Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS21345

Experiment: Cobalt chloride 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS21335 and BWI76_RS21340 are separated by 184 nucleotidesBWI76_RS21340 and BWI76_RS21345 overlap by 4 nucleotidesBWI76_RS21345 and BWI76_RS21350 overlap by 1 nucleotidesBWI76_RS21350 and BWI76_RS21355 are separated by 32 nucleotides BWI76_RS21335: BWI76_RS21335 - elongation factor 4, at 4,465,994 to 4,467,793 _RS21335 BWI76_RS21340: BWI76_RS21340 - SoxR reducing system protein RseC, at 4,467,978 to 4,468,457 _RS21340 BWI76_RS21345: BWI76_RS21345 - sigma-E factor regulatory protein RseB, at 4,468,454 to 4,469,410 _RS21345 BWI76_RS21350: BWI76_RS21350 - anti-RNA polymerase sigma factor SigE, at 4,469,410 to 4,470,060 _RS21350 BWI76_RS21355: BWI76_RS21355 - RNA polymerase sigma factor RpoE, at 4,470,093 to 4,470,668 _RS21355 Position (kb) 4468 4469 4470Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4467.843 kb on - strandat 4467.997 kb on - strandat 4468.218 kb on - strand, within BWI76_RS21340at 4468.223 kb on - strand, within BWI76_RS21340at 4468.265 kb on + strand, within BWI76_RS21340at 4468.534 kb on - strandat 4468.867 kb on + strand, within BWI76_RS21345at 4468.875 kb on - strand, within BWI76_RS21345at 4469.057 kb on - strand, within BWI76_RS21345at 4469.491 kb on - strand, within BWI76_RS21350at 4469.752 kb on - strand, within BWI76_RS21350at 4469.807 kb on + strand, within BWI76_RS21350at 4470.071 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.5 mM
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4,467,843 - +2.5
4,467,997 - +0.7
4,468,218 - BWI76_RS21340 0.50 +1.0
4,468,223 - BWI76_RS21340 0.51 -2.0
4,468,265 + BWI76_RS21340 0.60 -2.8
4,468,534 - -2.3
4,468,867 + BWI76_RS21345 0.43 -1.1
4,468,875 - BWI76_RS21345 0.44 -1.8
4,469,057 - BWI76_RS21345 0.63 -1.9
4,469,491 - BWI76_RS21350 0.12 -1.9
4,469,752 - BWI76_RS21350 0.53 +0.0
4,469,807 + BWI76_RS21350 0.61 -2.9
4,470,071 - -3.6

Or see this region's nucleotide sequence