Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27200

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27195 and BWI76_RS27200 are separated by 49 nucleotidesBWI76_RS27200 and BWI76_RS27205 are separated by 105 nucleotides BWI76_RS27195: BWI76_RS27195 - alpha-hydroxy-acid oxidizing enzyme, at 5,645,418 to 5,646,648 _RS27195 BWI76_RS27200: BWI76_RS27200 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 5,646,698 to 5,647,171 _RS27200 BWI76_RS27205: BWI76_RS27205 - serine O-acetyltransferase, at 5,647,277 to 5,648,098 _RS27205 Position (kb) 5646 5647 5648Strain fitness (log2 ratio) -3 -2 -1 0 1at 5645.871 kb on - strand, within BWI76_RS27195at 5645.871 kb on - strand, within BWI76_RS27195at 5645.873 kb on + strand, within BWI76_RS27195at 5645.873 kb on + strand, within BWI76_RS27195at 5646.072 kb on + strand, within BWI76_RS27195at 5646.266 kb on + strand, within BWI76_RS27195at 5646.406 kb on + strand, within BWI76_RS27195at 5646.651 kb on - strandat 5646.652 kb on - strandat 5646.866 kb on + strand, within BWI76_RS27200at 5646.924 kb on - strand, within BWI76_RS27200at 5646.966 kb on + strand, within BWI76_RS27200at 5647.005 kb on + strand, within BWI76_RS27200at 5647.322 kb on + strandat 5648.103 kb on - strandat 5648.103 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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5,645,871 - BWI76_RS27195 0.37 -2.0
5,645,871 - BWI76_RS27195 0.37 -1.3
5,645,873 + BWI76_RS27195 0.37 -0.8
5,645,873 + BWI76_RS27195 0.37 -2.8
5,646,072 + BWI76_RS27195 0.53 -1.5
5,646,266 + BWI76_RS27195 0.69 -1.1
5,646,406 + BWI76_RS27195 0.80 -0.1
5,646,651 - -1.0
5,646,652 - -0.9
5,646,866 + BWI76_RS27200 0.35 -1.3
5,646,924 - BWI76_RS27200 0.48 -1.0
5,646,966 + BWI76_RS27200 0.57 -1.6
5,647,005 + BWI76_RS27200 0.65 -0.1
5,647,322 + -3.2
5,648,103 - -0.8
5,648,103 - -0.3

Or see this region's nucleotide sequence