.14 -0.6 767,697 + CCNA_00710 0.15 +0.9 767,713 + CCNA_00710 0.16 -1.4 767,716 - CCNA_00710 0.17 -0.9 767,773 + CCNA_00710 0.22 +0.1 767,773 + CCNA_00710 0.22 +0.0 767,801 - CCNA_00710 0.24 +0.6 767,838 + CCNA_00710 0.27 -0.3 767,843 + CCNA_00710 0.28 -1.8 767,851 - CCNA_00710 0.28 +0.9 767,851 - CCNA_00710 0.28 +2.9 767,869 + CCNA_00710 0.30 +0.1 767,870 - CCNA_00710 0.30 +0.6 767,872 - CCNA_00710 0.30 +0.4 767,872 - CCNA_00710 0.30 -1.5 767,872 - CCNA_00710 0.30 +1.2 767,942 - CCNA_00710 0.36 -0.9 767,979 + CCNA_00710 0.39 -0.7 767,979 + CCNA_00710 0.39 -1.0 767,979 + CCNA_00710 0.39 -1.1 767,979 + CCNA_00710 0.39 -0.3 767,979 + CCNA_00710 0.39 -0.2 767,979 + CCNA_00710 0.39 +0.6 767,979 + CCNA_00710 0.39 -0.2 767,987 - CCNA_00710 0.40 +0.1 767,987 - CCNA_00710 0.40 -1.5 767,987 - CCNA_00710 0.40 +0.9 767,987 - CCNA_00710 0.40 +1.1 767,987 - CCNA_00710 0.40 +0.2 767,987 - CCNA_00710 0.40 +0.1 768,001 + CCNA_00710 0.41 -0.7 768,040 - CCNA_00710 0.44 -1.5 768,083 + CCNA_00710 0.48 -0.2 768,091 - CCNA_00710 0.49 -0.1 768,101 - CCNA_00710 0.50 -0.6 768,124 + CCNA_00710 0.52 +3.6 768,158 - CCNA_00710 0.54 +0.5 768,160 - CCNA_00710 0.55 -0.4 768,211 + CCNA_00710 0.59 +0.2 768,214 - CCNA_00710 0.59 -1.0 768,238 + CCNA_00710 0.61 +0.5 768,238 + CCNA_00710 0.61 +1.6 768,241 - CCNA_00710 0.62 -2.0 768,246 - CCNA_00710 0.62 +2.6 768,273 + CCNA_00710 0.64 -0.5 768,310 + CCNA_00710 0.67 -2.4 768,342 - CCNA_00710 0.70 +1.4 768,342 - CCNA_00710 0.70 -0.2 768,347 - CCNA_00710 0.71 -1.7 768,418 + CCNA_00710 0.77 +0.4 768,426 - CCNA_00710 0.77 -0.9 768,448 + CCNA_00710 0.79 -0.1 768,456 - CCNA_00710 0.80 -0.9 768,456 - CCNA_00710 0.80 +0.5 768,456 - CCNA_00710 0.80 -0.9 768,456 - CCNA_00710 0.80 -1.2 768,456 - CCNA_00710 0.80 +2.6 768,470 + CCNA_00710 0.81 -0.5 768,478 - CCNA_00710 0.82 +0.3 768,478 - CCNA_00710 0.82 +1.7 768,478 - CCNA_00710 0.82 -0.2 768,478 - CCNA_00710 0.82 -0.1 768,478 - CCNA_00710 0.82 +0.1 768,478 - CCNA_00710 0.82 -1.3 768,486 - CCNA_00710 0.82 -1.7 768,487 - CCNA_00710 0.83 +0.8 768,493 - CCNA_00710 0.83 +1.6 768,515 + CCNA_00710 0.85 +1.0 768,528 - CCNA_00710 0.86 +1.3 768,541 - CCNA_00710 0.87 -2.2 768,562 - CCNA_00710 0.89 -0.7 768,603 + -1.9 768,645 + +1.0 768,645 + -1.2 768,663 + -1.0 768,762 + -0.7 768,770 - -2.1 768,821 - +0.3 768,830 + -0.7 768,830 + -3.2 768,840 + -2.1 768,904 + -2.5 768,905 + +4.2 768,910 + +2.6 768,947 + CCNA_00711 0.10 +1.6 768,947 + CCNA_00711 0.10 -0.2 768,963 + +1.0 768,963 + -1.9 768,963 + CCNA_00711 0.12 +0.8 768,971 - CCNA_00711 0.13 +2.6 768,976 + CCNA_00711 0.13 -1.2 768,993 - CCNA_00711 0.15 -2.6 769,027 + CCNA_00711 0.18 +2.0 769,027 + CCNA_00711 0.18 +1.0 769,035 - CCNA_00711 0.18 +0.0 769,047 + CCNA_00711 