Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25080

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25065 and BWI76_RS25070 overlap by 42 nucleotidesBWI76_RS25070 and BWI76_RS25075 are separated by 71 nucleotidesBWI76_RS25075 and BWI76_RS25080 are separated by 147 nucleotidesBWI76_RS25080 and BWI76_RS25085 overlap by 1 nucleotidesBWI76_RS25085 and BWI76_RS25090 are separated by 91 nucleotides BWI76_RS25065: BWI76_RS25065 - polyribonucleotide nucleotidyltransferase, at 5,218,446 to 5,220,584 _RS25065 BWI76_RS25070: BWI76_RS25070 - hypothetical protein, at 5,220,543 to 5,220,754 _RS25070 BWI76_RS25075: BWI76_RS25075 - 30S ribosomal protein S15, at 5,220,826 to 5,221,095 _RS25075 BWI76_RS25080: BWI76_RS25080 - tRNA pseudouridine(55) synthase, at 5,221,243 to 5,222,187 _RS25080 BWI76_RS25085: BWI76_RS25085 - ribosome-binding factor A, at 5,222,187 to 5,222,588 _RS25085 BWI76_RS25090: BWI76_RS25090 - translation initiation factor IF-2, at 5,222,680 to 5,225,370 _RS25090 Position (kb) 5221 5222 5223Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 5220.430 kb on + strandat 5220.472 kb on + strandat 5220.681 kb on - strand, within BWI76_RS25070at 5220.709 kb on - strandat 5221.250 kb on + strandat 5221.407 kb on - strand, within BWI76_RS25080at 5221.453 kb on + strand, within BWI76_RS25080at 5221.520 kb on + strand, within BWI76_RS25080at 5221.746 kb on - strand, within BWI76_RS25080at 5221.852 kb on + strand, within BWI76_RS25080

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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5,220,430 + +0.2
5,220,472 + +1.8
5,220,681 - BWI76_RS25070 0.65 -2.2
5,220,709 - +2.8
5,221,250 + +0.0
5,221,407 - BWI76_RS25080 0.17 +3.4
5,221,453 + BWI76_RS25080 0.22 +1.6
5,221,520 + BWI76_RS25080 0.29 +2.9
5,221,746 - BWI76_RS25080 0.53 +3.3
5,221,852 + BWI76_RS25080 0.64 +4.0

Or see this region's nucleotide sequence