Experiment: Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22040 and BWI76_RS22045 are separated by 183 nucleotides BWI76_RS22045 and BWI76_RS22050 overlap by 4 nucleotides BWI76_RS22050 and BWI76_RS22055 overlap by 28 nucleotides BWI76_RS22055 and BWI76_RS22060 are separated by 10 nucleotides
BWI76_RS22040: BWI76_RS22040 - carboxymuconolactone decarboxylase family protein, at 4,601,727 to 4,602,158
_RS22040
BWI76_RS22045: BWI76_RS22045 - NrdH-redoxin, at 4,602,342 to 4,602,587
_RS22045
BWI76_RS22050: BWI76_RS22050 - class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI, at 4,602,584 to 4,602,994
_RS22050
BWI76_RS22055: BWI76_RS22055 - ribonucleotide-diphosphate reductase, at 4,602,967 to 4,605,111
_RS22055
BWI76_RS22060: BWI76_RS22060 - ribonucleotide-diphosphate reductase subunit beta, at 4,605,122 to 4,606,084
_RS22060
Position (kb)
4602
4603
4604
4605
4606 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4602.856 kb on - strand, within BWI76_RS22050 at 4602.916 kb on + strand, within BWI76_RS22050 at 4602.924 kb on + strand, within BWI76_RS22050 at 4602.924 kb on + strand, within BWI76_RS22050 at 4602.988 kb on - strand at 4603.129 kb on + strand at 4603.374 kb on - strand, within BWI76_RS22055 at 4603.846 kb on + strand, within BWI76_RS22055 at 4603.861 kb on - strand, within BWI76_RS22055 at 4603.871 kb on + strand, within BWI76_RS22055 at 4603.898 kb on - strand, within BWI76_RS22055 at 4604.163 kb on - strand, within BWI76_RS22055 at 4604.187 kb on - strand, within BWI76_RS22055 at 4604.194 kb on + strand, within BWI76_RS22055 at 4604.321 kb on + strand, within BWI76_RS22055 at 4604.363 kb on + strand, within BWI76_RS22055 at 4604.377 kb on - strand, within BWI76_RS22055 at 4604.377 kb on - strand, within BWI76_RS22055 at 4604.393 kb on + strand, within BWI76_RS22055 at 4604.484 kb on + strand, within BWI76_RS22055 at 4604.620 kb on + strand, within BWI76_RS22055 at 4604.620 kb on + strand, within BWI76_RS22055 at 4604.823 kb on + strand, within BWI76_RS22055 at 4604.868 kb on + strand, within BWI76_RS22055 at 4604.904 kb on + strand at 4604.949 kb on + strand at 4604.959 kb on - strand at 4605.039 kb on + strand at 4605.099 kb on - strand at 4605.139 kb on + strand at 4605.162 kb on + strand at 4605.263 kb on + strand, within BWI76_RS22060 at 4605.264 kb on + strand, within BWI76_RS22060 at 4605.309 kb on + strand, within BWI76_RS22060 at 4605.377 kb on - strand, within BWI76_RS22060 at 4605.377 kb on - strand, within BWI76_RS22060 at 4605.377 kb on - strand, within BWI76_RS22060 at 4605.397 kb on + strand, within BWI76_RS22060 at 4605.430 kb on - strand, within BWI76_RS22060 at 4605.539 kb on - strand, within BWI76_RS22060 at 4605.704 kb on + strand, within BWI76_RS22060 at 4605.704 kb on + strand, within BWI76_RS22060 at 4605.751 kb on + strand, within BWI76_RS22060 at 4605.756 kb on + strand, within BWI76_RS22060 at 4605.765 kb on + strand, within BWI76_RS22060 at 