Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22655 and BWI76_RS22660 overlap by 7 nucleotides BWI76_RS22660 and BWI76_RS22665 overlap by 20 nucleotides BWI76_RS22665 and BWI76_RS22670 overlap by 4 nucleotides BWI76_RS22670 and BWI76_RS22675 overlap by 1 nucleotides
BWI76_RS22655: BWI76_RS22655 - protein-L-isoaspartate O-methyltransferase, at 4,722,495 to 4,723,121
_RS22655
BWI76_RS22660: BWI76_RS22660 - 5'/3'-nucleotidase SurE, at 4,723,115 to 4,723,876
_RS22660
BWI76_RS22665: BWI76_RS22665 - tRNA pseudouridine(13) synthase TruD, at 4,723,857 to 4,724,906
_RS22665
BWI76_RS22670: BWI76_RS22670 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 4,724,903 to 4,725,382
_RS22670
BWI76_RS22675: BWI76_RS22675 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 4,725,382 to 4,726,092
_RS22675
Position (kb) 4723
4724
4725 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4722.901 kb on - strand, within BWI76_RS22655 at 4722.933 kb on + strand, within BWI76_RS22655 at 4722.941 kb on - strand at 4723.281 kb on + strand, within BWI76_RS22660 at 4723.289 kb on - strand, within BWI76_RS22660 at 4723.368 kb on + strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.463 kb on + strand, within BWI76_RS22660 at 4723.637 kb on + strand, within BWI76_RS22660 at 4723.657 kb on + strand, within BWI76_RS22660 at 4723.665 kb on - strand, within BWI76_RS22660 at 4723.685 kb on - strand, within BWI76_RS22660 at 4723.831 kb on - strand at 4724.108 kb on - strand, within BWI76_RS22665 at 4724.504 kb on + strand, within BWI76_RS22665 at 4724.504 kb on + strand, within BWI76_RS22665 at 4724.512 kb on - strand, within BWI76_RS22665 at 4724.614 kb on - strand, within BWI76_RS22665 at 4724.841 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 4,722,901 - BWI76_RS22655 0.65 -0.2 4,722,933 + BWI76_RS22655 0.70 +2.9 4,722,941 - +2.8 4,723,281 + BWI76_RS22660 0.22 -1.0 4,723,289 - BWI76_RS22660 0.23 +0.5 4,723,368 + BWI76_RS22660 0.33 +0.3 4,723,376 - BWI76_RS22660 0.34 -0.6 4,723,376 - BWI76_RS22660 0.34 +1.8 4,723,376 - BWI76_RS22660 0.34 -1.0 4,723,463 + BWI76_RS22660 0.46 -0.6 4,723,637 + BWI76_RS22660 0.69 +1.1 4,723,657 + BWI76_RS22660 0.71 -0.5 4,723,665 - BWI76_RS22660 0.72 +0.6 4,723,685 - BWI76_RS22660 0.75 -0.4 4,723,831 - -2.5 4,724,108 - BWI76_RS22665 0.24 -0.1 4,724,504 + BWI76_RS22665 0.62 -0.5 4,724,504 + BWI76_RS22665 0.62 -1.8 4,724,512 - BWI76_RS22665 0.62 -1.2 4,724,614 - BWI76_RS22665 0.72 -1.2 4,724,841 + -0.3
Or see this region's nucleotide sequence