Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26090

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26085 and BWI76_RS26090 are separated by 54 nucleotidesBWI76_RS26090 and BWI76_RS26095 are separated by 19 nucleotidesBWI76_RS26095 and BWI76_RS26100 are separated by 10 nucleotides BWI76_RS26085: BWI76_RS26085 - 3-dehydroquinate synthase, at 5,390,178 to 5,391,272 _RS26085 BWI76_RS26090: BWI76_RS26090 - shikimate kinase, at 5,391,327 to 5,391,848 _RS26090 BWI76_RS26095: BWI76_RS26095 - shikimate kinase, at 5,391,868 to 5,392,106 _RS26095 BWI76_RS26100: BWI76_RS26100 - DNA transporter HofQ, at 5,392,117 to 5,393,358 _RS26100 Position (kb) 5391 5392Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7 8 9at 5390.737 kb on - strand, within BWI76_RS26085at 5391.051 kb on - strand, within BWI76_RS26085at 5391.138 kb on - strand, within BWI76_RS26085at 5391.913 kb on - strand, within BWI76_RS26095at 5391.929 kb on - strand, within BWI76_RS26095at 5392.235 kb on - strandat 5392.260 kb on + strand, within BWI76_RS26100at 5392.287 kb on - strand, within BWI76_RS26100at 5392.447 kb on + strand, within BWI76_RS26100at 5392.460 kb on + strand, within BWI76_RS26100at 5392.696 kb on + strand, within BWI76_RS26100at 5392.704 kb on - strand, within BWI76_RS26100

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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5,390,737 - BWI76_RS26085 0.51 +3.6
5,391,051 - BWI76_RS26085 0.80 +8.9
5,391,138 - BWI76_RS26085 0.88 +7.7
5,391,913 - BWI76_RS26095 0.19 +8.3
5,391,929 - BWI76_RS26095 0.26 +7.6
5,392,235 - -0.3
5,392,260 + BWI76_RS26100 0.12 +1.0
5,392,287 - BWI76_RS26100 0.14 +0.7
5,392,447 + BWI76_RS26100 0.27 -2.0
5,392,460 + BWI76_RS26100 0.28 -0.9
5,392,696 + BWI76_RS26100 0.47 -2.6
5,392,704 - BWI76_RS26100 0.47 +0.2

Or see this region's nucleotide sequence