4605.806 kb on + strand, within BWI76_RS22060 at 4605.826 kb on + strand, within BWI76_RS22060 at 4605.834 kb on + strand, within BWI76_RS22060 at 4605.834 kb on - strand, within BWI76_RS22060 at 4605.842 kb on - strand, within BWI76_RS22060 at 4605.842 kb on - strand, within BWI76_RS22060 at 4605.842 kb on - strand, within BWI76_RS22060 at 4605.861 kb on + strand, within BWI76_RS22060 at 4605.864 kb on - strand, within BWI76_RS22060 at 4605.869 kb on - strand, within BWI76_RS22060 at 4605.903 kb on - strand, within BWI76_RS22060 at 4605.968 kb on - strand, within BWI76_RS22060 at 4606.033 kb on - strand at 4606.047 kb on - strand at 4606.068 kb on - strand at 4606.078 kb on + strand at 4606.078 kb on + strand at 4606.084 kb on + strand at 4606.100 kb on + strand at 4606.108 kb on - strand at 4606.110 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM remove 4,602,856 - BWI76_RS22050 0.66 -1.0 4,602,916 + BWI76_RS22050 0.81 -0.2 4,602,924 + BWI76_RS22050 0.83 -1.8 4,602,924 + BWI76_RS22050 0.83 -1.0 4,602,988 - -2.2 4,603,129 + -0.3 4,603,374 - BWI76_RS22055 0.19 -1.9 4,603,846 + BWI76_RS22055 0.41 -1.5 4,603,861 - BWI76_RS22055 0.42 -0.1 4,603,871 + BWI76_RS22055 0.42 -3.4 4,603,898 - BWI76_RS22055 0.43 -1.7 4,604,163 - BWI76_RS22055 0.56 -1.7 4,604,187 - BWI76_RS22055 0.57 +0.0 4,604,194 + BWI76_RS22055 0.57 -2.5 4,604,321 + BWI76_RS22055 0.63 -2.0 4,604,363 + BWI76_RS22055 0.65 -2.8 4,604,377 - BWI76_RS22055 0.66 -2.0 4,604,377 - BWI76_RS22055 0.66 -2.2 4,604,393 + BWI76_RS22055 0.66 -1.7 4,604,484 + BWI76_RS22055 0.71 -1.0 4,604,620 + BWI76_RS22055 0.77 -1.5 4,604,620 + BWI76_RS22055 0.77 -2.2 4,604,823 + BWI76_RS22055 0.87 -0.9 4,604,868 + BWI76_RS22055 0.89 -1.5 4,604,904 + -0.1 4,604,949 + -1.7 4,604,959 - -0.9 4,605,039 + +1.8 4,605,099 - -0.3 4,605,139 + +0.2 4,605,162 + -4.2 4,605,263 + BWI76_RS22060 0.15 -0.7 4,605,264 + BWI76_RS22060 0.15 -0.0 4,605,309 + BWI76_RS22060 0.19 -1.5 4,605,377 - BWI76_RS22060 0.26 -0.2 4,605,377 - BWI76_RS22060 0.26 -2.7 4,605,377 - BWI76_RS22060 0.26 +0.0 4,605,397 + BWI76_RS22060 0.29 -1.0 4,605,430 - BWI76_RS22060 0.32 -1.3 4,605,539 - BWI76_RS22060 0.43 -2.7 4,605,704 + BWI76_RS22060 0.60 -1.1 4,605,704 + BWI76_RS22060 0.60 -0.9 4,605,751 + BWI76_RS22060 0.65 -0.1 4,605,756 + BWI76_RS22060 0.66 -0.7 4,605,765 + BWI76_RS22060 0.67 -1.7 4,605,806 + BWI76_RS22060 0.71 -1.4 4,605,826 + BWI76_RS22060 0.73 -0.0 4,605,834 + BWI76_RS22060 0.74 +3.3 4,605,834 - BWI76_RS22060 0.74 -0.1 4,605,842 - BWI76_RS22060 0.75 -2.6 4,605,842 - BWI76_RS22060 0.75 -3.2 4,605,842 - BWI76_RS22060 0.75 -2.8 4,605,861 + BWI76_RS22060 0.77 -0.8 4,605,864 - BWI76_RS22060 0.77 -0.4 4,605,869 - BWI76_RS22060 0.78 -2.2 4,605,903 - BWI76_RS22060 0.81 -0.7 4,605,968 - BWI76_RS22060 0.88 -0.8 4,606,033 - -1.5 4,606,047 - -4.2 4,606,068 - -1.6 4,606,078 + -0.7 4,606,078 + -0.9 4,606,084 + -0.1 4,606,100 + +0.8 4,606,108 - -0.6 4,606,110 + +0.5
Or see this region's nucleotide sequence