0.19 +1.2 769,049 + CCNA_00711 0.20 -0.2 769,050 - CCNA_00711 0.20 -1.7 769,057 - CCNA_00711 0.20 +1.6 769,057 - CCNA_00711 0.20 -0.8 769,059 + CCNA_00711 0.21 +0.6 769,119 + CCNA_00711 0.26 +0.3 769,162 - CCNA_00711 0.30 +0.0 769,165 + CCNA_00711 0.30 +0.6 769,165 + +2.6 769,165 + CCNA_00711 0.30 -0.3 769,165 + CCNA_00711 0.30 -1.1 769,195 + CCNA_00711 0.33 -0.7 769,195 + CCNA_00711 0.33 -0.4 769,195 + CCNA_00711 0.33 +0.3 769,195 + CCNA_00711 0.33 -1.9 769,195 + CCNA_00711 0.33 +1.1 769,195 + CCNA_00711 0.33 +0.3 769,195 + CCNA_00711 0.33 +0.2 769,195 + CCNA_00711 0.33 +0.1 769,195 + CCNA_00711 0.33 -1.8 769,195 + CCNA_00711 0.33 -0.9 769,203 - CCNA_00711 0.34 -0.4 769,203 - CCNA_00711 0.34 +0.5 769,203 - CCNA_00711 0.34 -0.7 769,203 - CCNA_00711 0.34 -2.1 769,203 - CCNA_00711 0.34 -2.9 769,225 + CCNA_00711 0.36 -1.2 769,225 + CCNA_00711 0.36 +1.6 769,225 + CCNA_00711 0.36 +1.3 769,233 - CCNA_00711 0.36 -0.2 769,233 - CCNA_00711 0.36 +1.0 769,233 - CCNA_00711 0.36 -1.2 769,233 - CCNA_00711 0.36 -0.9 769,238 - CCNA_00711 0.37 -2.1 769,244 + CCNA_00711 0.37 -0.9 769,255 + CCNA_00711 0.38 +0.6 769,255 + CCNA_00711 0.38 +0.0 769,255 + CCNA_00711 0.38 -2.5 769,255 + CCNA_00711 0.38 +0.3 769,255 + CCNA_00711 0.38 -0.4 769,263 - CCNA_00711 0.39 -0.9 769,263 - CCNA_00711 0.39 -0.4 769,263 - CCNA_00711 0.39 -0.4 769,263 - CCNA_00711 0.39 -0.5 769,263 - CCNA_00711 0.39 -0.6 769,263 - CCNA_00711 0.39 +0.3 769,263 - CCNA_00711 0.39 -1.8 769,305 - CCNA_00711 0.43 -1.4 769,322 + CCNA_00711 0.44 -1.4 769,330 + CCNA_00711 0.45 +1.4 769,345 + CCNA_00711 0.47 -1.4 769,353 - CCNA_00711 0.47 -0.4 769,367 - CCNA_00711 0.49 -0.4 769,415 + CCNA_00711 0.53 -0.6 769,451 + CCNA_00711 0.56 -0.9 769,451 + CCNA_00711 0.56 +0.6 769,456 + CCNA_00711 0.57 -1.0 769,459 - CCNA_00711 0.57 +1.6 769,461 + CCNA_00711 0.57 -0.1 769,461 + CCNA_00711 0.57 +0.7 769,464 - CCNA_00711 0.57 -1.6 769,468 + CCNA_00711 0.58 +1.9 769,468 + CCNA_00711 0.58 -0.0 769,468 + CCNA_00711 0.58 +1.5 769,469 - CCNA_00711 0.58 +0.0 769,469 - CCNA_00711 0.58 +0.0 769,469 - CCNA_00711 0.58 -0.7 769,469 - CCNA_00711 0.58 +0.6 769,498 + CCNA_00711 0.60 -1.5 769,500 + CCNA_00711 0.61 -0.2 769,500 + CCNA_00711 0.61 +0.0 769,508 - CCNA_00711 0.61 -0.4 769,589 - CCNA_00711 0.69 -1.9 769,591 + CCNA_00711 0.69 -0.7 769,591 + CCNA_00711 0.69 +0.3 769,591 + CCNA_00711 0.69 +0.4 769,591 + CCNA_00711 0.69 -1.2 769,591 + CCNA_00711 0.69 +0.0 769,599 - CCNA_00711 0.70 -0.9 769,599 - CCNA_00711 0.70 +0.0 769,599 - CCNA_00711 0.70 -2.4 769,631 - CCNA_00711 0.72 -0.2 769,652 + CCNA_00711 0.74 +0.8 769,660 - CCNA_00711 0.75 -2.0 769,674 + CCNA_00711 0.76 -2.1 769,685 - CCNA_00711 0.77 -1.0
Or see this region's nucleotide sequence