Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27205 and BWI76_RS27210 are separated by 80 nucleotides BWI76_RS27210 and BWI76_RS27215 overlap by 1 nucleotides BWI76_RS27215 and BWI76_RS27220 are separated by 59 nucleotides BWI76_RS27220 and BWI76_RS27225 are separated by 111 nucleotides
BWI76_RS27205: BWI76_RS27205 - serine O-acetyltransferase, at 5,647,277 to 5,648,098
_RS27205
BWI76_RS27210: BWI76_RS27210 - glycerol-3-phosphate dehydrogenase, at 5,648,179 to 5,649,195
_RS27210
BWI76_RS27215: BWI76_RS27215 - protein-export protein SecB, at 5,649,195 to 5,649,662
_RS27215
BWI76_RS27220: BWI76_RS27220 - glutaredoxin 3, at 5,649,722 to 5,649,973
_RS27220
BWI76_RS27225: BWI76_RS27225 - rhodanese-like domain-containing protein, at 5,650,085 to 5,650,516
_RS27225
Position (kb) 5648
5649
5650 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5647.322 kb on + strand at 5648.103 kb on - strand at 5648.103 kb on - strand at 5649.738 kb on - strand at 5649.898 kb on - strand, within BWI76_RS27220 at 5649.958 kb on - strand at 5650.117 kb on - strand at 5650.164 kb on - strand, within BWI76_RS27225 at 5650.175 kb on - strand, within BWI76_RS27225
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 5,647,322 + -3.2 5,648,103 - -0.8 5,648,103 - -0.3 5,649,738 - -0.7 5,649,898 - BWI76_RS27220 0.70 -1.1 5,649,958 - -0.5 5,650,117 - +0.3 5,650,164 - BWI76_RS27225 0.18 -0.0 5,650,175 - BWI76_RS27225 0.21 +1.5
Or see this region's nucleotide sequence